Abstract Cotton is an economically important crop throughout the world and a pioneer in salt stress tolerance research. Investigation of genetic regulation salinity will provide information for stress-resistant breeding. Here, we employed next-generation RNA-Seq technology to elucidate salt-tolerant mechanisms cotton using diploid species Gossypium davidsonii which has superior tolerance. A total 4744 5337 differentially expressed genes (DEGs) were found be involved roots leaves,...

Plasmodesmata (PDs) play vital roles in cell-to-cell communication and plant development. Emerging evidence suggests that sterols are involved PD activity during cytokinesis. However, whether contribute to gating between established cells remains unknown. Here, we isolated GhSCP2D, a putative sterol carrier protein gene from elongating cotton (Gossypium hirsutum) fibers. In contrast wild-type fiber PDs, which opened at 5 10 d postanthesis (DPA) closed only 15 25 DPA, plants with suppressed...

Global climate changes cause an increase of abiotic and biotic stresses that tremendously threaten the world's crop security. However, studies on broad-spectrum response pathways involved in are relatively rare. Here, by comparing time-dependent transcriptional co-expression analysis cotton (Gossypium hirsutum) root tissues under stress conditions, we discovered common stress-responsive genes metabolism different stresses, which included circadian rhythm, thiamine galactose metabolism,...

Abstract β-1,3-glucanase functions in plant physiological and developmental processes. However, how participates cell wall development remains largely unknown. Here, we answered this question by examining the role of GhGLU18, a β-1,3-glucanase, cotton (Gossypium hirsutum) fibers, which content β-1,3-glucan changes dynamically from 10% mass at onset secondary deposition to <1% maturation. GhGLU18 was specifically expressed fiber with higher expression late elongation (SCW) synthesis...

COBRA-Like (COBL) genes, which encode a plant-specific glycosylphosphatidylinositol (GPI) anchored protein, have been proven to be key regulators in the orientation of cell expansion and cellulose crystallinity status. Genome-wide analysis has performed A. thaliana, O. sativa, Z. mays S. lycopersicum, but little Gossypium. Here we identified 19, 18 33 candidate COBL genes from three sequenced cotton species, diploid G. raimondii, arboreum tetraploid hirsutum acc. TM-1, respectively. These...

Inhibition of phosphodiesterase 2 and 4 (PDE2A PDE4) increases the intracellular cAMP and/or cGMP levels, which may prevent Amyloid β 42 oligomers (Aβ) related cognitive impairment dementias. Baicalein, one natural flavones found in root Scutellaria baicalensis Georgi, has a wide range pharmacological activities including antioxidant anti-inflammatory effects. However, no studies suggest whether baicalein mediated anti-Alzheimer's disease (AD) events involve PDEs subtypes-mediated...

Programmed cell death (PCD) is an important form to protect plants from pathogen attack. However, must precisely control the PCD process under microbe attacks avoid detrimental effects. The complexity of how balance defense activation and requires further clarification. Lesion mimic mutants constitute excellent material study crosstalk between them. Here, we identified a Gossypium hirsutum (cotton) lesion mutant (Ghlmm), which exhibits necrotic leaf damage enhanced disease resistance....

Abstract Cell elongation is a fundamental process for plant growth and development. Studies have shown lipid metabolism plays important role in cell elongation; however, the related functional mechanisms remain largely unknown. Here, we report that cotton (Gossypium hirsutum) LIPID TRANSFER PROTEIN4 (GhLTP4) promotes fiber via elevating ceramides (Cers) content activating auxin-responsive pathways. GhLTP4 was preferentially expressed elongating fibers. Over-expression down-regulation of led...

SUMMARY Drought has a severe impact on the quality and yield of cotton. Deciphering key genes related to drought tolerance is important for understanding regulation mechanism stress breeding drought‐tolerant cotton cultivars. Several studies have demonstrated that NAC transcription factors are crucial in stress, however, functional mechanisms still largely unexplored. Here, we identified factor GhNAC4 positively regulated The expression was significantly induced by abiotic plant hormones....

Mitochondrial calcium uniporter (MCU) is a conserved ion (Ca2+) transporter in the mitochondrial inner membrane of eukaryotic cells. How MCU proteins regulate Ca2+ flow and modulate plant cell development remain largely unclear. Here, we identified gene GhMCU4 encoding protein that negatively regulates fiber elongation cotton (Gossypium hirsutum). expressed constitutively various tissues with higher transcripts elongating Knockdown significantly elevated height root length. The signaling...

Towards the aim of examining potential function KORRIGAN (KOR), a highly conserved membrane-bound endoglucanase, in reproductive development, here transgenic evidence is provided that cotton (Gossypium hirsutum) GhKOR1, plays significant roles endosperm and embryo development. RNA interference (RNAi)- co-suppression-mediated down-regulation GhKOR1 resulted smaller filial tissue reduced seed weight, which were characterized by disrupted cellularization delayed leading to germination weak...

Abstract Verticillium dahliae infection leads to wilt in cotton and other dicotyledon crops. To reduce the loss of economic crops, more attention has been focused on key genes involved pathogenicity this soil‐borne plant fungal pathogen. Sho1 encodes a conserved tetraspan transmembrane protein which is element two upstream branches HOG ‐ MAPK pathway fungi. required for full virulence wide variety pathogenic In study, sho1 mutant V. (designated ΔVdsho1 ) was generated by Agrobacterium...

The NAC (NAM, ATAF1/2, and CUC2) transcription factor family participates in responses to various kinds of environmental stimuli plants. However, the roles protein cold resistance, especially resistance tomatoes, are not completely understood. This study examined a tomato (Solanum lycopersicum) (SlNAC35) resisting chilling using transgenic tomatoes. GUS staining expression analysis revealed that SlNAC35 was induced at 4 °C, thereby suggesting its involvement plant stress. Moreover, lines...

Abstract Pentatricopeptide repeat (PPR) proteins encoded by nuclear genomes can bind to organellar RNA and are involved in the regulation of metabolism. However, functions many PPR remain unknown plants, especially polyploidy crops. Here, through a map-based cloning strategy Clustered regularly interspaced short palindromic repeats/cas9 (CRISPR/cas9) gene editing technology, we cloned verified an allotetraploid cotton immature fiber (im) mutant (GhImA) encoding protein chromosome A03, that...

Abstract Gossypium barbadense, which is one of several species cotton, well known for its superior fiber quality. However, the genetic basis high-quality remains largely unexplored. Here, we resequenced 269 G. barbadense accessions. Phylogenetic structure analysis showed that set accessions was clustered into 3 groups: G1 and G2 mainly included modern cultivars from Xinjiang, China, G3 related to widely introduced in different regions worldwide. A genome-wide association study 5 quality...

Plant cell walls are complex and dynamic cellular structures critical for plant growth, development, physiology, adaptation. Cellulose is one of the most important components wall. However, how cellulose microfibrils deposit assemble into crystalline remains elusive. The COBRA-LIKE (COBL) plant-specific protein family plays vital roles in modulating deposition orientation microfibril walls. Here, we investigate role GhCOBL9 cotton (Gossypium hirsutum) fiber an ideal model studying elongation...