A5100344621- Acute Myeloid Leukemia Research
- Protein Degradation and Inhibitors
- RNA Research and Splicing
- Circular RNAs in diseases
- Cancer Genomics and Diagnostics
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- CRISPR and Genetic Engineering
- Chronic Myeloid Leukemia Treatments
- Genomics and Chromatin Dynamics
- Retinoids in leukemia and cellular processes
- Mitochondrial Function and Pathology
- Neuroinflammation and Neurodegeneration Mechanisms
- Single-cell and spatial transcriptomics
- Cancer-related Molecular Pathways
- Ovarian function and disorders
- Parasites and Host Interactions
- interferon and immune responses
- Epigenetics and DNA Methylation
- Plant Molecular Biology Research
- Developmental Biology and Gene Regulation
- Reproductive Biology and Fertility
- Plant Gene Expression Analysis
- Extracellular vesicles in disease
- RNA Interference and Gene Delivery
Ruijin Hospital
2015-2025
Shanghai Jiao Tong University
2009-2025
Jiangxi Academy of Sciences
2021-2025
Fudan University
2024-2025
Capital Medical University
2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2024
Shanghai Institute of Hematology
2015-2024
Leiden University Medical Center
2024
Shanghai Ninth People's Hospital
2024
First Affiliated Hospital of Gannan Medical University
2023-2024
Circulating tumor cells (CTCs), circulating DNA (ctDNA), and extracellular vehicles (EVs) have received significant attention in recent times as emerging biomarkers subjects of transformational studies. The three main branches liquid biopsy evolved from the primary detection targets—CTC, ctDNA, EVs—each with distinct benefits. CTCs are derived cancer original or metastases may display global features tumor. ctDNA has been extensively analyzed used to aid diagnosis, treatment, prognosis...
RNA activation (RNAa) is a newly discovered mechanism of gene triggered by small double-stranded RNAs termed 'small activating RNAs' (saRNAs). Thus far, RNAa has only been demonstrated in human cells and unclear whether it conserved other mammals. In the present study, we evaluated derived from four mammalian species including nonhuman primates (African green monkey chimpanzee), mouse, rat. Previously, identified saRNAs leading to E-cadherin, p21, VEGF cells. As targeted sequences are highly...
Abstract Cancer, a prevalent and complex disease, presents significant challenge to the medical community. It is characterized by irregular cell differentiation, excessive proliferation, uncontrolled growth, invasion of nearby tissues, spread distant organs. Its progression involves interplay several elements processes. Extracellular vesicles (EVs) serve as critical intermediaries in intercellular communication, transporting molecules such lipids, RNA, membrane, cytoplasmic proteins between...
Long noncoding RNA HOX antisense intergenic myeloid 1 (HOTAIRM1) has been characterized as a critical factor in all-trans retinoic acid (ATRA)-induced differentiation of acute promyelocytic leukemia (APL) cells. However, the essential transcription for gene expression HOTAIRM1 is still unknown. Chromatin immunoprecipitation (ChIP) assays revealed that PU.1 constitutively bound to regulatory region HOTAIRM1. Co-expression led transactivation reporter assay. Detailed analysis showed two...
Sour or wild jujube fruits and dried seeds are popular food all over the world. In this study, we reported a high-quality genome assembly of sour (
Abstract Patients with primary refractory acute myeloid leukemia (AML) have a dismal long-term prognosis. Elucidating the resistance mechanisms to induction chemotherapy could help identify strategies improve AML patient outcomes. Herein, we retrospectively analyzed multiomics data of more than 1,500 cases and found that patients spliceosome mutations had higher risk developing disease. RNA splicing analysis revealed mis-spliced genes in converged on translation-associated pathways, promoted...
Abstract PU.1 (encoded by SPI1 ) is essential for myeloid development, and inhibition of its expression activity can lead to acute leukemia (AML). The precise regulation crucial the development AML, discovery circular RNAs (circRNAs) add a new layer information on regulation. Here, we found that circSPI1, RNA derived from gene, highly expressed in AML but not normal counterparts. Unlike SPI1, tumor suppressor being lowly demonstrate circSPI1 acts as an oncogene, evidenced observation...
Schistosomes are flatworm parasites that undergo a complex life cycle involving two hosts. The regulation of the parasite’s developmental processes relies on both coding RNAs and non-coding RNAs. However, roles RNAs, including long (lncRNAs) in schistosomes remain largely unexplored. Here we conduct advanced RNA sequencing male female S . japonicum during their pairing reproductive development, resulting identification nearly 8,000 lncRNAs. This extensive dataset enables us to construct...
Cotranscriptional loading of RNA processing factors onto nascent facilitates efficient gene expression. Mechanisms responsible for coupling transcription and are not well defined, but the Saccharomyces cerevisiae TREX complex provides an example. is composed subcomplex THO that associates with polymerase II required normal transcriptional elongation. proteins involved in splicing export to form larger complex. Hence, assembly physically couples elongation factors. Whether metazoan species...
The simple combinatorial rules for regulation of the sloppy-paired-1 ( slp1 ) gene by pair-rule transcription factors during early Drosophila embryogenesis offer a unique opportunity to investigate molecular mechanisms developmentally regulated repression. We find that initial repression in response Runt and Fushi-tarazu (Ftz) does not involve chromatin remodeling, or histone modification. Chromatin immunoprecipitation vivo footprinting experiments indicate RNA polymerase II (Pol II)...
Mitochondrial dysfunction is recently thought to be tightly associated with the development of cardiac hypertrophy as well hypertension. However, detailed molecular events in mitochondria at early stages hypertrophic pathogenesis are still unclear. Applying two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) combined MALDI-TOF/TOF tandem mass spectrometry, here we identified changed mitochondrial proteins left ventricular prehypertensive/hypertensive through comparing...
RNAa (RNA activation) is a mechanism by which small dsRNA (double-stranded RNA), termed saRNA (small activating target promoter sequences to induce gene expression. This technique represents novel approach overexpression without the use of exogenous DNA. In present study, we investigated whether can modulate expression development-related NANOG and manipulate cell fate. Using lentivirus-based reporter system as screening tool, identified synthetic saRNAs that stimulate in human NCCIT...
Panax Notoginseng flower saponins (PNFS) are the main active component of notoginseng (Burk) F. H. Chen bud (PNF) and possess significant anti-inflammatory efficacy. This study aims to explore mechanisms underlying PNFS' antiflammatory action in RAW264.7 macrophages.A cell counting kit-8 assay was used determine viability macrophages. Anti-inflammation effects PNFS lipopolysaccharide (LPS)-stimulated macrophages were measured based on detection nitric oxide (NO) overproduction (Griess...
<h2>Abstract</h2> The pyruvate kinase (PK) is a rate-limiting glycolytic enzyme catalyzing the dephosphorylation of phosphoenolpyruvate to pyruvate, yielding one molecule ATP. M2 isoform PK (PKM2) predominantly expressed in normal proliferating cells and tumors, both metabolic non-metabolic activities for promoting tumor cell proliferation have been identified. However, exact roles PKM2 initiation, growth maintenance are not yet fully understood. Using immunoprecipitation-coupled LC-MS/MS...
Preimplantation embryos in vivo are exposed to various growth factors the female reproductive tract that absent vitro embryo culture media. Cell-free fat extract exerts antioxidant, anti-ageing, and ovarian function-promoting effects. However, its effects on quality yet be investigated. We assessed effect of cell-free supplementation using a naturally ageing mouse model. model's efficacy influencing development pregnancy rates older women with fertilization failure. In addition, we performed...
Camellia oleifera Abel., recognized as a significant oil plant, is of immense potential health and economic value. Due to the self-incompatibility C. oleifera, pollination relies on cross-pollination from other flowers. Additionally, asynchronous flowering periods individual plants result in low fruit set yield, which limits broader cultivation utilization this species. The study investigated dynamic changes regulatory patterns different tissues within flower buds bud development flowering,...
Schistosomiasis affects over 250 million people in 78 countries. Despite praziquantel as the primary treatment, concerns about resistance schistosomes underscore need for alternative therapies. The success of RNA interference (RNAi) shows promise identifying potential drug targets to facilitate discovery. Meanwhile, double-stranded (dsRNA) is commonly used functional gene analysis via RNAi, with green fluorescent protein (ds-GFP) widely employed a control schistosome-related studies....
Metabolically robust C‐glycosides are crucial in various biological and medical applications, underscoring the need for efficient synthesis methods. While radical C‐glycosylation reactions known their reliability functional group tolerance, challenges such as glycosyl donor stability atom economy persist. In this study, we investigate underexplored potential of condition‐controlled divergent through a switchable photocatalytic strategy, involving reductive anomeric C–O bond cleavage....