A5024758067- Rabies epidemiology and control
- Virology and Viral Diseases
- Bacillus and Francisella bacterial research
- Viral Infections and Outbreaks Research
- Virus-based gene therapy research
- Bacteriophages and microbial interactions
- SARS-CoV-2 and COVID-19 Research
- Poxvirus research and outbreaks
- Nuclear Engineering Thermal-Hydraulics
- Heat Transfer and Boiling Studies
- Heat transfer and supercritical fluids
- Immunotherapy and Immune Responses
- Nuclear reactor physics and engineering
- Viral gastroenteritis research and epidemiology
- Microbial infections and disease research
- Animal Virus Infections Studies
- Influenza Virus Research Studies
- Immune responses and vaccinations
- Fluid Dynamics and Mixing
- Analytical chemistry methods development
- Nuclear Physics and Applications
- Blood groups and transfusion
- Venomous Animal Envenomation and Studies
- COVID-19 Clinical Research Studies
- Yersinia bacterium, plague, ectoparasites research
National Institutes for Food and Drug Control
2014-2025
Northwest Institute of Nuclear Technology
2022-2024
Beijing Administration Institute
2013
Ministero della Salute
2013
Rabies is a serious public health problem worldwide for which an effective treatment method lacking but can be prevented by vaccines. Current vaccines are produced in cell or egg cultures, both costly and time consuming.Here, non-replicating mRNA vaccine (RV021) encoding the rabies virus glycoprotein was developed vitro, its immunogenicity protective efficacy against live evaluated mice.A two-dose vaccination with 1 μg of RV021 at 7-day intervals induced level neutralizing antibody that...
Abstract The emerging of emergent SARS‐CoV‐2 subvariants has reduced the protective efficacy COVID‐19 vaccines. Therefore, novel vaccines targeting these variants are needed. We designed and prepared CoV072, an mRNA‐based vaccine against Omicron (EG.5) other that encodes EG.5 spike protein. Six‐week‐old female BALB/C mice were used to assess humoral cellular immune responses cross‐reactive neutralizing activity various subvariants. Meanwhile different immunization strategies doses performed...
Host cell proteins (HCPs) from primary hamster kidney cells (PHKCs) used to produce rabies vaccines may cause an allergic reaction in humans, so these residual HCPs must be controlled. Establishing a national standard for PHKC HCP is very important ensure the consistency of between batches vaccine and standardize control HCPs. We aimed establish novel substance determine residue produced with PHKCs. A two-step multi-laboratory collaborative collaboration was undertaken. In first step,...
ABSTRACT The efficacy of the G-protein is influenced by N-linked glycosylation, which serves as sole immunogen rabies virus vaccine. However, achieving satisfactory immune-protection remains challenging, owing to heterogeneous glycosylation G-proteins. Within molecular dynamics, examining impact N-glycan heterogeneity on structural characteristics G-proteins provides insights into relationship between antigens and vaccines. Glycosylation regulated host cells. In cultured in Vero cells (VRV),...
The efficacy of many coronavirus disease 2019 (COVID-19) vaccines has been shown to decrease varying extents against new severe acute respiratory syndrome 2 variants, which are responsible for the continuing COVID-19 pandemic. Combining intramuscular and intranasal vaccination routes is a promising approach achieving more potent immune responses. We evaluated immunogenicity prime-boost protocols with chimpanzee adenovirus serotype 68 vector-based vaccine, ChAdTS-S, administered via both in...
We evaluated the immunogenicity persistence of different immunization regimens freeze-dried human rabies vaccine (Vero cells) in 10–60 years age group Chinese population. Based on phase III clinical trials Vero cells, participants who completed full course were continuously enrolled four-dose trial, five-dose, and five-dose control groups. Venous blood samples collected 12 months after completion for virus-neutralizing antibody (RVNA) testing, antibody-positive rate geometric mean...
Background: The Vero cell rabies vaccine is currently the most widely used human vaccine. However, owing to presence of residual host DNA (HCD) in final product and potential tumorigenicity high-passage cells, WHO not only sets a limit on number times cells production can be passaged, but also imposes strict requirements amount HCD product. Objectives: To systematically reduce level product, multiple purification steps are included process. This study investigated effectiveness key antigen...
This collaborative study developed a Vero cell DNA reference for standardizing dot blot hybridization, an assay widely employed to measure residual contents of viral vaccines prepared with cells. High purity was extracted and characterized by Hind III enzyme digestion sequencing. Then, cooperative calibration, the concentration bulk solution determined (64.0 ± 1.9 μg/mL, OD260/OD280 = 1.87) diluted (40 ng/mL) Tris-EDTA buffer containing bovine serum albumin as freeze-dried excipients. With...
The ongoing evolution of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2 or 2019-nCoV) variants has been associated with the transmission and pathogenicity COVID-19. Therefore, exploring optimal immunisation strategy to improve broad-spectrum cross-protection ability COVID-19 vaccines is great significance. Herein, we assessed different heterologous prime-boost strategies chimpanzee adenovirus vector-based plus Wuhan-Hu-1 (WH-1) strain (AdW) Beta variant (AdB) mRNA-based WH-1...
The large-scale production of a human diploid cell (HDC) vaccine (HDCV) for rabies is limited by several technical challenges. Kanghua Biological Products Co., Ltd., has successfully used microcarrier technology the culture HDCs in bioreactors to develop lyophilized and purified HDCV. In this blinded, randomized, parallel-group study conducted between July October 2014 Mianzhu, Sichuan Province, China, we monitored safety immunogenicity healthy population vaccinated according Essen...
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The aim of this study is to demonstrate that the freeze-dried human rabies vaccine (Vero cell), administered in a four-dose schedule (2-1-1) 10–60 years old population, has immunogenicity not inferior approved five-dose and similar vaccines with schedule, evaluate its safety. A total 1800 individuals were enrolled divided into three groups: test group, control group. virus neutralizing antibodies measured using Rapid Fluorescent Focus Inhibition Test assess immunogenicity, incidence adverse...
This study evaluated the 12-month persistence of rabies virus-neutralizing antibody (RVNA) following different immunization regimens freeze-dried human vaccine (Vero cells) in individuals aged 10-60 years within Chinese population.
Recently optimized freeze-dried rabies vaccines (Vero cell) are promising for prevention; however, comparisons of Essen 5-dose and Zagreb 4-dose regimens needed clinical applications. This phase III trial aimed to assess the safety immunogenicity candidate developed human use by Shandong Yeedo Biotechnology (Registration No.: CTR20182016, http://www.chinadrugtrials.org.cn/index.html). In total, 40 participants in stage I 1797 subjects II with an age range 10–60 years were recruited. Both...
Objective To analyze the current epidemiological characteristics of rabies in China, grasp law epidemic changes, and provide scientific basis for prevention control strategies China. Methods The data human China were systematically collected, combined with genetic sequences virus street strains various provinces statistical molecular biological methods used statistics analysis. Results number cases deaths has decreased year by year. At present, main method transmission is dog injury....
<p><strong>Objective</strong><strong>：</strong>To validate the relativity between rapid fluorescent focus inhibition tests (RFFIT) and mouse neutralization test (MNT), In order to replace potency of anti-rabies serum/ immunoglobulin. <strong>Method: </strong>The sample reference standard were diluted 3-fold serially in 96-well microplate, then mix with a certain amount challenge virus causing infection 80%~95% cells. Incubated at 37 for 1hr vitro,...