A5043553623- Inhalation and Respiratory Drug Delivery
- Bacteriophages and microbial interactions
- Microbial infections and disease research
- Pneumonia and Respiratory Infections
- Respiratory viral infections research
- Monoclonal and Polyclonal Antibodies Research
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Protease and Inhibitor Mechanisms
- Fatty Acid Research and Health
- Cell Adhesion Molecules Research
- Herbal Medicine Research Studies
- Blood Coagulation and Thrombosis Mechanisms
- Asthma and respiratory diseases
- COVID-19 and healthcare impacts
- Immune Response and Inflammation
- Peptidase Inhibition and Analysis
- Neonatal Respiratory Health Research
- Advanced Drug Delivery Systems
- Metabolomics and Mass Spectrometry Studies
- Lipid metabolism and biosynthesis
- Toxin Mechanisms and Immunotoxins
- Infection Control and Ventilation
- Protein purification and stability
- Thermal Regulation in Medicine
- Air Quality Monitoring and Forecasting
Université de Tours
2018-2024
Inserm
2015-2024
Clinical Investigation Center Plurithematic Tours
2024
Centre d'Étude des Pathologies Respiratoires
2018-2023
Bacteriophages have been identified as a potential treatment option to treat lung infection in the context of antibiotic resistance. We performed preclinical study predict efficacy delivery bacteriophages against Pseudomonas aeruginosa (PA) when administered via nebulization during mechanical ventilation (MV). selected mix four anti-PA phages containing two Podoviridae and Myoviridae, with coverage 87.8% (36/41) on an international PA reference panel. When nebulization, loss 0.30–0.65 log...
BACKGROUND AND PURPOSE 255: Pseudomonas aeruginosa is a main cause of ventilator-associated pneumonia (VAP) with drug-resistant bacteria. Bacteriophage therapy has experienced resurgence to compensate for the limited development novel antibiotics. However, phage compassionate use so far, resulting from lack adequate studies in relevant pharmacological models. We used pig model caused by P. that recapitulates essential features human disease study antimicrobial efficacy nebulized-phage...
Therapeutic antibodies (Abs) are emerging as major drugs to treat respiratory diseases, and inhalation may provide substantial benefits for their delivery. Understanding the behavior of Abs after pulmonary deposition is critical development. We investigated pharmacokinetics a nebulized Ab by continuous sampling in lung parenchyma using microdialysis non-human primates. defined optimal conditions demonstrated that feasible over period several days. The concentration-profile indicated...
Abstract Kallikrein-related peptidase 12 (KLK12) is a kallikrein family involved in angiogenesis – complex biological process which the sprouting, migration and stabilization of endothelial cells requires extracellular matrix remodeling. To characterize molecular mechanisms associated with KLK12′s proangiogenic activity, we evaluated its ability to hydrolyze various proteins. Our results show that KLK12 efficiently cleaved human proteins fibronectin tenascin, both are regulation cell...
Background: The coronavirus infectious disease-2019 (COVID-19) pandemic has led to an unprecedented shortage of healthcare resources, primarily personal protective equipment like surgical masks, and N95/filtering face piece type 2 (FFP2) respirators. Objective: Reuse masks N95/FFP2 respirators may circumvent the supply chain constraints thus overcome mass shortage. Methods, design, setting, measurement: Herein, we tested effects dry- moist-air controlled heating treatment on structure...
Non-Human Primates (NHPs) are particularly relevant for preclinical studies during the development of inhaled biologics. However, aerosol inhalation in NHPs is difficult to evaluate due a low lung deposition fraction and high variability. The objective this study was influence mesh nebulizer parameters improve macaques. We developed humidified heated ventilated anatomical 3D printed macaque model upper respiratory tract reduce experiments with animals. compared vivo using 2D planar...
Ether lipids are composed of alkyl with an ether bond at the sn-1 position a glycerol backbone and alkenyl lipids, which possess vinyl glycerol. These glycerolipids present either as polar glycerophospholipids or neutral glycerolipids. Before studying biological role molecular species glycerolipids, there is need to separate quantify total from samples in order determine any variation depending on tissue physiopathological conditions. Here, we detail development first high-performance...
Abstract Novel therapeutic strategies are urgently needed to combat pneumonia caused by Streptococcus pneumoniae strains resistant standard-of-care antibiotics. Previous studies have shown that targeted stimulation of lung innate immune defenses through intranasal administration the Toll-like receptor 5 agonist flagellin, improves treatment when combined with To promote translation clinic application, this study assessed direct delivery flagellin airways nebulization using a vibrating mesh...
Novel therapeutic strategies are urgently needed to combat pneumonia caused by
<i>Pseudomonas aeruginosa</i> (<i>Psa</i>) lung infections are increasingly difficult to treat due evolving antibiotic resistance; bacteriophages (phages) part of the foreseen complementary/alternative therapies. In this study, we developed a phage cocktail for airways delivery and assessed its preclinical efficacy resistance aerosolization. A was tested against 43-strain <i>Psa</i> reference panel published in 2013. Its <i>in vivo</i> murine model acute infection, obtained by pulmonary...
Background and Purpose. Pseudomonas aeruginosa is a main cause of ventilator-associated pneumonia (VAP) with drug-resistant bacteria. Bacteriophage therapy has experienced resurgence to compensate for the limited development novel antibiotics. However, phage compassionate use so far, resulting from lack adequate studies in relevant pharmacological models. We used pig model VAP caused by P. that recapitulates essential features human disease study antimicrobial efficacy nebulized-phage...