A5100403423- Plant Molecular Biology Research
- Plant Gene Expression Analysis
- Postharvest Quality and Shelf Life Management
- Plant Physiology and Cultivation Studies
- Plant Stress Responses and Tolerance
- Photosynthetic Processes and Mechanisms
- Plant responses to water stress
- Light effects on plants
- Plant Reproductive Biology
- Plant Virus Research Studies
- CRISPR and Genetic Engineering
- Plant and Fungal Interactions Research
- Antibiotic Resistance in Bacteria
- Plant biochemistry and biosynthesis
- Photoreceptor and optogenetics research
- RNA Interference and Gene Delivery
- Legume Nitrogen Fixing Symbiosis
- Cassava research and cyanide
- Plant Pathogens and Fungal Diseases
- Salmonella and Campylobacter epidemiology
- Plant and animal studies
- RNA and protein synthesis mechanisms
- Chromosomal and Genetic Variations
- Phytochemical Studies and Bioactivities
- Entomological Studies and Ecology
Huazhong Agricultural University
2009-2025
Institute of Grassland Research
2025
Sichuan Agricultural University
2024
Ministry of Agriculture and Rural Affairs
2022-2024
Shanghai Zhangjiang Laboratory
2021-2024
Yunnan Academy of Agricultural Sciences
2024
Inner Mongolia University
2024
Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences
2024
Inner Mongolia Agricultural University
2024
Yanbian University
2023
The position-dependent specification of the hair and non-hair cell types in Arabidopsis root epidermis provides a simple model for study fate determination plants. Several putative transcriptional regulators are known to influence this decision. Indirect evidence from studies with maize R gene has been used suggest that bHLH transcription factor also participates process. We show two genes encoding proteins, GLABRA3(GL3) ENHANCER OF GLABRA3 (EGL3), act partially redundant manner specify...
Lilies are economically important monocots known for their ornamental flowers, bulbs, and large genomes. The absence of genomic information has impeded evolutionary studies genome-based breeding efforts. Here, we present reference genomes Lilium sargentiae (lily, 35.66 Gb) Gloriosa superba (flame lily, 5.09 Gb). giant lily genome is shaped by recent long terminal repeat retroelements. Phylogenetic analysis reveals diverse, independent origins cultivars. Gene families involved in sucrose...
Significance Upon receipt of different cues, transcription factors bind to specific DNA sequences recruit the general transcriptional machinery for gene expression. Chromatin modification plays a central role in regulation expression by providing and with dynamic access an otherwise tightly packaged genome. We use Arabidopsis study how chromatin perceives ethylene signaling, important plant hormone growth, development, stress responses. demonstrate that essential factor EIN2, which mediates...
Abstract Ethylene gas is essential for many developmental processes and stress responses in plants. EIN2 plays a key role ethylene signalling but its function remains enigmatic. Here, we show that specifically elevates acetylation of histone H3K14 the non-canonical H3K23 etiolated seedlings. The up-regulation these two marks positively correlates with ethylene-regulated transcription activation, elevation requires EIN2. Both EIN3 interact SANT domain protein named nuclear associated 1...
Ethylene plays pleiotropic roles in plant growth, development, and stress responses. Although the effects of ethylene on plants are well documented, little is known about molecular-level events that result transcriptional repression during response. In this study, we found two histone deacetylases, SRT1 SRT2, interact with ENAP1, which associates EIN2 nucleus. Genetic transcriptome analyses revealed SRT2 required for negative regulation certain ethylene-responsive genes. The acetylation...
Summary Dynamic DNA methylation regulatory networks are involved in many biological processes. However, how patterns change during flower senescence and their relevance with gene expression related molecular mechanism remain largely unknown. Here, we used whole genome bisulfite sequencing to reveal a significant increase of the promoter region genes natural ethylene‐induced carnation ( Dianthus caryophyllus L.), which was correlated decreased demethylase DcROS1 . Silencing accelerated while...
The (6-4) photolyases use blue light to reverse UV-induced photoproducts in DNA. This photorepair was thought be restricted eukaryotes. Here we report a prokaryotic photolyase, PhrB from Agrobacterium tumefaciens , and propose that are broadly distributed prokaryotes. crystal structure of photolyase related protein B (PhrB) at 1.45 Å resolution suggests DNA binding mode different the eukaryotic counterparts. A His-His-X-X-Arg motif is located within proposed lesion contact site PhrB....
Carnation (Dianthus caryophyllus L.) is one of the most important and typical ethylene sensitive cut flowers worldwide, although how influences petal senescence process in carnation remains largely unknown. Here, we screened out key transcription factors, DcWRKY75, using a constructed induced transcriptome found that it shows quick induction by treatment. Silencing DcWRKY75 delays carnation. Molecular evidence confirms can bind to promoter regions two main biosynthetic genes (DcACS1 DcACO1)...
Abstract Petal senescence is controlled by a complex regulatory network. Epigenetic regulation like histone modification influences chromatin state and gene expression. However, the involvement of methylation in regulating petal remains poorly understood. Here, we found that trimethylation H3 at Lysine 4 (H3K4me3) increased during ethylene-induced carnation (Dianthus caryophyllus L.). H3K4me3 levels were positively associated with expression transcription factor DcWRKY75, ethylene...
Histone acetylation and deacetylation are essential for gene regulation have been implicated in the of plant hormone responses. Many studies indicated role histone ethylene signaling; however, few investigated how signaling regulates genomic landscape chromatin states. Recently, we found that can specifically elevate H3K14 non-canonical H3K23 etiolated seedlings activation is positively associated with elevation H3K14Ac H3K23Ac response to ethylene. To assess H3K9, H3K14, modifications...
Abstract Ethylene is an important phytohormone with pleotropic roles in plant growth, development, and stress responses. ETHYLENE INSENSITIVE2 (EIN2) mediates the transduction of ethylene signal from endoplasmic reticulum membrane to nucleus, where its C-terminus (EIN2-C) regulates histone acetylation mediate transcriptional regulation by EIN3. However, no direct interaction between EIN2-C EIN3 has been detected. To determine how act together, we followed a synthetic approach engineered...
SUMMARY Carnation ( Dianthus caryophyllus L.) is one of the most famous and ethylene‐sensitive cut flowers worldwide, but how ethylene interacts with other plant hormones factors to regulate petal senescence in carnation largely unknown. Here we found that a gene encoding WRKY family transcription factor, DcWRKY33 , was significantly upregulated upon treatment. Silencing overexpression could delay accelerate petals, respectively. Abscisic acid (ABA) H 2 O treatments also petals by inducing...
Histone acetylation has been shown to involve in stress responses. However, the detailed molecular mechanisms that how histone deacetylases and transcription factors function drought response remain be understood. In this research, we show ENAP1 ENAP2 are positive regulators of tolerance plants, enap1enap2 double mutant is more sensitive stress. Both interact with MYB44, a factor interacts deacetylase HDT4. Genetics data myb44 null mutation enhances sensitivity Whereas, HDT4 negatively...
Clonostachys, a genus with rich morphological and ecological diversity in Bionectriaceae, has wide distribution among diverse habitats. Several studies have reported Clonostachys fungi as effective biological agents against multiple fungal plant pathogens. To clarify the biocontrol mechanisms of fungi, this study was undertaken to sequence assemble genomes two C. chloroleuca one rhizophaga. Here, we performed genomic sequencing three strains collected from China General Microbiological...
Abstract The C2H2 zinc finger proteins (ZFPs) play essential roles in regulating cold stress responses. Similarly, raffinose accumulation contributes to freezing tolerance. However, the relationship between functions and synthesis tolerance remains uncertain. Here, we report characterization of cold-induced C2H2-type protein PhZFP1 Petunia hybrida. was found be predominantly localized nucleus. Overexpression conferred enhanced transgenic petunia lines. In contrast, RNAi mediated suppression...
Although numerous transcription factors with antagonistic activities have been shown to contribute growth and development, whether how they regulate senescence in plants is largely unknown. In this study, we investigated the role of petal carnation (Dianthus caryophyllus), one most common types ethylene-sensitive cut flowers produced worldwide. We identified DcHB30 that encodes a ZF-HD factor down-regulated ethylene-treated transcriptomes. found silencing accelerated ethylene-induced...
Carnation (Dianthus caryophyllus L.) is a respiratory climacteric flower, comprising one of the most important cut flowers that extremely sensitive to plant hormone ethylene. Ethylene signaling core transcription factor DcEIL3-1 plays key role in ethylene induced petal senescence carnation. However, how dose regulated carnation process still not clear. Here, we screened out two EBF (EIN3 Binding F-box) genes, DcEBF1 and DcEBF2, which showed quick elevation by treatment according...
Photolyases can repair pyrimidine dimers on the DNA that are formed during UV irradiation. PhrB from Agrobacterium fabrum represents a new group of prokaryotic (6–4) photolyases which contain an iron-sulfur cluster and DMRL chromophore. We performed site-directed mutagenesis in order to assess role particular amino acid residues photorepair photoreduction, FAD chromophore converts oxidized enzymatically active, reduced form. Our study showed Trp342 Trp390 serve as electron transmitters. In...
Ethylene is one of the most important hormones for plant developmental processes and stress responses. However, phosphorylation regulation in ethylene signaling pathway largely unknown. Here we report cap binding protein 20 (CBP20) at Ser245 regulated by ethylene, involved root growth. The constitutive mimic form CBP20 (CBP20S245E or CBP20S245D), while not de-phosphorylation (CBP20S245A) able to rescue responsive phenotype cbp20. By genome wide study with gene expression microRNA (miRNA)...
Smart farming uses advanced tools and technologies such as intelligent agricultural machines, high-precision sensors, navigation systems, sophisticated computer systems to increase the economic benefits of agriculture reduce associated human effort. With increasing demands individualized operations, internet things is a crucial technique for acquiring, monitoring, processing, managing resource data precision ecological monitoring domains. Here, an things-based system scheme integrating most...
Abstract Polyploidization leads to novel phenotypes and is a major force in evolution. However, the relationship between evolution of new traits variations post-translational modifications (PTM) proteins during polyploidization has not been studied. Acetylation lysine residues common protein PTM that plays critical regulatory role central metabolism. To test whether changes metabolism citrus fruit associated with reprogramming acetylation (Kac) non-histone allotetraploidization, we performed...