Autophagy is a biological process characterized by self-digestion and involves induction of autophagosome formation, leading to degradation autophagic cargo. Aging associated with the reduction autophagy activity neurodegenerative disorders, chronic inflammation, susceptibility infection; however, underlying mechanism unclear. DNA methylation methyltransferases reduces expression corresponding genes. Since macrophages are major players in inflammation defense against infection we determined...

Amongst the most severe clinical outcomes of life-long infections with Helicobacter pylori is development peptic ulcers and gastric adenocarcinoma - diseases often associated an increase regulatory T cells. Understanding H. pylori-driven regulation cells therefore crucial importance. Several studies have defined mammalian microRNAs as key regulators immune system carcinogenic processes. Hence, we aimed here to identify pylori-regulated miRNAs, mainly in human MicroRNA profiling non-infected...

Cystic fibrosis (CF) is a fatal, genetic disorder that critically affects the lungs and directly caused by mutations in CF transmembrane conductance regulator (CFTR) gene, resulting defective CFTR function. Macroautophagy/autophagy highly regulated biological process provides energy during periods of stress starvation. Autophagy clears pathogens dysfunctional protein aggregates within macrophages. However, this impaired patients mice, as their macrophages exhibit limited autophagy activity....

Introduction: Hymenosporum flavum (Hook.) F. Muell. is the sole species within genus known for its antimicrobial activity. The current study aims to examine prospective activity of H. as a safe supporter sorafenib (as reference standard) against hepatocellular carcinoma (HCC). Methods: Isolation and identification compounds were made by chromatographic spectroscopic methods. A fingerprint plant extract was done using HPLC-MS/MS spectrometric analysis. total examined in vitro HCC isolated...

The antiviral response against influenza A virus (IAV) infection includes the induction of interferon (IFN) signaling pathway, including activation STATs protein family. Subsequently, myxovirus resistance (MxA) and other interferon-stimulated genes control replication; however, molecular interaction viral-mediated IFN needs more investigation. Host microRNAs (miRNAs) are small non-coding molecules that posttranscriptionally regulate gene expression. Here, we sought to investigate possible...

Aim suppression of methylation inhibitors (epigenetic genes) in hepatocarcinogenesis induced by diethylnitrosamine using glycyrrhetinic acid. Method In the current work, we investigated effect sole GA combined with different agents such as doxorubicin (DOX) or probiotic bacteria ( Lactobacillus rhamanosus ) against to improve efficiency. The genomic DNA was isolated from rats’ liver tissues evaluate either methylation-sensitive methylation-dependent resection enzymes. activity targeting...

Microtubule-associated protein 1 (MAP1) light chain 3 (LC3) has proven useful as autophagosomal marker in studies on the interaction between pathogens and host autophagic machinery. However, function of LC3 is known to extend above beyond its role autophagosome formation. We previously reported that intrinsic associated with intracellular Chlamydia trachomatis inclusion human epithelial cells. Here we show LC3, most likely cytoplasmic nonlipidated form, interacts C. a microtubule-associated...

Influenza A virus is a negative RNA stranded of the family Orthomyxoviridae, and represents major public health threat, compounding existing disease conditions. replicates rapidly within its host segmented nature genome facilitates re-assortment, whereby whole genes are exchanged between influenza subtypes during replication. Antiviral medications important pharmacological tools in prophylaxis therapy. However, use currently available antiviral impeded by sometimes high levels resistance...

Therapeutic selectivity is a critical issue in cancer therapy. As result of its adjustable physicochemical characteristics, the Au/cellulose nanocomposite currently holds lot potential for solving this challenge. This work was designed to prepare with enhanced anticancer activity through regulation mitogen-activated protein kinases (MAPK) signaling pathway. Nanocellulose, nanogold (AuNPs), and were biosynthesized from microgreen alga Chlorella vulgaris. Using UV-Vis absorption spectroscopy,...

Breast cancer is one of the most common causes death in women worldwide and has harmful influence on their psychological state during therapy. Multikinase inhibitors have become effective drugs for treating a variety diseases such as breast cancer. A purified short peptide (H-P) was isolated from natural honey tested its potential regulatory role cells compared with effectiveness anticancer drug, Sorafenib (SOR), using MCF-7, EFM-19, MCF-10A cell lines. Furthermore, we investigated direct...

Influenza is a highly infectious disease caused by three types of viruses, including influenza A virus (IAV), B virus, and, rarely, C virus. IAV major, global public health threat, causing approximately 500 000 deaths per year worldwide. The new strains have emerged due to mutation called antigenic shift, which results in subtype the that shows resistance common antiviral drugs. Here, guava and lemon extracts, green leaves flowers, were investigated for their activity against replication...

Background: A heart attack occurs when coronary artery blockage interrupts the blood supply to such as is seen in cardiovascular disease (CVD). Importantly, autophagy commonly regarded a host defense mechanism against microbial invaders. Methods: total of 50 samples were obtained from (CV) patients addition 30 that healthy individuals and served controls. Macrophages isolated vitro propagated samples. Autophagosome formation, cytokine secretion, apolipoprotein B (ApoB) gene expression...

Introduction: Cancer is one of the most difficult challenges faced by humanity due to its many associated issues, such as inability prevent diseases, treatment safety, and high mortality rate. In cancer, a variety cellular signaling activated ensure malignancy transformation, angiogenesis metastasis. The efficient pathway in cancer mitogen-activated protein kinase (MAPK), which controls regulates apoptosis. Methods: Four different flavonoid glycosides have been isolated from Pulicaria...

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Introduction: Hepatitis C is a liver disease caused by the hepatitis virus (HCV). It can cause both acute and chronic infection. Based on secretion of required cytokines upon infection, HCV improve its own RNA successfully complete replication cycle. Importantly, single nucleotide polymorphisms (SNPs) are most common type genetic variation have been found to play critical role in modulation cellular cytokine production interaction. Methodology: A total 100 blood samples were obtained from...

Abstract Our laboratory has shown that calpain‐2 activation in the brain following acute injury is directly related to neuronal damage and long‐term functional consequences of injury, while calpain‐1 generally neuroprotective deletion exacerbates injury. We have also a relatively selective inhibitor, referred as C2I, enhanced potentiation learning memory, provided neuroprotection controlled cortical impact (CCI) model traumatic (TBI) mice. Using molecular dynamic simulation Site...

Abstract Liver cancer is one of the most pivotal global health problems, leading hepatocellular carcinoma (HCC) with a significant increase in cases worldwide. The role non-coding-RNA proliferation and carcinogenesis has attracted much attention last decade; however, microRNAs (miRNAs), as non-coding RNA, are considered master mediators various progressions. Yet miR-141 modulator for specific cellular processes liver cell still unclear. This study identified its potential functions...

Fluvastatin (FLV) is a statin family member that may play role in modulating variety of medical disorders such as atherosclerosis and breast cancer. The present study addresses the ability FLV to modulate cellular immune response provides new nanosized formula (self-nanoemulsifying delivery system, SNED) potentially more effective for suppression cancer development. We monitored autophagic machinery through expression microtubule-associated protein 1A/1B-light chain 3 (LC3I/II). Lysosomal...

Mushrooms have become an important way to supply the body with daily needs of organic selenium safely and remarkable medicinal properties. In this study, we examined ability Pleurotus ostreatus mushroom grow in (Se) its accumulate convert Se from inorganic form into during growth. Additionally, achieved potential anticancer properties extract colon cancer cells using CaCo-2 cell normal human mucosal epithelial line, NCM-460 line. Interestingly, Se-enriched (SME) showed a competitive...

This study was conducted to investigate the potential anticancer properties of N, N-dibenzyl asparagine (NNDAsp), as an Asparagine (Asp) analog, using colon cancer Caco-2 cell and normal NCM-460 line.Cell viability rate levels produced lactate dehydrogenase (LDH) were achieved upon treatment with NNDAsp compared Asp MTT assay LDH production kit. The protein expression profile synthetase (ASNS) by ELISA flow cytometry assay. released inflammatory cytokines, including interleukin-1 alpha...

In the current work, we aimed to evaluate protective effects of liquorice and halfa-bar extract against doxorubicin (DOX)-induced nephritic syndrome (NS) in rats. Twenty albino male rats were intraperitoneally injected with 50 mg/kg DOX. The supplied daily 400 liquorice, extract, or their combination for 2 weeks. Our findings confirmed induction NS indicated by alteration Bowman's space, damaged glomerular capsules, tubules. Moreover, levels produced tumour necrosis factor-alpha (TNF-α)...

Abstract Introduction MicroRNAs (miRNAs) are single RNA molecules that act as global regulators of gene expression in mammalian cells and thus constitute attractive targets treating cancer. Here we aimed to investigate the possible involvement miRNA-141 (miR-141) cervical cancer identify its potential cell lines. Methods The level miR-141 HeLa C-33A has been assessed using quantitative real-time PCR (qRT-PCR). A new construct performed a CMV promoter vector tagged with GFP. Using microarray...