A5081865083- Nanoparticle-Based Drug Delivery
- Lipid Membrane Structure and Behavior
- Alzheimer's disease research and treatments
- Advanced Drug Delivery Systems
- Gold and Silver Nanoparticles Synthesis and Applications
- Nanoplatforms for cancer theranostics
- RNA Interference and Gene Delivery
- Drug Transport and Resistance Mechanisms
- Nanocluster Synthesis and Applications
- Supramolecular Self-Assembly in Materials
- Air Quality and Health Impacts
- Barrier Structure and Function Studies
- Cholesterol and Lipid Metabolism
- Inhalation and Respiratory Drug Delivery
- Molecular Communication and Nanonetworks
- Advancements in Transdermal Drug Delivery
- Advanced Polymer Synthesis and Characterization
- Cancer Research and Treatment
- Climate Change and Health Impacts
- Ion Transport and Channel Regulation
- Cell death mechanisms and regulation
- Microwave-Assisted Synthesis and Applications
- Advanced Electron Microscopy Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- Computational Drug Discovery Methods
Instituto de Ciencia de Materiales de Madrid
2024
University of Milano-Bicocca
2016-2021
Engineered nanoparticles offer the chance to improve drug transport and delivery through biological barriers, exploiting possibility leave blood circulation traverse endothelial vascular bed, blood–brain barrier (BBB) included, reach their target. It is known that gather molecules on surface upon contact with fluids, forming "protein corona", which can affect fate therapeutic/diagnostic performance, yet no information corona's evolution across has been gathered so far. Using a cellular model...
Much evidence suggests a protective role of high-density lipoprotein (HDL) and its major apolipoprotein apoA-I, in Alzheimer's disease (AD). The biogenesis nascent HDL derived from first lipidation which is synthesized by the liver intestine but not brain, process mediated ABCA1. maturation mature spherical due to subsequent step, LCAT-mediated cholesterol esterification, change apoA-I conformation. Therefore, different subclasses apoA-I-HDL simultaneously exist blood circulation. Here, we...
Nanoparticles may provide a viable way for neuroprotective drugs to cross the blood–brain barrier (BBB), which limits passage of most from peripheral circulation brain. Heterotelechelic polymer prodrugs comprising model drug (adenosine) and maleimide functionality were synthesized by "drug-initiated" approach subsequent nitroxide exchange reaction. obtained nanoprecipitation exhibited high colloidal stability with diameters in 162–185 nm range narrow size distributions. then covalently...
Despite advances in cancer therapies, nanomedicine approaches including the treatment of glioblastoma (GBM), most common, aggressive brain tumor, remains inefficient. These failures are likely attributable to complex and not yet completely known biology this which is responsible for its strong invasiveness, high degree metastasis, proliferation potential, resistance radiation chemotherapy. The intimate connection through cells communicate between them plays an important role these biological...
H-ferritin nanoconjugates of CTX and TZ are developed as carriers across the BBB to allow immunotherapy primary glioblastoma HER2+ breast cancer brain metastases. The reliability strategy is demonstrated using a transwell barrier model.
Abstract We designed liposomes dually functionalized with ApoE-derived peptide (mApoE) and chlorotoxin (ClTx) to improve their blood–brain barrier (BBB) crossing. Our results demonstrated the synergistic activity of ClTx-mApoE in boosting doxorubicin-loaded across BBB, keeping anti-tumour drug loaded: mApoE acts promoting cellular uptake, while ClTx promotes exocytosis liposomes.
Purpose: Nanotechnologies turned out to be promising in the development of diagnostic and therapeutic approaches toward neurodegenerative disorders. However, only a very scant number nanodevices until now proved effective on preclinical animal models. Although specific tests vivo are available assess potential toxicity these cognitive functions, those evaluate their biosafety vitro neurons still improved. Materials methods: We utilized patch-clamp technique primary cultures cortical neural...
The cerebral synthesis of cholesterol is mainly handled by astrocytes, which are also responsible for apoproteins’ and lipoproteins’ assembly required the transport in brain parenchyma. In Alzheimer disease (AD), these processes impaired, likely because astrogliosis, a process characterized morphological functional changes astrocytes. Several ATP-binding cassette transporters expressed cells involved formation nascent discoidal lipoproteins, but effect beta-amyloid (Aβ) assemblies on this...
Abstract In the pursuit of enhancing cancer treatment efficacy while minimizing side effects, near‐infrared (NIR) photothermal therapy (PTT) has emerged as a promising approach. By using photothermally active nanomaterials, PTT enables localized hyperthermia, effectively eliminating cells with minimal invasiveness and toxicity. Among these gold nanostars (AuNS) stand out due to their tunable plasmon resonance efficient light absorption. This study addresses challenge measuring nanoscale...
Aim: To investigate if and how the ability of liposomes, previously designed for Alzheimer's therapy, to reach brain changes in aging/pathological conditions with respect healthy state. Methods: Biodistribution pharmacokinetics liposomes young or aged mice an mouse model were measured by radiochemical techniques. The expression receptors structural proteins was evaluated Western blot. Results: At equal blood levels, amount integrity dramatically lower mice, animals. These differences are...
The deposition of amyloid-β (Aβ) plaques in the brain is a significant pathological signature Alzheimer’s disease, correlating with synaptic dysfunction and neurodegeneration. Several compounds, peptides, or drugs have been designed to redirect stop Aβ aggregation. Among them, trideca-peptide CWG-LRKLRKRLLR (mApoE), which derived from receptor binding sequence apolipoprotein E, effectively able inhibit aggregation promote fibril disaggregation. Taking advantage Atomic Force Microscopy (AFM)...
Vascular oxidative stress is considered a worsening factor in the progression of Alzheimer’s disease (AD). Increased reactive oxygen species (ROS) levels promote accumulation amyloid-β peptide (Aβ), one main hallmarks AD. In turn, Aβ potent inducer stress. early stages AD, concomitant action and on brain capillary endothelial cells was observed to compromise blood–brain barrier functionality. this context, antioxidant compounds might provide therapeutic benefits. To aim, we investigated...
The cell microenvironment plays a pivotal role in mediating adhesion, survival, and proliferation physiological pathological states. relevance of extracellular matrix (ECM) proteins fate control is an important issue to take into consideration for both tissue engineering biology studies. glycosylation ECM remains, however, largely unexplored. In order investigate the physio-pathological effects differential glycosylation, design affordable chemoselective methods components desirable. We will...