A5068446758- Endodontics and Root Canal Treatments
- Mesenchymal stem cell research
- Dental Radiography and Imaging
- Bone and Dental Protein Studies
- Cancer, Hypoxia, and Metabolism
- Oral and Maxillofacial Pathology
- Bone Metabolism and Diseases
- Dental materials and restorations
- Angiogenesis and VEGF in Cancer
- Electrospun Nanofibers in Biomedical Applications
- MicroRNA in disease regulation
- Immune cells in cancer
- Periodontal Regeneration and Treatments
- Bone Tissue Engineering Materials
- Tissue Engineering and Regenerative Medicine
- Cancer-related molecular mechanisms research
- dental development and anomalies
- Ion Channels and Receptors
- Proteoglycans and glycosaminoglycans research
- Hemostasis and retained surgical items
- Physiological and biochemical adaptations
- Inflammatory mediators and NSAID effects
- Aquaculture disease management and microbiota
- Immune Response and Inflammation
- Wound Healing and Treatments
Tokyo Medical and Dental University
2017-2025
The University of Tokyo
2017-2022
MicroRNAs (miRNAs) play a crucial role in cell differentiation through epigenetic regulation of gene expression. In human dental pulp cells, we have identified miRNA-27a being upregulated under inflammatory conditions. Here, aimed to examine whether (i) overexpression stem cells (hDPSCs) enhances their odonto/osteoblastic via Wnt and bone morphogenetic protein signaling; (ii) hDPSCs overexpressing promote new formation vivo. were cultured osteogenic medium differentiation. To the miRNA-27a,...
Dental pulp stem cells (DPSCs) are a good source for tissue regeneration, however, the number of DPSCs in is limited. Cell propagation essential engineering using and cell culture conditions may affect properties DPSCs. The purpose this study was to analyze effect condition, especially dense on property differentiation pathway We cultured under sparse (sDPSCs; 5 × 103 cells/cm2) or (dDPSCs; 1 105 4 days compared their properties. populations CD73+ CD105+ were significantly decreased dDPSCs....
microRNAs are small noncoding RNA molecules that regulate silencing and posttranscriptional gene expression, many involved in inflammatory processes. In particular, microRNA 21 (miR-21) is upregulated environment reported to induce anti-inflammatory responses. However, the involvement of miR-21 pulpal inflammation precise mechanisms reactions induced by remain unclear. We hypothesized miR-21-5p expression lipopolysaccharide (LPS)-stimulated human dental pulp cells (hDPCs) downregulates...
Abstract Tissue-resident macrophages expressing lymphatic vessel endothelial hyaluronan receptor-1 (LYVE-1) are found in multiple tissues and organs. We aimed to evaluate the dynamics biological functions of LYVE-1 + dental pulp during post-injury tissue remodeling. Immunofluorescence staining mouse embryos revealed that colonized before birth. In mature rat molar pulp, were main subset CD163, an M2 marker, distributed throughout tissue. response injury induced by cavity preparation, quickly...
Abstract Accelerated dental pulp mineralization is a common complication in avulsed/luxated teeth, although the mechanisms underlying this remain unclear. We hypothesized that hypoxia due to vascular severance may induce osteo/odontoblast differentiation of stem cells (DPSCs). This study examined role B-cell CLL/lymphoma 9 (BCL9), which downstream hypoxia-inducible factor 1α (HIF1α) and Wnt/β-catenin transcriptional cofactor, osteo/odontoblastic human DPSCs (hDPSCs) under hypoxic conditions....
The mineralized tissue-inductive ability and anti-inflammatory properties of hydraulic calcium silicate-based (HCSB) sealers have not been fully elucidated. This study aimed to evaluate the effects HCSB Bio-C sealer (BioC), Well-Root ST (WST), EndoSequence BC (BC), on osteoblastic differentiation/mineralization proinflammatory cytokine synthesis by macrophages.Diluted extracts set or chloride solutions approximately equivalent Ca2+ concentrations were applied a mouse cell line (Kusa-A1...
This study examined the effects and mechanisms of strontium ranelate (SrRn)-a drug used to treat osteoporosis-on proliferation differentiation/mineralization cloned dental pulp-like cells (mouse papillae cells; MDPs). It also determined whether topical application SrRn exposed pulp tissue promotes formation mineralized in vivo. The MDPs were cultured with or without SrRn, cell proliferation, odonto-/osteoblastic gene expression, nodule formation, Akt phosphorylation evaluated. SrRn-treated...
Premixed calcium silicate-based materials have recently been developed and are recommended for a wide range of endodontic procedures, including vital pulp therapy. This study investigated the in vitro biocompatibility pro-mineralization effect vivo reparative dentin formation EndoSequence Root Repair Material, BCRRM, Bio-C Repair, Well-pulp PT. Both fresh set extracts had no detrimental on growth human dental stem cells. The higher concentration than induced considerably greater mineralized...
To elucidate the role of HIF1α in pro-inflammatory cytokine mRNA expression from lipopolysaccharide (LPS)-stimulated human dental pulp cells (hDPCs).mRNA interleukin (IL) 1β and tumour necrosis factor (TNF) α LPS-stimulated hDPCs was determined by quantitative RT-PCR. Expression nuclear kappa B (NFκB) p65 phospho-NFκB analysed Western blotting. Activation NFκB signalling measured luciferase assay using a reporter vector containing an response element. Enforced induced transfection vectors...
Abstract Aim To elucidate mechanisms by which mineral trioxide aggregate (MTA) suppresses pro‐inflammatory cytokine mRNA expression in lipopolysaccharide (LPS)‐stimulated RAW264.7 macrophages. Methodology Mineral extracts were prepared immersing set ProRoot MTA culture medium. cells cultured the presence of LPS and extracts. levels interleukin ( IL ) ‐1α , IL‐6 early growth response 2 Egr2 ), suppressor signalling 3 Socs3 IL‐10 quantified with reverse transcription‐quantitative polymerase...
A prototype surface-reaction-type pre-reacted glass-ionomer (S-PRG) filler containing root canal sealer (S-PRG sealer) exhibits bioactive potential by releasing multiple ions. This study explored the suppressive effects and modes of action S-PRG extracts on proinflammatory cytokine expression in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. Expression cytokines was evaluated RT-qPCR ELISA. phosphorylated nuclear factor-kappa B (p-NF-kB) p65 western blotting. significantly downregulated...
Abstract Tissue-resident macrophages expressing lymphatic vessel endothelial hyaluronan receptor-1 (LYVE-1) are found in multiple tissues and organs. We aimed to evaluate the dynamics biological function of LYVE-1 + dental pulp during post-injury tissue remodeling. Immunofluorescence staining mouse embryos revealed that colonized before birth. In mature rat molar pulp, were main subset CD163, an M2 marker, distributed throughout tissue. response injury induced by cavity preparation, quickly...
Background/purpose: Vascular endothelial growth factor A (VEGFA) is a potent angiogenic and an essential for vascular cells, but its effects on dental pulp stem such as cells from human exfoliated deciduous teeth (SHEDs), have not been fully evaluated. The aim of this study was to explore the underlying mechanisms VEGFA odonto/osteoblastic differentiation in SHEDs vitro. This also aimed examine mineralized tissue-forming pro-angiogenic potentials rat vivo. Proliferation, migration, gene...