A5040420737- Antibiotics Pharmacokinetics and Efficacy
- Natural product bioactivities and synthesis
- Phytochemistry Medicinal Plant Applications
- Heavy Metals in Plants
- Agricultural and Food Sciences
- Seed and Plant Biochemistry
- Chronic Myeloid Leukemia Treatments
- Advances in Cucurbitaceae Research
- Healthcare during COVID-19 Pandemic
- Plant-based Medicinal Research
- Antioxidants, Aging, Portulaca oleracea
- Alkaloids: synthesis and pharmacology
- Berberine and alkaloids research
- Phytochemicals and Antioxidant Activities
- Prostate Cancer Treatment and Research
- Plant biochemistry and biosynthesis
- Pharmacological Effects of Natural Compounds
- Treatment of Major Depression
- Sensory Analysis and Statistical Methods
- Psidium guajava Extracts and Applications
- Organometallic Compounds Synthesis and Characterization
- Nail Diseases and Treatments
- Fungal Plant Pathogen Control
- Mosquito-borne diseases and control
- Ginger and Zingiberaceae research
Universidade Feevale
2017-2024
Universidade Federal do Rio Grande do Sul
2007-2013
The adjuvant activity of Chenopodium quinoa (quinoa) saponins on the humoral and cellular immune responses mice subcutaneously immunized with ovalbumin (OVA) was evaluated. Two saponin fractions were obtained, FQ70 FQ90, 10 determined by UPLC/Q-TOF-MS. Mice OVA alone or adjuvanted Quil A (adjuvant control), FQ70, FQ90. FQ90 significantly enhanced amount anti-OVA-specific antibodies in serum (IgG, IgG1, IgG2b) mice. effect greater than that However, delayed type hypersensitivity higher...
Ilex paraguariensis A. St. Hilaire (mate) has traditionally been used in several South American countries to prepare tea-like beverages having stimulant effects on the CNS and appetite. In recent years, however, mate preparations have recommended putatively as an appetite suppressant slimming remedy. Moreover, studies carried out either normal or diet-induced obese rats treated with extracts revealed anti-obesity satiety effects, thus refuting ethnopharmacological data. this work, effect of...
The triterpene chikusetsusaponin IVa was isolated from the fruit of Ilex paraguariensis. Using biochemical and pharmacological methods, we demonstrated that (1) prolongs recalcification time, prothrombin activated partial thromboplastin thrombin time normal human plasma in a dose-dependent manner, (2) inhibits amidolytic activity factor Xa upon synthetic substrates S2238 S2222, (3) thrombin-induced fibrinogen clotting (50% inhibition concentration, 199.4 ± 9.1 μM), (4) thrombin-...
A MODEL.The main analytical variables of a modified Folin-Ciocalteu method were studied by UV-Vis and gradient HPLC-PDA methods, using purified (PC) technical grade (TGC) casein.Rutin an aqueous extract Psidium guajava L. leaves used as models.The best results ascribed to TGC.Certainly PC bonds the polyphenols P. better than TGC, but TGC afforded precision.A lack specificity occurred when rutin was analyzed together with P extract.Additional analyses performed flavonoid fraction had...
The antitumor activity of <i>Uncaria tomentosa</i>, a native vine from the Amazonian rainforest, has been ascribed to pentacyclic oxindole alkaloids occurring in its bark. Former studies have shown that this activity, as well intensity, depends on whether cat's claw occur original compounds or isomerized derivatives. This work addresses aspect, using T24 and RT4 human bladder cancer cell lines for purpose. Bark samples were extracted by dynamic maceration, prepurified with cross-linked...
ABSTRACT Introduction Ilex paraguariensis A. St. Hil. (mate) is known in several South American countries because of the use its leaves stimulant herbal beverages. High saponin contents were reported mate and unripe fruits that possess a dissimilar composition. Two LC‐UV methods previously for saponins assay focused on quantification less polar fraction fruits. Objective To develop validate method to total content characterise chemical structure triterpenic by UPLC/Q‐TOF‐MS. Methodology From...
Cat's claw oxindole alkaloids are prone to isomerization in aqueous solution. However, studies on their behavior extraction processes scarce. This paper addressed the issue by considering five commonly used processes. Unlike dynamic maceration (DM) and ultrasound-assisted extraction, substantial was induced static maceration, turbo-extraction reflux extraction. After heating under DM, kinetic order of established equations were fitted successfully using a four-parameter Weibull model (R² >...
Cross-povidone, or polyvinylpyrrolidone (PVPP) affords an analytical alternative instead of hide-powder and casein as binding agent in the content assay vegetable tannins. In this work we studied specificity PVPP to bind polyphenolics presence flavonoids. Gallic acid, tannic catechin pyrogallol were used polyphenolic reference substances (PRS), along with aqueous extract from Psidium guajava L. leaves. The was assayed by UV-Vis High Performance Liquid Chromatography-Photodiode array...
Abstract Imatinib mesylate (IM) is an anti‐neoplasic drug used for the treatment of cancer. Recent new guidelines specify daily doses and concentration limits genotoxic impurities (GTIs) in pharmaceutical final products. Therefore, this work analytical method using UHPLC–MS/MS was developed, validated applied to characterize IM tablets two GTIs: N‐ (2‐methyl‐5‐aminophenyl)‐4‐(3‐pyridyl)‐2‐pyrimidine amine (Imp. 1), [4‐methyl‐3‐(4‐methyl‐3‐yl‐pyrimidin‐2‐ylamino)‐phenyl]‐4‐ chloromethyl...
Yellow Fever Virus (YFV) and Chikungunya (CHIV) are neglected reemerging pathogens that cause comorbidities worldwide. Since no antiviral drug is prescribed for those infections, there a demand on researching compounds inhibit viral replication. Saponins amphiphilic already demonstrated in vitro activity against enveloped virus. Therefore, two purified saponin fractions from Quillaja spp. were evaluated regarding their potential YFV CHIKV. The cell line used this study was VERO (African...
Abstract Abiraterone acetate efficacy against prostate cancer is dependent on the circulating levels of abiraterone and its active metabolites, which present significant pharmacokinetic variability among patients. Thus, therapeutic drug monitoring can be performed to improve treatment outcomes. To support such studies, there are only a limited number bioanalytical methods in current literature. This work presents fast method quantify D4A plasma 4 min by UPLC–MS/MS. Bioanalytical validation...
Imatinib mesylate (IM) is a widely used anticancer drug, mainly for treating chronic myeloid leukemia. However, pharmaceutical formulations may contain impurities, which in some cases can be more toxic than the parent compounds. This study aimed to compare toxicity of IM and one its impurities (IMP), N-(2-methyl-5-aminophenyl)-4-(3-pyridyl)-2-pyrimidine amine, using progressively complex models. Cytotoxicity was evaluated 3-(4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium, neutral red...
Abstract A fast and sensitive high performance chromatographic method for quantification of sodium alendronate was developed afterward implemented tablet content dissolution test. Sodium reference standard samples two different commercial tablets were used. The analysis performed using an anion exchange column at 30°C a mixture nitric acid 1.5 mM copper II nitrate 0.5 (50:50, v/v) as the mobile phase. run time 15 minutes detection measured 240 nm. limit approximately 0.3 µg mL−1 0.9 mL−1....
*** Oxidative stress in patients with type 2 diabetes mellitus treated metformin ***AIMS: To evaluate oxidative parameters metformin, relating these values to its side effects, plasma levels, glycemic control, diabetic complications, lipid profile, and the influence of pharmacotherapeutic follow-up.METHODS: Patients mellitus, on follow-up for four months, were evaluated. The consisted providing information answering patients’ questions about medication disease. In addition, administration...