A5034601447- Genetic and Kidney Cyst Diseases
- Renal and related cancers
- Immune cells in cancer
- Single-cell and spatial transcriptomics
- Alzheimer's disease research and treatments
- Genetic Neurodegenerative Diseases
- Pluripotent Stem Cells Research
- Animal Virus Infections Studies
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Nerve injury and regeneration
- RNA modifications and cancer
- Mitochondrial Function and Pathology
- Genetics and Neurodevelopmental Disorders
- Ubiquitin and proteasome pathways
- 3D Printing in Biomedical Research
- Mesenchymal stem cell research
- Cystic Fibrosis Research Advances
- Biomedical Research and Pathophysiology
- Hedgehog Signaling Pathway Studies
- Hippo pathway signaling and YAP/TAZ
- Prion Diseases and Protein Misfolding
- Liver physiology and pathology
Hunan Cancer Hospital
2018-2024
University of Southern California
2021-2023
Amgen (United States)
2022-2023
University of Alabama at Birmingham
2007-2023
Broad Center
2023
Central Hospital of Wuhan
2014-2022
Huazhong University of Science and Technology
2014-2022
Central South University
2014-2020
Liaoning University of Traditional Chinese Medicine
2020
Columbia University
2019
Significance Statement Despite abundant research focused on understanding the importance of mouse renal resident macrophages in homeostatic and disease settings, these findings have unknown relevance to higher-order species, including humans, because markers identify a similar population cells across species are lacking. This hinders translating data obtained animal model systems human patients. In this study, authors used single-cell RNA sequencing approach, followed by validation using...
Background/Aims: Programmed death ligand1(PD-L1) plays a role in the development and progression of non-small cell lung cancer (NSCLC). This study aimed to identify miRNA(s) that are responsible for regulation expression PD-L1 NSCLC, investigate cycle NSCLC. Methods: We predicted target miRNA PD-L1, which was miR-140, using online tools TargetScan miBase. In NSCLC cells obtained from clinical specimens, addition A549 NCI-H1650 cultures, western blots were used detect level proteins, while...
Significance Statement Disruption of cilia function before postnatal day 12–14 in mice or renal injury adult with dysfunction results accelerated cyst formation. Macrophages have been implicated promoting formation; however, it is unclear whether infiltrating bone marrow-derived kidney resident macrophages are responsible. The authors show that a specific population juvenile-like present during periods Inhibition macrophage accumulation using colony-stimulating factor 1 receptor kinase...
ABSTRACT Porcine reproductive and respiratory syndrome virus (PRRSV) replicates in the cytoplasm of infected cells, but its nucleocapsid (N) protein localizes specifically to nucleus nucleolus. The mechanism nuclear translocation whether N associates with particular nucleolar components are unknown. In present study, we show by confocal microscopy that PRRSV colocalizes small RNA (snoRNA)-associated fibrillarin. Direct specific interaction fibrillarin was demonstrated vivo mammalian...
The dysregulated macrophage phenotype, as the main cause of colitis, not only enhanced oxidative stress to exacerbate inflammatory responses but was closely related with gut microbial dysbiosis. It needed simultaneously address three issues for effective treatment it satisfied. Here, we developed "three-birds-one-stone" probiotics, named Se@EcN-C2/A2, colitis treatment. Escherichia coli Nissle 1917 (EcN), a clinically approved probiotic, used intracellularly synthesize selenium (Se)...
In our previous studies, we found that expression of polyglutamine-expanded huntingtin in HN33 cells induced sensitization N-methyl-d-aspartate (NMDA) receptors (Sun, Y., Savinainen, A., and Liu, Y. F. (2001) J. Biol. Chem. 276, 24713–24718). Following this study, investigated whether tyrosine phosphorylation NMDA might contribute to the altered property receptors. Expression elevation phosphorylated or activated Src increased targeting PSD-95 (post-synaptic density 95) cell surface...
Huntington's disease (HD) is an autosomal dominant disorder caused by expanded CAG repeat on the short arm of chromosome 4 resulting in cognitive decline, motor dysfunction, and death, typically occurring 15 to 20 years after onset symptoms. Neuropathologically, HD characterized a specific loss medium spiny neurons caudate putamen, as well subsequent neuronal cerebral cortex. The transgenic R6/2 mouse model carries N-terminal fragment human gene (145 155 repeats) rapidly develops some...
Abstract Introduction Huntington’s disease (HD) is an autosomal dominant disorder caused by expanded CAG repeat (greater than 38) on the short arm of chromosome 4, resulting in loss and dysfunction neurons neostriatum cortex, leading to cognitive decline, motor dysfunction, death, typically occurring 15 20 years after onset symptoms. Although effective treatment for HD has remained elusive, current studies using transplants bone-marrow-derived mesenchymal stem cells provides considerable...
Atherosclerosis (AS) is associated with high morbidity and mortality rates currently has no effective treatment. This study was aimed at investigating the role of macrophage exosomes in inflammation apoptosis after HUVEC injury. We established injury model using 100 mg/L oxidized low-density lipoprotein (ox-LDL) or 50 ng/mL tumor necrosis factor-α (TNF-α). Cell proliferation assessed cell counting kit-8 (CCK8) assays, expression miR-221, TNF-α, IL-6, IL-10, IL-1β detected quantitative...
We reported previously that normal Huntingtin is associated with epidermal growth factor receptor (EGF) signaling complex (Liu, Y. F., Deth, C. R., and Devys, D. (1997)<i>J. Biol. Chem.</i> 272, 8121–8124). To investigate the potential role of polyglutamine-expanded in regulation receptor-mediated cellular biological function, we stably transfected full-length containing 16, 48, or 89 polyglutamine repeats into PC12 cells where mechanisms, mediated by nerve (NGF) EGF receptors, are well...
Alzheimer's disease (AD) is characterized by a progressive loss of memory and other cognitive disturbances. The neuropathology AD includes the major hallmarks toxic amyloid-β oligomer accumulation neurofibrillary tangles, as well increased oxidative stress, cholinergic dysfunction, synapse loss, changes in endogenous neurotrophic factors, overall degeneration brain. Adult mesenchymal stem cells (MSCs) offer potential for readily available treatment that would be long lasting, have low...
Abstract Synaptic dysfunction and synapse loss are prominent features in Alzheimer’s disease. Members of the Rho-family guanosine triphosphatases, specifically RhoA, synaptic protein Arc implicated these pathogenic processes. They share a common regulatory molecule, E3 ligase Ube3A/E6-AP. Here, we show that Ube3A is reduced an disease mouse model, Tg2576 mouse, which overexpresses human APP695 carrying Swedish mutation, accumulates Aβ brain. Depletion precedes age-dependent behavioral...
Abstract Aggregates of hyperphosphorylated tau protein are a pathological hallmark more than 20 distinct neurodegenerative diseases, including Alzheimer’s disease, progressive supranuclear palsy, and frontotemporal dementia. While the exact mechanism aggregation is unknown, accumulation aggregates correlates with disease progression. Here we report genome-wide CRISPR screen to identify modulators endogenous for first time. Primary screens performed in SH-SY5Y cells, identified positive...
Kidney macrophages are comprised of both monocyte-derived and tissue resident populations; however, the heterogeneity kidney factors that regulate their poorly understood. Herein, we performed single cell RNA sequencing (scRNAseq), fate mapping, parabiosis to define cellular in healthy mice. Our data indicate mouse kidneys contain four major subsets monocytes two (KRM) including a population with enriched Ccr2 expression, suggesting monocyte origin. Surprisingly, mapping using newly...
Caveolin-1 plays an important role in the pathogenesis of idiopathic pulmonary fibrosis. We previously showed that fluorofenidone (FD), a novel pyridine agent, can attenuate bleomycin-induced experimental fibrosis and restore production caveolin-1. In this study, we explore mainly whether caveolin-1 critical anti-pulmonary effects FD vitro. The normal human lung fibroblasts (NHLFs) were cultured with transforming growth factor-β1 (TGF-β1) then treated FD. Subsequently, NHLFs transfected...
Hepatorenal fibrocystic disease (HRFCD) is characterized by cysts in the kidney and liver with associated fibrosis result of defects proteins required for cilia function or assembly. Previous reports indicate that macrophages, mainly M2-like contribute to HRFCD, although origin these cells (yolk sac-derived resident macrophages vs. bone marrow-derived infiltrating macrophages) their contribution observed phenotypes are unknown. We utilize a congenital model dysfunction (IFT88Orpk) study...
Symmetry plays a fundamental role in condensed matter. The unique entanglement between magnetic sublattices and alternating crystal environment altermagnets provides opportunity for designing space symmetry. There have been extensive experimental efforts concentrated on tuning the Neel vector to reconstruct altermagnetic However, it remains challenging modulate symmetry through aspect. Here, design of altermagnetism is successfully realized, by breaking glide mirrors (0001) crystallographic...
Abstract Based on the critical role of actin in maintenance synaptic function, we examined whether expression familial β‐amyloid precursor protein APP‐V642I (IAPP) or mutant presenilin‐1 L286V (mPS1) affects polymerization rat septal neuronal cells. Expression either IAPP mPS1 but not wild‐type amyloid presenilin‐1induced formation stress fibers SN1 cells, a cell line. Treatment with (Aβ) peptide also caused cells and primary cultured hippocampal neurons. γ‐secretase inhibitor completely...
To investigate the correlation of miR-125a/b expression with acute exacerbations chronic obstructive pulmonary disease (AECOPD) patients and inflammatory cytokines. Eighty-seven AECOPD patients, 93 stable (COPD) 100 health volunteers (HCs) were recruited. Plasma samples collected from at day 1, 7, 14, 28 admission COPD as well HCs. Total RNA was extracted plasma, relative expressions determined by quantitative real time-polymerase chain reaction. MiR-125b had a great capacity for...
ABSTRACT The transition zone (TZ) is a domain at the base of cilium that involved in maintaining ciliary compartment‐specific sensory and signaling activity by regulating cilia protein composition. Mutations TZ proteins result dysfunction, often causing pleiotropic effects observed group human diseases classified as ciliopathies. purpose this study to describe importance component Meckel‐Grüber syndrome 6 ( Mks6 ) several organ systems tissues regarding ciliogenesis maintenance using...
Induced pluripotent stem cells (iPSCs) offer certain advantages over embryonic in cell replacement therapy for a variety of neurological disorders. However, reliable procedures, whereby transplanted iPSCs can survive and differentiate into functional neurons, without forming tumors, have yet to be devised. Currently, retroviral or lentiviral reprogramming methods are often used reprogram somatic cells. Although the use these viruses has proven effective, formation tumors results following...