A5032501205- Gut microbiota and health
- T-cell and B-cell Immunology
- Clostridium difficile and Clostridium perfringens research
- Immune Cell Function and Interaction
- Tryptophan and brain disorders
- Multiple Sclerosis Research Studies
- Immune Response and Inflammation
- Immunotherapy and Immune Responses
- Probiotics and Fermented Foods
- Diet and metabolism studies
- Gastrointestinal motility and disorders
- Viral gastroenteritis research and epidemiology
- IL-33, ST2, and ILC Pathways
- Dermatology and Skin Diseases
- Bacteriophages and microbial interactions
- Salmonella and Campylobacter epidemiology
- Neuroinflammation and Neurodegeneration Mechanisms
- Mycobacterium research and diagnosis
- Ginseng Biological Effects and Applications
- Plant biochemistry and biosynthesis
- Reproductive System and Pregnancy
- Inflammatory Bowel Disease
- Natural product bioactivities and synthesis
- Metabolism and Genetic Disorders
- GABA and Rice Research
Boise State University
2022-2025
Eastern Washington University
2016-2022
Washington State University Spokane
2018-2021
Universidad de Navarra
2004-2021
Dartmouth College
2008-2017
Hanover College
2014-2015
Dartmouth Hospital
2014-2015
Sanford Burnham Prebys Medical Discovery Institute
2014
University of California, Santa Barbara
2014
Montana State University
2005-2012
Abstract Mucosal tolerance has been considered a potentially important pathway for the treatment of autoimmune disease, including human multiple sclerosis and experimental conditions such as encephalomyelitis (EAE). There is limited information on capacity commensal gut bacteria to induce maintain peripheral immune tolerance. Inbred SJL C57BL/6 mice were treated orally with broad spectrum antibiotics reduce microflora. Reduction impaired development EAE. Intraperitoneal antibiotic-treated...
Abstract The importance of gut commensal bacteria in maintaining immune homeostasis is increasingly understood. We recently described that alteration the microflora can affect a population Foxp3+Treg cells regulate demyelination experimental autoimmune encephalomyelitis (EAE), model human multiple sclerosis. now extend our previous observations on role CNS demyelination, and we demonstrate Bacteroides fragilis producing bacterial capsular polysaccharide Ag protect against EAE. Recolonization...
Polysaccharide A (PSA) derived from the human commensal Bacteroides fragilis is a symbiosis factor that stimulates immunologic development within mammalian hosts. PSA rebalances skewed systemic T helper responses and promotes regulatory cells (Tregs). However, PSA-mediated induction of Foxp3 in humans has not been reported. In mice, PSA-generated Foxp3+ Tregs dampen Th17 activity thereby facilitating bacterial intestinal colonization while increased presence function these may guard against...
We have recently shown that alteration of the gut commensal microbiota with antibiotics can modify susceptibility to autoimmune demyelinating processes central nervous system. Treatment mice a broad spectrum not only induced significant changes in regulatory T cell populations associated lymphoid tissues (GALT) and peripheral organs but reduced EAE, most widely used animal model for human multiple sclerosis. Here, we show further oral antibiotic treatment EAE CD5(+)B subpopulation conferred...
Tolerance established by host-commensal interactions regulates host immunity at both local mucosal and systemic levels. The intestinal commensal strain Bacteroides fragilis elicits immune tolerance, least in part, via the expression capsular polysaccharide A (PSA). How such niche-specific microbial elements regulate extra-intestinal responses, as brain, remains largely unknown. We have recently shown that oral treatment with PSA suppresses neuro-inflammation elicited during experimental...
The mechanisms that underlie the potent Th1-adjuvant capacity of poly(methyl vinyl ether-co-maleic anhydride) nanoparticles (NPs) were investigated. Traditionally, polymer NPs have been considered delivery systems promote a closer interaction between antigen and antigen-presenting cells (APCs). Our results revealed poly(anhydride) also act as agonists various Toll-like receptors (TLRs) (TLR2, -4, -5), triggering Th1-profile cytokine release (gamma interferon [IFN-gamma], 478 pg/ml versus...
The gut microbiome plays an important role in the development of inflammatory disease as shown using experimental models central nervous system (CNS) demyelination. Gut microbes influence response regulatory immune cell populations gut-associated lymphoid tissue (GALT), which drive protection acute and chronic autoimmune encephalomyelitis (EAE). Recent observations suggest that communication between host is bidirectional. We hypothesized microbiota differs progressive stages a murine model...
Abstract Treatment with an anti-inflammatory Salmonella vaccine expressing enterotoxigenic Escherichia coli colonization factor Ag 1 (CFA/I) proved effective in stimulating protective, potent CD25+CD4+ regulatory T (Treg) cells susceptible mice challenged experimental autoimmune encephalomyelitis (EAE). Because the vector was considerably less we questioned whether altering fimbrial subunit expression to resemble conventional may impact Treg cell potency. The Salmonella-CFA/I modified limit...
Abstract Regulatory T (Treg) cells show promise for treating autoimmune diseases, but their induction to elevated potency has been problematic when the most optimally derived are from diseased animals. To circumvent reliance on autoantigen-reactive Treg cells, stimulation myelin-independent Ags may offer a viable alternative while maintaining treat experimental encephalomyelitis (EAE). The Salmonella vaccine expressing colonization factor Ag I possesses anti-inflammatory properties and,...
Abstract Mucosal tolerance induction generally requires multiple or large Ag doses. Because microfold (M) cells have been implicated as being important for mucosal and because reovirus attachment protein σ1 (pσ1) is capable of binding M cells, we postulated that targeting a model to via pσ1 could induce state unresponsiveness. Accordingly, genetic fusion between OVA the cell ligand, pσ1, termed OVA-pσ1, was developed enhance tolerogen uptake. When applied nasally, not parenterally, little...