A5074760780- Antibiotic Resistance in Bacteria
- Bacterial Identification and Susceptibility Testing
- Antibiotic Use and Resistance
- Genomics and Phylogenetic Studies
- Enterobacteriaceae and Cronobacter Research
- Genetic diversity and population structure
- Mycobacterium research and diagnosis
- Microbial Metabolic Engineering and Bioproduction
- RNA and protein synthesis mechanisms
- Infections and bacterial resistance
- Evolution and Genetic Dynamics
- Bacterial Genetics and Biotechnology
- Yersinia bacterium, plague, ectoparasites research
- Antimicrobial Resistance in Staphylococcus
- Genetic Associations and Epidemiology
The Alfred Hospital
2023-2024
Monash University
2020-2024
Australian Regenerative Medicine Institute
2022
Abstract Background Resistance to third-generation cephalosporins, often mediated by extended-spectrum beta-lactamases (ESBLs), is a considerable issue in hospital-associated infections as few drugs remain for treatment. ESBL genes are located on large plasmids that transfer horizontally between strains and species of Enterobacteriaceae frequently confer resistance additional drug classes. Whilst plasmid transmission recognised occur the hospital setting, frequency impact infection burden,...
Abstract Infections caused by metallo-beta-lactamase-producing organisms (MBLs) are a global health threat. Our understanding of transmission dynamics and how MBLs establish endemicity remains limited. We analysed two decades bla IMP-4 evolution in hospital using sequence data from 270 clinical environmental isolates (including 169 completed genomes) identified the gene across 7 Gram-negative genera, 68 bacterial strains distinct plasmid types. showed an initial multi-species outbreak...
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Abstract RNAseq data can be used to infer genetic variants, yet its use for estimating population structure remains underexplored. Here, we construct a freely available computational tool (RGStraP) estimate RNAseq-based principal components (RG-PCs) and assess whether RG-PCs control in gene expression analyses. Using whole blood samples from understudied Nepalese populations the Geuvadis study, show that had comparable results paired array-based genotypes, with high genotype concordance...
Abstract Objectives: New Delhi metallo-β-lactamases (NDMs) are major contributors to the spread of carbapenem resistance globally. In Australia, NDMs were previously associated with international travel, but from 2019 we noted increasing incidence NDM-positive clinical isolates. We investigated and genomic epidemiology NDM carriage at a tertiary-care Australian hospital 2016 2021. Methods: identified 49 patients 84 NDM-carrying isolates in an institutional database, collected data electronic...
Abstract Background Resistance to third-generation cephalosporins, often mediated by extended-spectrum beta-lactamases (ESBLs), is a considerable issue in hospital-associated infections as few drugs remain for treatment. ESBL genes are located on large plasmids that transfer horizontally between strains and species of Enterobacteriaceae frequently confer resistance additional drug classes. While plasmid transmission recognised occur the hospital setting, frequency impact infection burden,...
ABSTRACT In this study, the genetic differences and clinical impact of carbapenemase-encoding genes among community healthcare-acquired infections were assessed. This retrospective, multicenter cohort study was conducted in Colombia included patients infected with carbapenem-resistant Gram-negative rods between 2017 2021. Carbapenem resistance identified by Vitek, whole-genome sequencing (WGS) to classify alleles sequence types (STs). Descriptive statistics used determine association any...
Abstract Infections caused by metallo-beta-lactamase-producing organisms (MBLs) are a global health threat. Our understanding of transmission dynamics and how MBLs establish endemicity remains limited. We analysed two decades bla IMP-4 evolution in hospital using sequence data from 270 clinical environmental isolates (including 169 completed genomes) identified extreme gene promiscuity across 7 Gram-negative genera, 68 bacterial strains distinct plasmid types. An initial multi-species...
Abstract Motivation Klebsiella species are increasingly multidrug resistant pathogens affecting human and animal health widely distributed in the environment. Among these, pneumoniae complex (KpSC), which includes seven phylogroups, is an important cause of community hospital infections. In addition, oxytoca (KoSC) also causes infections antibiotic-associated haemorrhagic colitis. The unsuitability used clinical microbiology methods to distinguish within each these complexes leads high rates...
Abstract RNAseq data can be used to infer genetic variants, yet its use for estimating population structure remains underexplored. Here, we construct a freely available computational tool (RGStraP) estimate RNAseq-based principal components (RG-PCs) and assess whether RG-PCs control in gene expression analyses. Using whole blood samples from understudied Nepalese populations the Geuvadis study, show that had comparable results paired array-based genotypes, with high genotype concordance...
Abstract The Klebsiella pneumoniae species complex (KpSC) is a set of seven taxa which are found in variety niches, and an important cause opportunistic healthcare-associated infections humans. Due to increasing rates multi-drug resistance within the KpSC, there growing interest better understanding biology metabolism these organisms inform novel control strategies. We collated 37 sequenced KpSC isolates isolated from representing all taxa. generated strain-specific genome scale metabolic...
Abstract Objectives New Delhi metallo-beta-lactamases (NDMs) are major contributors to the spread of carbapenem resistance globally. In Australia, NDMs were previously associated with international travel but from 2019 we noted increasing NDM episodes. We conducted an investigation determine clinical and genomic epidemiology NDM-carriage at a tertiary Australian hospital 2016-2021. Methods identified 49 patients 84 NDM-carrying isolates in institutional database collected data electronic...