A5033971354- Virus-based gene therapy research
- Influenza Virus Research Studies
- interferon and immune responses
- Xenotransplantation and immune response
- Animal Genetics and Reproduction
- Respiratory viral infections research
- RNA and protein synthesis mechanisms
- Reproductive tract infections research
- Galectins and Cancer Biology
- Animal Virus Infections Studies
- Helicobacter pylori-related gastroenterology studies
- Protein Structure and Dynamics
- Herpesvirus Infections and Treatments
- RNA Research and Splicing
- Viral gastroenteritis research and epidemiology
- Poxvirus research and outbreaks
- RNA modifications and cancer
- Urinary Tract Infections Management
- Gut microbiota and health
- Neonatal Health and Biochemistry
- ATP Synthase and ATPases Research
- Porphyrin Metabolism and Disorders
- Tryptophan and brain disorders
- IL-33, ST2, and ILC Pathways
- Toxoplasma gondii Research Studies
Max Planck Institute for Infection Biology
2011-2023
Charité - Universitätsmedizin Berlin
2007-2019
Steinbeis Foundation
2016-2019
Max Planck Society
2008-2018
Robert Koch Institute
2003-2010
Activation of transcription factor NF-κB is a hallmark infection with the gastric pathogen Helicobacter pylori, associated inflammation and carcinogenesis. Genome-wide RNAi screening revealed numerous host factors involved in H. pylori-, but not IL-1β- TNF-α-dependent regulation. Pathway analysis including CRISPR/Cas9-knockout recombinant protein technology, immunofluorescence microscopy, immunoblotting, mass spectrometry, mutant pylori strains identified metabolite...
Many intracellular pathogens that replicate in special membrane bound compartments exploit cellular trafficking pathways by targeting small GTPases, including Rab proteins. Members of the Chlamydiaceae recruit a subset proteins to their inclusions, but significance these interactions is uncertain. Using RNA interference, we identified Rab6 and Rab11 as important regulators Chlamydia infections. Depletion either or Rab11, not other tested, decreased formation infectious particles. We further...
A growing body of evidence suggests the non-protein coding human genome is vital importance for cell function. Besides small RNAs, diverse class long non-coding RNAs (lncRNAs) recently came into focus. However, their relevance infection, a major evolutionary driving force, remains elusive. Using two commercially available microarray systems, namely NCode™ and Sureprint™ G3, we identified differential expression 42 ncRNAs during influenza virus (IAV) infection in lung epithelial cells. This...
Amongst the most severe clinical outcomes of life-long infections with Helicobacter pylori is development peptic ulcers and gastric adenocarcinoma - diseases often associated an increase regulatory T cells. Understanding H. pylori-driven regulation cells therefore crucial importance. Several studies have defined mammalian microRNAs as key regulators immune system carcinogenic processes. Hence, we aimed here to identify pylori-regulated miRNAs, mainly in human MicroRNA profiling non-infected...
Consumption of cigarette smoke (CS) is a well-known risk factor for early atherosclerosis; yet, the underlying mechanisms smoking-associated atherosclerosis are poorly understood. Based on previous results indicating that CS-induced endothelial cell death neither shows typical features apoptosis nor necrosis, we investigated role autophagy in CS extract (CSE)-induced human umbilical vein cells (HUVECs).Here, demonstrate overexpression classical inhibitor BCL-XL had no protective effect...
Abstract Chikungunya virus (CHIKV) is a globally spreading alphavirus against which there no commercially available vaccine or therapy. Here we use genome-wide siRNA screen to identify 156 proviral and 41 antiviral host factors affecting CHIKV replication. We analyse the cellular pathways in human genes are involved druggable targets. Twenty-one small-molecule inhibitors, some of FDA approved, targeting six pathways, have high activity vitro , with low toxicity. Three identified inhibitors...
Helicobacter pylori colonizes the human stomach, contributing to or causing several diseases. Translocation of CagA bacterial protein into gastric epithelial cells has been linked an increased risk peptic ulcer disease and carcinoma. Upon translocation, is tyrosine phosphorylated by Src family kinases (SFKs), which themselves become inactivated via a negative feedback loop. Here, we show that tyrosine-phosphorylated disrupts adhesion AGS extracellular matrix. Owing inactivation c-Src...
Microtubule-associated protein 1 (MAP1) light chain 3 (LC3) has proven useful as autophagosomal marker in studies on the interaction between pathogens and host autophagic machinery. However, function of LC3 is known to extend above beyond its role autophagosome formation. We previously reported that intrinsic associated with intracellular Chlamydia trachomatis inclusion human epithelial cells. Here we show LC3, most likely cytoplasmic nonlipidated form, interacts C. a microtubule-associated...
This study represents a long-term follow-up of human patients receiving pig islet xenotransplantation. Eighteen had been monitored for up to 9 years potentially xenotic viruses: endogenous retrovirus, cytomegalovirus, lymphotropic herpesvirus, and circovirus type 2. No evidence viral infection was found.
Influenza viruses (IVs) tend to rapidly develop resistance virus-directed vaccines and common antivirals targeting pathogen determinants, but novel host-directed approaches might preclude development. To identify the most promising cellular targets for a approach against influenza, we performed comparative small interfering RNA (siRNA) loss-of-function screen of IV replication in A549 cells. Analysis four different strains including highly pathogenic avian H5N1 strain, an influenza B virus...
Seasonal and pandemic influenza respiratory infections are still a major public health issue. Vaccination is the most efficient way to prevent infection. One option produce vaccines cell-culture based virus propagation. Different host cell lines, such as MDCK, Vero, AGE1.CR or PER.C6 cells have been shown be good substrate for production. With respect ease of scale-up, suspension should preferred over adherent cells. Ideally, they replicate different strains with high cell-specific yields....
Influenza A virus carries eight negative single-stranded RNAs and uses spliced mRNAs to increase the number of proteins produced from them. Several genome-wide screens for essential host factors influenza replication revealed a necessity splicing splicing-related factors, including Cdc-like kinase 1 (CLK1). This CLK family plays role in alternative regulation through phosphorylation serine-arginine rich (SR) proteins. To examine influence that modulation has on infection, we analyzed effect...
Modified vaccinia virus Ankara is a versatile vaccine vector, well suited for transgene delivery, with an excellent safety profile. However, certain transgenes render recombinant MVA (rMVA) genetically unstable, leading to the accumulation of mutated rMVA impaired expression. This represents major challenge upscaling and manufacturing vaccines. To prevent transgene-mediated negative selection, continuous avian cell line AGE1.CR pIX (CR pIX) was modified suppress expression during generation...
Chlamydia trachomatis is an obligate intracellular pathogen responsible for a high burden of human disease. Here, loss-of-function screen using set lentivirally transduced shRNAs identified 14 host cell factors that modulate C. infectivity. Notably, knockdown dynamin, GTPase, decreased Dynamin functions in multiple cytoplasmic locations, including vesicle formation at the plasma membrane and trans-Golgi network. However, its role infection remains unclear. Here we report dynamin essential...