Coronavirus Disease 2019 (COVID-19) is a newly emerging infectious disease caused by novel coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). After its first occurrence in Wuhan of China from December 2019, COVID-19 rapidly spread around the world. According to World Health Organization statement on 13 March 2020, there had been over 132 500 confirmed cases globally. Nevertheless, case reports children are rare, which results lack evidence for preventing and...

Early identification of plastic bronchitis (PB) is great importance and may aid in delivering appropriate treatment. This study aimed to develop validate a nomogram for predicting PB patients with refractory Mycoplasma pneumoniae pneumonia (RMPP).A total 547 consecutive children RMPP who underwent fiberoptic bronchoscopy (FOB) intervention from January 2016 June 2021 were enrolled this study. Subsequently, 374 (PB: 137, without PB: 237) December 2019 assigned the development dataset...

To analyze the clinical characteristics of Mycoplasma pneumoniae pneumonia with hypoxia in children, and identify associated risk factors MPP.A retrospective case-control study was performed on 345 children (MPP) admitted to our hospital wards from January 2017 June 2019. They were divided into three groups, namely MPP hypoxia, refractory (RMPP), general (GMPP). The features, laboratory findings, imaging, management collected compared groups.The patients (n = 69) had longer disease duration,...

Neural tube defects (NTDs) are birth of the brain, spine, or spinal cord invoked by insufficient intake folic acid in early stages pregnancy and have a complex etiology involving both genetic environmental factors. So study aimed to explore association between alterations maternal one-carbon metabolism NTDs offspring.We conducted case-control get deeper insight into this association, as well role polymorphisms. Plasma concentrations folate, homocysteine (Hcy), S-adenosylmethionine (SAM),...

Background: Acute myeloid leukemia (AML), which has a difficult prognosis, is the most common hematologic malignancy. The role of copy number variations (CNVs) and ferroptosis in tumor process becoming increasingly prominent. We aimed to identify specific CNV-driven ferroptosis-related genes (FRGs) establish prognostic model for AML. Methods: combined analysis CNV differential data differentially expressed (DEGs) from Cancer Genome Atlas (TCGA) database was performed key FRGs A risk...

Purpose: The present study clarified the effects of repetitive transcranial magnetic stimulation (rTMS) in rats with vascular dementia (VaD) and explored underlying mechanisms. Methods: Two-vessel occlusion was used as a VaD model. Two weeks afte

Acute myelogenous leukemia (AML) is a common pediatric malignancy in children younger than 15 years old. Although the overall survival (OS) has been improved recent years, mechanisms of AML remain largely unknown. Hence, purpose this study to explore differentially methylated genes and investigate underlying mechanism initiation progression based on bioinformatic analysis.Methylation array data gene expression were obtained from TARGET Data Matrix. The consensus clustering analysis was...

Succinic semialdehyde dehydrogenase (SSADH) deficiency is a rare autosomal recessively-inherited defect of γ-aminobutyric acid (GABA) metabolism. The absence SSADH, which encoded by aldehyde family 5 member A1 (ALDH5A1) gene, leads to the accumulation GABA and γ-hydroxybutyric (GHB). Few cases with SSADH were reported in China. In this study, four Chinese patients diagnosed Tianjin Children's Hospital. We conducted multidimensional analysis magnetic resonance imaging (MRI) head, semi...

Background: Acute necrotizing encephalopathy of childhood (ANE) is a rare but rapidly progressing encephalopathy. Importantly, the exact pathogenesis and evidence-based treatment scarce. Thus, we aimed to identify clinical, imaging, therapeutic characteristics that associated with prognosis pediatric ANE patients. Methods: A retrospective study was conducted on patients who were admitted Wuhan Children's Hospital between January 2014 September 2019. All cases met diagnostic criteria for...

Mycoplasma pneumoniae (MP) is a common pathogen of lower respiratory tract infection in children and adolescents. Some patients with MP are self-limiting, while the increase severe or refractory pneumonia (MPP) recent years, there great reports thromboembolism multiple organs, including lung, brain, spleen, peripheral arteries. Cardiac thrombi pulmonary embolism associated have not been reported. The most effective treatment option for cardiac thrombus was surgical resection fear detachment...

Abstract Background Cobalamin (cbl) C is a treatable rare hereditary disorder of cbl metabolism with autosomal recessive inheritance. It the most common organic acidemia, manifested as methylmalonic academia combined homocysteinemia. Early screening and diagnosis are important. The mutation spectrum MMACHC gene causing cblC varies among populations. in Chinese population notably different from that other Methods A PCR followed by high‐resolution melting curve analysis (PCR‐HRM) method...

We identified three novel mutations of the GTP cyclohydrolase 1 (GCH1) gene in patients with familial dopa-responsive dystonia (DRD), but were unable to identify meaningful sporadic no obvious family DRD background.To investigate whether GCH1 regional deletions account for etiology DRD, we screened heterozygous exonic families and DRD.Multiple ligation-dependent probe amplification analysis quantitative real-time polymerase chain reaction was performed all members our cohort controls detect...

Autoimmune encephalitis (AE) is a type of encephalopathy mediated by an antigenic immune response in the central nervous system. Most research related to autoimmune focused on early diagnosis, treatment and prognosis analysis; there has been little conducted characteristics function, relationship between function prognoses patients with needs be studied further.A total 33 children were identified through clinic database inpatient consults at Tianjin Children's Hospital from January 2013...

Myelin oligodendrocyte glycoprotein-immunoglobulin G (MOG-IgG)-associated disorders (MOGADs) have been considered as a new inflammatory disease entity of the central nervous system (CNS) and heterogeneous clinical imaging presentations. Acute disseminated encephalomyelitis (ADEM) is one most important phenotypes. Our research aimed to compare magnetic resonance (MRI) characteristics ADEM with or without MOG-IgG in pediatric-acquired demyelinating syndromes (ADSs).We retrospectively reviewed...

With the rapid increase in number of infections, children with Staphylococcus aureus (S. aureus) infection secondary to Influenza A virus (IAV), appear have a great possibility causing severe complications and illness. Despite some cases research findings regarding death IAV S. aureus, coinfection included, there were few details about successful treatment pleural empyema necrotizing pneumonia caused by methicillin-resistant (MRSA) following IAV. In this case report, we describe clinical...

Abstract Background To screen the single nucleotide polymorphisms (SNPs) in coding regions of VANGL and FZD family members related to plane cell polarity (PCP) signaling pathway neural tube defects (NTDs) patients, so as provide theoretical experimental basis for prevention treatment NTDs by intervening PCP signal transduction. Methods 112 patients were collected case group craniocerebral trauma control. Afterwards, blood genomic DNA was extracted sequenced. The distribution SNP alleles...

<title>Abstract</title> <bold>Background</bold>Spinal muscular atrophy (SMA) is characterized by motor neuron degeneration and progressive muscle weakness. Clinical trials real-world studies have demonstrated significant clinically meaningful benefits of Nusinersen in a broad range symptomatic presymptomatic patients. The parameters serum to monitor the safety effect was rarely reported among pediatrics This retrospective study aimed characterize children with SMA during...

Cow's milk allergy (CMA) is the most common IgE-mediated food and Bos d 5 major allergen in cow's proteins. More than 60% of patients with CMA are sensitized to this protein.A recombinant protein, encoded by a synthetic gene consisting reassembled fragments, was expressed E. coli strain BL21 (DE3) cells purified homogeneity. The B5M lacked relevant IgE-reactivity allergenic activity compared dot-blot basophil activation assays. T-cell proliferation experiments demonstrated that preserved...