A5054512724- Endodontics and Root Canal Treatments
- Mesenchymal stem cell research
- MicroRNA in disease regulation
- Cancer, Hypoxia, and Metabolism
- Immune Response and Inflammation
- Cancer-related molecular mechanisms research
- NF-κB Signaling Pathways
- Dental Research and COVID-19
- Dental Radiography and Imaging
- Proteoglycans and glycosaminoglycans research
- Aquaculture disease management and microbiota
- Oral and Maxillofacial Pathology
- Ion Channels and Receptors
- Legionella and Acanthamoeba research
- Angiogenesis and VEGF in Cancer
- Oral microbiology and periodontitis research
- Bone Metabolism and Diseases
- Inflammatory mediators and NSAID effects
- Signaling Pathways in Disease
- Wound Healing and Treatments
- Dental materials and restorations
- Physiological and biochemical adaptations
- Hemostasis and retained surgical items
- Bone and Dental Protein Studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
Tokyo Medical and Dental University
2016-2025
The University of Tokyo
2016-2024
University of Michigan
2022-2023
MicroRNAs (miRNAs) play a crucial role in cell differentiation through epigenetic regulation of gene expression. In human dental pulp cells, we have identified miRNA-27a being upregulated under inflammatory conditions. Here, aimed to examine whether (i) overexpression stem cells (hDPSCs) enhances their odonto/osteoblastic via Wnt and bone morphogenetic protein signaling; (ii) hDPSCs overexpressing promote new formation vivo. were cultured osteogenic medium differentiation. To the miRNA-27a,...
microRNAs are small noncoding RNA molecules that regulate silencing and posttranscriptional gene expression, many involved in inflammatory processes. In particular, microRNA 21 (miR-21) is upregulated environment reported to induce anti-inflammatory responses. However, the involvement of miR-21 pulpal inflammation precise mechanisms reactions induced by remain unclear. We hypothesized miR-21-5p expression lipopolysaccharide (LPS)-stimulated human dental pulp cells (hDPCs) downregulates...
Abstract Tissue-resident macrophages expressing lymphatic vessel endothelial hyaluronan receptor-1 (LYVE-1) are found in multiple tissues and organs. We aimed to evaluate the dynamics biological functions of LYVE-1 + dental pulp during post-injury tissue remodeling. Immunofluorescence staining mouse embryos revealed that colonized before birth. In mature rat molar pulp, were main subset CD163, an M2 marker, distributed throughout tissue. response injury induced by cavity preparation, quickly...
Abstract Accelerated dental pulp mineralization is a common complication in avulsed/luxated teeth, although the mechanisms underlying this remain unclear. We hypothesized that hypoxia due to vascular severance may induce osteo/odontoblast differentiation of stem cells (DPSCs). This study examined role B-cell CLL/lymphoma 9 (BCL9), which downstream hypoxia-inducible factor 1α (HIF1α) and Wnt/β-catenin transcriptional cofactor, osteo/odontoblastic human DPSCs (hDPSCs) under hypoxic conditions....
MicroRNA-27a-5p (miR-27a-5p) was significantly upregulated in dental pulp inflammation, yet its underlying mechanisms remain unclear. This study investigated the effect of miR-27a-5p on expression proinflammatory cytokines human cells (hDPCs) stimulated by lipopolysaccharide (LPS). LPS-stimulated hDPCs showed concurrent increases and (IL-6, IL-8, MCP1), increased suppressed NF-κB inhibitor BAY 11-0785. Transfection mimic downregulated cytokines, activity, signaling activators (TAB1, IRAK4,...
Abstract Background Droplets and aerosols produced during dental procedures are a risk factor for microbial viral transmission. Unlike sodium hypochlorite, hypochlorous acid (HOCl) is nontoxic to tissues but still exhibits broad microbicidal effect. HOCl solution may be applicable as supplement water and/or mouthwash. This study aims evaluate the effectiveness of on common human oral pathogens SARS-CoV-2 surrogate MHV A59 virus, considering practice environment. Methods was generated by...
This study examined the effects and mechanisms of strontium ranelate (SrRn)-a drug used to treat osteoporosis-on proliferation differentiation/mineralization cloned dental pulp-like cells (mouse papillae cells; MDPs). It also determined whether topical application SrRn exposed pulp tissue promotes formation mineralized in vivo. The MDPs were cultured with or without SrRn, cell proliferation, odonto-/osteoblastic gene expression, nodule formation, Akt phosphorylation evaluated. SrRn-treated...
Apical periodontitis (AP) develops as a result of an immune response to pulpal bacterial infection, and various cytokines are involved in the pathogenesis AP, with Interleukin (IL)-1 being considered key cytokine. The role IL-1 AP has been well studied. It is known that expression periapical lesions correlates closely development AP. potent bone-resorptive cytokine induces osteoclast formation activation. Hence, inhibiting its signaling receptor antagonist (IL-1RA) results reduction lesion...
Premixed calcium silicate-based materials have recently been developed and are recommended for a wide range of endodontic procedures, including vital pulp therapy. This study investigated the in vitro biocompatibility pro-mineralization effect vivo reparative dentin formation EndoSequence Root Repair Material, BCRRM, Bio-C Repair, Well-pulp PT. Both fresh set extracts had no detrimental on growth human dental stem cells. The higher concentration than induced considerably greater mineralized...
To elucidate the role of HIF1α in pro-inflammatory cytokine mRNA expression from lipopolysaccharide (LPS)-stimulated human dental pulp cells (hDPCs).mRNA interleukin (IL) 1β and tumour necrosis factor (TNF) α LPS-stimulated hDPCs was determined by quantitative RT-PCR. Expression nuclear kappa B (NFκB) p65 phospho-NFκB analysed Western blotting. Activation NFκB signalling measured luciferase assay using a reporter vector containing an response element. Enforced induced transfection vectors...
Abstract Aim To elucidate mechanisms by which mineral trioxide aggregate (MTA) suppresses pro‐inflammatory cytokine mRNA expression in lipopolysaccharide (LPS)‐stimulated RAW264.7 macrophages. Methodology Mineral extracts were prepared immersing set ProRoot MTA culture medium. cells cultured the presence of LPS and extracts. levels interleukin ( IL ) ‐1α , IL‐6 early growth response 2 Egr2 ), suppressor signalling 3 Socs3 IL‐10 quantified with reverse transcription‐quantitative polymerase...
Abstract Aim To investigate the effect of microRNA-27a-5p (miR-27a-5p) on expression proinflammatory cytokines in human dental pulp cells (hDPCs) stimulated by lipopolysaccharide (LPS). Methodology Expression miR-27a-5p was evaluated LPS-stimulated hDPCs isolated from third molars healthy patients a TaqMan microRNA assay. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and cytometric bead array were used to measure mRNA protein levels, respectively, interleukin (IL)-6,...
Apical periodontitis is an inflammatory disease involving lesions located within the jawbone. Histological evaluations generally require decalcification and sectioning, which has limited our understanding of three-dimensional (3D) organization spatial distribution different immune cell types in these lesions. A recently developed technique combining tissue clearing whole-mount immunofluorescent labeling allows us to acquire such information from deep without sectioning. However, jawbone...
Abstract Tissue-resident macrophages expressing lymphatic vessel endothelial hyaluronan receptor-1 (LYVE-1) are found in multiple tissues and organs. We aimed to evaluate the dynamics biological function of LYVE-1 + dental pulp during post-injury tissue remodeling. Immunofluorescence staining mouse embryos revealed that colonized before birth. In mature rat molar pulp, were main subset CD163, an M2 marker, distributed throughout tissue. response injury induced by cavity preparation, quickly...
Abstract Background Droplets and aerosols produced during dental procedures are a risk factor for microbial viral transmission. Unlike sodium hypochlorite, hypochlorous acid (HOCl) is nontoxic to tissues but still exhibits broad microbicidal effect. HOCl solution may be applicable as supplement water and/or mouthwash in practice. This study aims evaluate the effectiveness of on common human oral pathogens SARS-CoV-2 surrogate MHV-A59 virus, considering practice environment. Methods was...