A5000702841- Hepatitis C virus research
- Hepatitis B Virus Studies
- Liver Disease Diagnosis and Treatment
- Monoclonal and Polyclonal Antibodies Research
- Research on Leishmaniasis Studies
- Viral gastroenteritis research and epidemiology
- Viral Infections and Immunology Research
- Animal Virus Infections Studies
- RNA and protein synthesis mechanisms
- Virus-based gene therapy research
- vaccines and immunoinformatics approaches
- Trypanosoma species research and implications
- Immunotherapy and Immune Responses
- Influenza Virus Research Studies
- Salmonella and Campylobacter epidemiology
- Blood groups and transfusion
- Liver Disease and Transplantation
- Identification and Quantification in Food
- Protein purification and stability
- Parvovirus B19 Infection Studies
- Cervical Cancer and HPV Research
- Bacteriophages and microbial interactions
- Immune Cell Function and Interaction
- Food Safety and Hygiene
- Glycosylation and Glycoproteins Research
University of Tehran
2011-2025
Tehran University of Medical Sciences
2013-2024
Swedish Institute
2004
Abstract Hepatitis C virus (HCV) subtypes were determined in 125 Iranian patients by phylogenetic analysis within the NS5B or 5′‐UTR/core regions. Subtypes 1a and 3a predominant accounting for 47 36%, whereas 1b 4 accounted 8 7%. This subtype distribution differs from that of Turkey Pakistan, where dominate also neighbouring Arabic countries is prevalent genotype. The strains formed subclusters dendrogram indicating these are indigenous to Iran. In contrast, intermixed with derived...
Background: Food-borne diseases are one of the major problems in developing countries. This study aims to investigate prevalence, antibiotic resistance pattern, and serotyping Shigella isolates from Olivier salad. Methods: 150 samples salad, including 50 industrial 10 different available brands Tehran's shops 100 traditional southern regions, were randomly obtained between April October 2021. These examined for bacterial isolation identification, which was finally confirmed by API-20E kit....
Chronic hepatitis C virus infection is a major cause of mortality due to liver cirrhosis globally. Despite the advances in recent therapeutic strategies, there yet high burden HCV-related worldwide concerning low coverage newly developed antiviral therapies, insufficient validity current diagnostic methods for cirrhosis, and incomplete understanding pathogenesis this stage disease. Hence we aimed clarify molecular events identify liver-specific gene signature potentially improve diagnosis...
Hepatitis C is prevalent among thalassemia patients in Iran. It mainly transfusion mediated, particular treated before 1996 when blood screening was introduced.The current study aimed to investigate why still seroconvert anti-HCV Iranian centers.During 2006-2007 sera were sampled from 217 positive at nine centers Tehran and Amol city, where 34 (16%) had been infected after 1996. The HCV subtype could be determined by sequencing phylogenetic analysis of partial NS5B and/or 5׳NCR-core region...
There is no published data on association of HLA class II alleles with clearance or persistence after acute hepatitis C virus (HCV) infection in patients from Iran. DRB1, DQA1, and DQB1 were determined using polymerase chain reaction amplification sequence specific primers (PCR-SSP) a total 117 thalassemia (63 chronic infection, 54 viral clearance) 120 healthy controls. HLA-DRB1*0301 DQA1*0501 found significantly present HCV compared to those (P = 0.03 P 0.0007, respectively). By contrast,...
Feeble cellular responses induced by T cell-based vaccines are a major challenge for the development of an effective vaccine against Hepatitis C virus (HCV) infection. To address this challenge, potential N-terminal fragment gp96 heat shock protein (rNT (gp96) as adjuvant was evaluated and compared to that CpG (as recognized Th1-type adjuvant) in formulation HCV core/NS3 antigens three immunization strategies protein/protein, DNA/DNA, DNA/protein. Immunized mice were elicited immune week 3...
Detection of Leishmania RNA virus (LRV) in Old World species and their possible role the disease prognosis requires sensitive specific methods, preferably independent viral genome. We aimed to develop an indirect immunofluorescence antibody (IFA) assay detect LRV parasites.Clinical samples were collected from 86 cutaneous leishmaniasis (CL) patients different endemic areas CL Iran, during 2017-2019. For preparation, viruses obtained sediment LRV-infected L. major culture-using freeze thaw...
Background: Despite the success of "direct-acting antivirals" in treating Hepatitis C Virus (HCV) infection, invention a preventive HCV vaccine is crucial for global elimination virus. Recent data indicated importance induction Pan-genomic neutralizing Antibodies (PnAbs) against heterogenic Envelope 2(E2), cellular receptor binding antigen, by any candidate. To overcome HCVE2 heterogeneity, "generation consensus sequences" proposed. However, Consensus Sequence (CS) generating algorithms such...
Abstract Background Despite recent advancements, limitations in the treatment and control of hepatitis C virus (HCV) infection reprioritized studies for invention an efficient HCV vaccine to elicit strong neutralizing antibodies (NAbs) cellular responses. Methods Herein, we report molecular construction a BacMam virus-based surface display subtype-1a gpE2 (Bac-CMV-E2-gp64; Bac) that both expressed displayed mammalian cells bacouloviral envelope, respectively. Results Assessments by western...
Background: Pumilio/fem-3 mRNA binding factor (PUF) proteins can bind RNA in a sequence-specific manner. The deciphered RNA-recognition code of these has enabled researchers to design engineered PUF proteins, capable any desired target order modify its ultimate fate. In this study, modified Homo sapiens Pumilio 1-homology domain (HsPUM1-HD) was the internal ribosome entry site (IRES) hepatitis C virus (HCV) genome potentially inhibit viral translation. Methods: Based on code, required...
Background: Development of an effective prophylactic vaccine is the optimal long-term goal for eventual control HCV infection. An should be able to elicit neutralizing antibodies (NAbs). Glycoprotein E2 major target NAbs. Methods: In this study, we designed and constructed a DNA (pcDNA-E2-NT(gp96)) encoding fusion protein composed ectodomain (genotype 1a) N-terminal domain gp96 as biological adjuvant. Two possible forms protein, namely E2-NT(gp96) NT(gp96)-E2, were made subjected in silico...