Abstract Despite the importance of circulating microparticles in haemostasis and thrombosis, there is limited evidence for potential causative effects naturally produced cell-derived on fibrin clot formation its properties. We studied significance blood formation, structure susceptibility to fibrinolysis by removing them from platelet-free plasma using filtration. Clots made microparticle-depleted samples same healthy donors were analyzed parallel. Microparticles accelerate polymerisation...

The microtubule-targeting agents (MTAs) are well-known chemotherapeutic commonly used for therapy of a broad spectrum human malignancies, exhibiting epithelial origin, including breast, lung, and prostate cancer. Despite the impressive response rates shortly after initiation MTA-based therapy, vast majority malignancies develop resistance to MTAs due different mechanisms. Here, we report that infigratinib (BGJ 398), potent FGFR1-4 inhibitor, restores sensitivity ABCB1-overexpressing cancer...

Allergic rhinitis (AR) is especially prevalent among the population of large cities. Immunologically, airway epithelium a region where allergen-presenting cells concentrates. These actively express group receptors innate immune system. A specific cytokine profile its representation. The study was aimed at evaluating in patients with seasonal and perennial allergic rhinitis. nasal secretion blood serum 44 AR studied. 24 them had (SAR), 20 suffered from (PAR). patients’ age ranged 4 to 60...

The acquired resistance of gastrointestinal stromal tumors (GISTs) to the targeted-based therapy remains driving force identify novel approaches that are capable increasing sensitivity GISTs current therapeutic regimens. Our present data show BGJ398, a selective fibroblast growth factor receptor (FGFR) inhibitor, sensitizes imatinib (IM)-resistant GIST cells with tyrosine kinase (RTK) switch (loss c-KIT/gain pFGFR2a) low doses topoisomerase II inhibitors - doxorubicin (Dox) and etoposide...

Because of the problem to evaluate biological activity in water-soluble substances all phases preclinical and clinical studies, research work enabled develop original solvent for poorly soluble compounds based on parenteral nutrition. The main aim is examine impact nutrition systems exemplified by hemostatic system, characterized sensitivity variability effects response any impact, its comparison with solvents that are conventional pharmacological research. Experimental performed according...

Aim: To establish a p53-negative osteosarcoma (OS) SaOS-2 cellular subline exhibiting resistance to specific chemotherapeutic agents, including topoisomerase II inhibitors, taxanes, and vinca alkaloids. Methods: The OS the agents indicated above was generated by stepwise treatment of parental cell line with increasing concentrations doxorubicin (Dox) for 5 months. Half-inhibitory (IC50) Dox, vinblastine (Vin), paclitaxel (PTX) were calculated colorimetric MTS-based assay. Crystal violet...

Aim. To evaluate the serum level of pathogenic circulating immune complexes in patients with mild and severe atopic bronchial asthma. Methods. Serum samples asthma persistent (30 patients) (20 forms were analyzed. The control group consisted 15 healthy volunteers. detect giant, large, medium small-sized complexes, 3, 3.5, 4 7% polyethyleneglycol-6000 solutions used. For quantitative evaluation we measured ultraviolet optical density at 280 nm wave length. separate from immunoglobulin,...

Aim. To assess the potential fibrogenic risk of real occupational exposure to multi-walled carbon nanotubes (MWCNT). Methods. The study was conducted at 2 MWCNT-producing enterprises with same reactor type. 11 employees who had more than 1 year-long contact MWCNT aerosol were included in group, control group consisted 14 people. Elemental level and presence evaluated workplace air samples by transmission electron microscopy. Blood induced sputum obtained from employees, transforming growth...

Aim. To evaluate clinical value of trombodynamics in diagnosing the state coagulation way hemostasis patients with pre-eclampsia whom caesarean section was performed.Methods. The study included 66 pregnant women. group 34 them moderate to severe pre-eclampsia. During postoperative period all puerperae received low molecular weight heparin (Dalteparin 2500 IU subcutaneously once a day for 7 days). comparison consisted 32 All had scheduled Caesarean section. LMWH not administered this group....

Abstract. Introduction. Preeclampsia is a pregnancy complication characterized by the development of arterial hypertension after 20 weeks gestation, proteinuria, and/or oedemas. The pathogenesis preeclampsia remains unclear to this day. Some studies have shown importance microvesicles in various pathological conditions. Involvement relevant issue. Taking into account all above, we made an attempt consider from perspective clinical and laboratory data. Aim. To establish circulating peripheral...

Relevance. Studying features of innate and adaptive mechanisms immune response in medical workers (MW), the most vulnerable social group with a high risk infection, is an urgent research task. The aim study: Comprehensive study analyzing relationships between clinically significant polymorphisms (SNPs) TLR2, TLR4 genes, TLR2 expression level on peripheral blood monocytes, cytokine profile (IL-1β, IL-10, IL-6, IFNγ, platelet activation marker) SARS-CoV-2-specific humoral (MW) at temporary...

BACKGROUND: Coagulopathies in COVID-19 are an important aspect the pathophysiological mechanisms, clinical picture of disease, and occurrence delayed complications. AIM: To study plasma hemostasis using turbidimetry, thromboelastography, role microvesicles coagulation process patients with COVID-19. MATERIAL AND METHODS: The used blood samples from temporary infectious diseases hospital based on State Autonomous Healthcare Institution “Republican Clinical Hospital Ministry Health Republic...

Aim. To provide clinical and laboratory characteristics of anemia chronic disease in HIV infection order to improve its diagnosis. Methods. The study included 63 HIV-infected patients with disease. Assessed were the frequency complaints physical changes, erythrocyte indices, serum iron level, total iron-binding capacity blood, soluble transferrin receptor erythropoietin markers inflammation (ferritin, interleukin-1β, tumor necrosis factor-α, CD14, C-reactive protein). Comparison group...

Introduction . Mitotic poisoning agents (MPAs) affecting the dynamic state of microtubules, are well-known and effective chemotherapeutic agents. binding to thereby interfere with tubulin polymerization or depolymerization state, resulting in cell cycle arrest M-phase (mitotic catastrophe) subsequent apoptotic death. We reported previously about potent cytotoxic activities against pyrrole-carboxamides (PCs) (PC-61 PC-84) broad spectrum cancer lines, including triple negative breast cancer,...

Drug addiction is a current serious medical and social problem. The continuing all over the world during last several decades tendency of narcotic drug use growth has leaded to significant increase in number patients with abuse associated internal diseases. A huge diseases have particular features clinical course addicts chronic intoxication compared without it, which related primarily disorders addicts’ immune system, i.e. marked immunodeficiency. Immunotropic effects were still are closely...

The article presents our own clinical observations of cases abnormal uterine bleeding in postmenopausal women, where the main cause was pathology hemostasis: a patient after liver transplantation for cirrhosis with chronic viral hepatitis C, and primary idiopathic thrombocytopenic purpura which manifested at later age. Among most common causes women are hyperplasia endometrial cancer, leiomyoma, adenomyosis distinguished. Pathology hemostasis system as bleeding, prevails pubertal...

It is known that during pregnancy, the hemostatic system, like rest of body's systems, undergoes certain changes: there a gradual increase in activity coagulation unit hemostasis reaching maximum before childbirth. However, if pregnancy complicated by various hypertensive conditions, such as preeclampsia, an imbalance observed hemostasis: against background link, depletion anticoagulation factors and dysfunction fibrinolytic system are observed. All these changes create pathogenetic basis...

Aim. To study the immunostimulating effect of glucosaminyl muramyl dipeptide in treatment chronic catarrhal gingivitis patients undergoing orthodontic using fixed appliances. Methods. The data clinical and laboratory examination oral health status 54 aged 14-24 years with inflammatory periodontal diseases, appliances were analyzed. first group included 26 whom (1 mg sublingual tablets daily for 10 days) was additionally administered. second 28 receiving standard treatment. efficacy...