A5062405573- Blood Coagulation and Thrombosis Mechanisms
- Fermentation and Sensory Analysis
- Probiotics and Fermented Foods
- Platelet Disorders and Treatments
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Vitamin K Research Studies
- Venous Thromboembolism Diagnosis and Management
- Hemophilia Treatment and Research
- Blood properties and coagulation
- Polyamine Metabolism and Applications
- Antiplatelet Therapy and Cardiovascular Diseases
- Protease and Inhibitor Mechanisms
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Atrial Fibrillation Management and Outcomes
- Metal-Catalyzed Oxygenation Mechanisms
- Hops Chemistry and Applications
- Genomics and Phylogenetic Studies
- Identification and Quantification in Food
- Connexins and lens biology
- Horticultural and Viticultural Research
- Venomous Animal Envenomation and Studies
- Food Quality and Safety Studies
- Complement system in diseases
- Pulmonary Hypertension Research and Treatments
- Glycosylation and Glycoproteins Research
Yokohama City University
2025
Yokohama City University Medical Center
2025
Asahi Breweries (Japan)
2009-2024
University of Fukui
2024
National Hospital Organization
2024
Suzuka University of Medical Science
2014-2023
Hamamatsu University School of Medicine
2019-2021
National Research Institute of Brewing
2018-2021
University of Shizuoka
2019-2020
Osaka Ohtani University
2020
Thrombin-activated vitamin K-dependent protein C purified from human plasma has a potent anticoagulant effect on plasma, whereas its bovine counterpart very weak plasma. This species difference was found to be partly due more rapid degradation of factor Va by than activated C. In the presence phospholipid, cleaves several peptide bonds in fragment D (heavy chain Va), F1F2 (light Va) there appears only one bond that is slowly cleaved. The accompanied parallel loss V activity. With blood...
Components of the electron transport system involved in steroid Ilo-hydroxylation pig adrenal cortex are a flavoprotein (adrenodoxin reductase), non-heme iron protein (adrenodozin), and possibly cytochrome Paw.Reconstitution reduced nicotinamide adenine dinucleotide phosphate Pdu reductase was achieved from soluble components.Adrenodozin contains 2 atoms moles labile suliide per mole, has molecular weight approximately 20,000, high oxidation-reduction potential.The atoms, which ferric...
Protein C inhibitor was isolated from human plasma using conventional chromatographic technique consisting of barium citrate adsorption, polyethylene glycol fractionation, DEAE-Sepharose CL-GB treatment, ammonium sulfate dextran sulfate-agarose chromatography, gel filtration on ACA-44, and DEAE-Sephacel chromatography.The purified protein is a single polypeptide chain with an apparent M, = 57,000 sodium dodecyl sulfate-polyacrylamide electrophoresis.The heterogeneous in PI: six PIS exist...
A cDNA library in X-phage Xgtll containing DNA inserts prepared from human liver mRNA was screened with monoclonal antibodies to protein C inhibitor.Six positive clones were isolated 6 X IO6 phages and plaque purified.The the phage largest insert, which hybridized a probe on basis of amino-terminal amino acid sequence mature inhibitor, sequenced.This insert contained 2106 base pairs coding for 5'-noncoding region, 19-amino signal peptide, 387-amino protein, stop codon, long 3"noncoding...
Beer has been generally recognized as a microbiologically stable beverage. However, microbiological incidents occasionally occur in the brewing industry. The instability of beer is often caused by bacteria consisting four genera, Lactobacillus, Pediococcus, Pectinatus and Megasphaera. Lactobacillus Pediococcus belong to lactic acid (LAB), whereas Megasphaera form group strict anaerobes that are known intermediates between Gram-positive Gram-negative bacteria. frequencies spoilage these...
Abstract Chediak‐Higashi syndrome (CHS) is a rare autosomal recessive disorder characterized by severe immunologic defects, reduced pigmentation, bleeding tendency, and progressive neurological dysfunction. Most patients present in early childhood die unless treated bone marrow transplantation. About 10–15% of exhibit much milder clinical phenotype survive to adulthood, but develop often fatal Very an intermediate adolescent CHS phenotype, presenting with infections childhood, course...
It is well recognized that activation of the coagulation system plays an important role in bleomycin (BLM)-induced lung injury and fibrosis. The protein C (PC) pathway regulator system. In this study, we evaluated bronchoalveolar lavage fluid (BALF) concentration activated PC (APC) therapeutic effect intratracheal administration APC on BLM-induced fibrosis mice. levels BALF were significantly lower BLM-treated animals than saline-treated group. Fibrotic changes progressive mice treated with...
Journal Article Mechanism of Inhibition Activated Protein C by Inhibitor Get access Koji SUZUKI, SUZUKI 2 Department Laboratory Medicine, Mie University School MedicineTsu-city, 514 To whom correspondence should be addressed. Search for other works this author on: Oxford Academic PubMed Google Scholar Junji NISHIOKA, NISHIOKA Hiroshi KUSUMOTO, KUSUMOTO Senichiro HASHIMOTO The Biochemistry, Volume 95, Issue 1, 1984, Pages 187–195, https://doi.org/10.1093/oxfordjournals.jbchem.a134583...
Bovine plasma CIg, like human is a glycoprotein with molecular weight of approximately 450,000 daltons and consists two homologous subunits, the alpha beta chains. These subunits are covalently linked through disulfide bridges in their carboxyl terminal domains. The regions presumed to contain fibrin-reactive transamidation site. covalent incorporation CIg into fibrin has been conclusively demonstrated by isolation S-carboxymethyl derivative CIg-fibrin-alpha chain complex determination its...
Abstract Suckling elevated serum prolactin but had no effect on circulating thyrotropin, tri-iodothyronine and thyroxine in seven post-partum women. Injections of 6 300 μg thyrotropin-releasing hormone raised concentrations both not thyroxine. One or 2 either although three subjects, caused similar relative increases thyrotropin. Lactotrophs thyrotrophs the pituitary gland appear to be similarly responsive exogenous hormone, failure observe thyrotropin release with suckling is due blockade...
Deregulated interplay between inflammation and coagulation plays a pivotal role in the pathogenesis of sepsis. Therapeutic approaches that simultaneously target both hold great promise for treatment Thrombomodulin is an endogenous anticoagulant protein that, cooperation with C thrombin-activatable fibrinolysis inhibitor, serves to maintain endothelial microenvironment anti-inflammatory state. A recombinant soluble form thrombomodulin has been approved treat patients suffering from...
Plasma thrombomodulin (TM) levels were significantly elevated at disease onset in patients with thrombotic thrombocytopenic purpura (TTP) and disseminated intravascular coagulation (DIC), but was not those essential thrombocythemia idiopathic purpura. However, TTP DIC, TM decreased after they achieved complete remission. In both DIC patients, plasma poor prognosis higher than that good prognosis. Among the level organ failure without, there no differences among various underlying diseases...
We found functionally active thrombomodulin in human platelets (60±18 molecules per platelet). Protein C appeared not to be activated by thrombin with gel-filtered platelets. However, the activation of protein was accelerated thrombin-stimulated and washed This cofactor activity neutralized anti-lung thrombomodulin-F(ab′)2 From Triton X-extract platelets, partially purified diisopropylphosphoryl-thrombin-agarose affinity chromatography. The Mr predominant platelet 78,000 before 109,000 after...
Hypofibrinolysis is a common finding in patients with diabetes mellitus (DM) and obesity risk factor for the development of cardiovascular disease. Recently, new potent inhibitor fibrinolysis, thrombin-activatable fibrinolysis (TAFI) has been isolated characterized from human plasma. The present study was undertaken to assess activity circulating level TAFI its relation fibrinolytic function type 2 DM. Fifty-seven DM (38 men, 19 women) were enrolled this study. categorized age-matched obese...
The three-dimensional structure of the iron-containing superoxide dismutase (EC 1.15.1.1) from Pseudomonas ovalis has been determined at 2.9-A resolution by method multiple isomorphous replacement. molecule is a dimer two identical subunits with iron atom per monomer. conformation enzyme completely different that eukaryotic copper-zinc dismutase. Each subunit consists about 50% alpha-helix plus three strands antiparallel pleated sheet. atoms are coordinated four protein ligands, one which...
Abstract Endothelial monolayers have shown the ability to signal each other through gap junctions. Gap junction-mediated cell-cell interactions been implicated in modulation of endothelial cell functions during vascular inflammation. Inflammatory mediators alter mechanical properties cells, although exact role junctions this process remains unclear. Here, we sought study regulation stiffness, an important physical feature that is associated with many pathologies. The cellular stiffness...
The preventive efficacies and safety of Emblica Officinalis Gatertn (Amla), a most important extensively studied plant in the traditional Indian Ayurvedic system medicine, are presented. Eligible healthy adult subjects (n = 15) were randomized to receive either amla or placebo (500 mg per day) during an 18-week study. efficacy parameters evaluated vascular function, blood hematology, oxidative inflammatory biomarkers, glucose lipid profiles, urinalysis, liver hepatotoxicity. intake showed...