A5075114655- Cardiac Arrest and Resuscitation
- Traumatic Brain Injury and Neurovascular Disturbances
- Pulmonary Hypertension Research and Treatments
- Cancer, Hypoxia, and Metabolism
- Respiratory Support and Mechanisms
- Neuroscience and Neuropharmacology Research
- Cardiac Ischemia and Reperfusion
- Extracellular vesicles in disease
- Urinary Bladder and Prostate Research
- Blood properties and coagulation
- Macrophage Migration Inhibitory Factor
- Neurotransmitter Receptor Influence on Behavior
- MicroRNA in disease regulation
- Proteoglycans and glycosaminoglycans research
- Connexins and lens biology
- Eicosanoids and Hypertension Pharmacology
- Traumatic Brain Injury Research
- Adrenal and Paraganglionic Tumors
- Emergency and Acute Care Studies
- Ultrasound in Clinical Applications
- Pituitary Gland Disorders and Treatments
- Hormonal Regulation and Hypertension
- Lipid Membrane Structure and Behavior
- Sphingolipid Metabolism and Signaling
- Neonatal and fetal brain pathology
Medical University of Vienna
2010-2024
Supplemental oxygen (O2) is used as adjunct therapy in anesthesia, emergency, and intensive care medicine. We hypothesized that excessive O2 levels (hyperoxia) can directly injure human adult cardiac myocytes (HACMs). HACMs obtained from the explanted hearts of transplantation patients were exposed to constant hyperoxia (95% O2), intermittent (alternating 10 min exposures 5% 95% normoxia (21% or mild hypoxia (5% O2) using a bioreactor. Changes cell morphology, viability assessed by lactate...
The pulmonary endothelium is an immediate recipient of high oxygen concentrations upon therapy and mediates down-stream responses. Cyclic collapse reopening atelectatic lung areas during mechanical ventilation with fractions inspired result in the propagation oscillations hypoxic/hyperoxic range. We used primary murine endothelial cell cultures to investigate responses constant oscillating conditions hypoxic hyperoxic Severe hyperoxia had pro-inflammatory cytotoxic effects including increase...
The lung can experience different oxygen concentrations, low as in hypoxia, high under supplemental therapy, or oscillating during intermittent hypoxia obstructive sleep apnea hypoxia/hyperoxia due to cyclic atelectasis the ventilated patient. This study aimed characterize oxygen-condition-specific protein composition of extracellular vesicles (EVs) released from human pulmonary microvascular endothelial cells vitro decipher their potential role biotrauma using quantitative proteomics with...
Evaluating beneficial effects of potential protective therapies following cardiac arrest in rodent models could be enhanced by exploring behavior and cognitive functions. The Morris Water Maze is a well-known paradigm to test spatial learning memory.Behavioral testing with the Sprague-Dawley rats (300 ± 25 g) resuscitated after 8 min ventricular fibrillation was carried out 5 12 weeks (CA) compared results naïve (CONTROL). At weeks, within each group latency time reach hidden platform...
Zusammenfassung Das Post-Intensive Care Syndrom (PICS) beschreibt Beeinträchtigungen der physischen, kognitiven oder psychischen Funktionen, die nach einem Intensivaufenthalt erstmals verstärkt auftreten. Diese Langzeitfolgen stellen eine Herausforderung für den Alltag Betroffener und deren Familien, aber auch das Gesundheitssystem Arbeitsmarkt dar. Die strukturierte Nachsorge durch multiprofessionelle Teams in Form von Spezialambulanzen bzw. spezifischen Rehabilitationsprogrammen verbessert...
Current resuscitation guidelines recommend that skilled persons could use ultrasound to detect reversible causes during cardiopulmonary (CPR) where the examination can be safely integrated into Advanced Life Support (ALS) algorithm. However, in a prehospital setting performing and rapidly interpreting challenging for physicians. Implementing remote, expert-guided, real-time transmissions of examinations offers opportunity tele-support, even an out-of-hospital cardiac arrest (OHCA). The aim...
Although a potential role for polyamines and transglutaminases (TGs) in memory mechanisms have been proposed, hippocampal spermine (SPM) spermidine (SPD) levels as well transamidating activity of TG spatial not addressed yet. It was therefore the aim study to assess at probe trial paradigm. C57BL/6J mice (20 animals per group) were used experiments divided into trained yoked (untrained) group. The Morris water maze (MWM) selected test, sacrificed within 5 min following hippocampi taken...
The ultraviolet (UV) absorption of various sections the human lens was studied and compared with protein expression paralleling differential UV absorbance in anterior posterior lenticular tissue. serial cryostat (60 μm) that capsules determined using a Shimadzu scanning spectrophotometer, coefficients were calculated. Two-dimensional gel electrophoresis performed two pooled extracts (anterior sections). Protein spots quantified significantly different identified by mass spectrometry...
Supplemental oxygen is frequently used together with mechanical ventilation to achieve sufficient blood oxygenation. Despite the undoubted benefits, it vigorously debated whether too much can also have unpredicted side-effects. Uncertainty due fact that molecular mechanisms are still insufficiently understood. The lung endothelium covered an exceptionally broad glycocalyx, carrying N- and O-glycans, proteoglycans, glycolipids glycosaminoglycans. Glycan structures not genetically determined...
The cornu ammonis 1 (CA1) region of the hippocampus is specifically vulnerable to global ischemia. We hypothesized that histopathological outcome in a ventricular fibrillation cardiac arrest (VFCA) rat model depends on time point examination.Male Sprague-Dawley rats were put into VFCA for 8 min, received chest compressions 2 and defibrillated achieve return spontaneous circulation. Animals surviving 80 14 days 140 compared with controls. Viable neurons counted 500 μm sector CA1 layer...
Patients presenting with insufficient tissue oxygenation and impaired lung function as in acute respiratory distress syndrome (ARDS) frequently require mechanical ventilation supplemental oxygen. Despite the being used to experiencing highest partial pressure of oxygen during healthy breathing, organ is susceptible oxygen-induced injury at supraphysiological concentrations. Hyperoxia-induced (HALI) has been regarded a second hit pre-existing ventilator-induced (VILI) attributed oxidative...
: Hemodynamic instability is common during adrenalectomy for pheochromocytoma and paraganglioma (PPGL). Most analyses focus on the risk factors intraoperative hypertension, but hypotension a frequent undesirable phenomenon PPGL surgery. This study aimed to analyze hypotensive episodes removal of PPGL, whether these are always associated with concomitant hypertensive events.
Background: Rodent models of cardiac arrest help to investigate detailed pathophysiologic mechanisms cerebral ischemia and reperfusion. Sensitive neurologic behavioral tests would enhance the ability such evaluate possible beneficial effects protective therapies. The Morris Water Maze (MWM) is a procedure study spatial learning memory. aim this was testing with MWM in rats resuscitated from ventricular fibrillation (VF) (CA) via emergency cardiopulmonary bypass (ECPB). Methods:...