Nidhal Kahlaoui
A5010608795- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- SARS-CoV-2 detection and testing
- COVID-19 epidemiological studies
- Immunotherapy and Immune Responses
- Tuberculosis Research and Epidemiology
- Animal Virus Infections Studies
- Immune Response and Inflammation
- Infectious Encephalopathies and Encephalitis
- Virus-based gene therapy research
- CAR-T cell therapy research
- Influenza Virus Research Studies
- Cell Image Analysis Techniques
- Dermatology and Skin Diseases
- COVID-19 diagnosis using AI
- Asthma and respiratory diseases
- Microfluidic and Capillary Electrophoresis Applications
- Respiratory viral infections research
- Viral gastroenteritis research and epidemiology
- Inhalation and Respiratory Drug Delivery
- Mosquito-borne diseases and control
- Vaccine Coverage and Hesitancy
- Air Quality Monitoring and Forecasting
- Viral Infections and Outbreaks Research
- Genomics and Phylogenetic Studies
Infectious Disease Models and Innovative Therapies
2020-2024
Inserm
2020-2024
Université Paris-Saclay
2020-2024
CEA Paris-Saclay
2020-2024
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2020-2024
CEA Paris-Saclay - Etablissement de Fontenay-aux-roses
2020-2023
Centre International de Recherche en Infectiologie
2020
Virologie et Pathologies Humaines
2020
Université Claude Bernard Lyon 1
2020
Aix-Marseille Université
2020
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic is continuing to disrupt personal lives, global healthcare systems, and economies. Hence, there an urgent need for a vaccine that prevents viral infection, transmission, disease. Here, we present two-component protein-based nanoparticle displays multiple copies of the SARS-CoV-2 spike protein. Immunization studies show this induces potent neutralizing antibody responses in mice, rabbits, cynomolgus macaques....
The SARS-CoV-2 pandemic has affected more than 185 million people worldwide resulting in over 4 deaths. To contain the pandemic, there is a continued need for safe vaccines that provide durable protection at low and scalable doses can be deployed easily. Here, AAVCOVID-1, an adeno-associated viral (AAV), spike-gene-based vaccine candidate demonstrates potent immunogenicity mouse non-human primates following single injection confers complete from challenge macaques. Peak neutralizing antibody...
Non-human primates infected with SARS-CoV-2 exhibit mild clinical signs. Here we used a mathematical model to characterize in detail the viral dynamics 31 cynomolgus macaques for which nasopharyngeal and tracheal load were frequently assessed. We identified that cells had large burst size (>10 4 virus) within-host reproductive basic number of approximately 6 compartment, respectively. After peak load, rapidly lost half-life 9 hours, no significant association between cytokine elevation...
Abstract Effective treatments against Severe Acute Respiratory Syndrome coronavirus 2 (SARS-CoV-2) are urgently needed. Monoclonal antibodies have shown promising results in patients. Here, we evaluate the vivo prophylactic and therapeutic effect of COVA1-18, a neutralizing antibody highly potent B.1.1.7 isolate. In both settings, SARS-CoV-2 remains undetectable lungs treated hACE2 mice. Therapeutic treatment also causes reduction viral loads Syrian hamsters. When administered at 10 mg kg-1...
Abstract The COVID-19 pandemic has exemplified that rigorous evaluation in large animal models is key for translation from promising vitro results to successful clinical implementation. Among the drugs have been largely tested trials but failed so far bring clear evidence of efficacy favipiravir, a nucleoside analogue with spectrum activity against several RNA viruses and small models. Here, we evaluate antiviral favipiravir Zika or SARS-CoV-2 virus cynomolgus macaques. In both models, high...
Abstract COVID-19 has become a pandemic that caused over 200,000 deaths worldwide, with no antiviral drug or vaccine yet available. Several clinical studies are ongoing to evaluate the efficacy of repurposed drugs have demonstrated in vitro . Among these candidates, hydroxychloroquine (HCQ) been given thousands individuals worldwide but definitive evidence for HCQ treatment is still missing.We evaluated activity both and SARS-CoV-2-infected macaques. showed monkey African green kidney...
Abstract Achieving sufficient worldwide vaccination coverage against SARS-CoV-2 will require additional approaches to currently approved viral vector and mRNA vaccines. Subunit vaccines may have distinct advantages when immunizing vulnerable individuals, children pregnant women. Here, we present a new generation of subunit targeting antigens CD40-expressing antigen-presenting cells. We demonstrate that the receptor-binding domain (RBD) spike protein CD40 (αCD40.RBD) induces significant...
The definition of correlates protection is critical for the development next-generation SARS-CoV-2 vaccine platforms. Here, we propose a model-based approach identifying mechanistic based on mathematical modelling viral dynamics and data mining immunological markers. application to three different studies in non-human primates evaluating vaccines CD40-targeting, two-component spike nanoparticle mRNA 1273 identifies quantifies two main mechanisms that are decrease rate cell infection an...
Abstract After clean drinking water, vaccination is the most impactful global health intervention. However, development of new vaccines against difficult-to-target diseases hampered by lack diverse adjuvants for human use. Of particular interest, none currently available induce Th17 cells. Here, we develop and test an improved liposomal adjuvant, termed CAF®10b, that incorporates a TLR-9 agonist. In head-to-head study in non-human primates (NHPs), immunization with antigen adjuvanted CAF®10b...
Abstract One year into the Coronavirus Disease 2019 (COVID-19) pandemic caused by Severe Acute Respiratory Syndrome coronavirus 2 (SARS-CoV-2), effective treatments are still needed 1–3 . Monoclonal antibodies, given alone or as part of a therapeutic cocktail, have shown promising results in patients, raising hope that they could play an important role preventing clinical deterioration severely ill exposed, high risk individuals 4–6 Here, we evaluated prophylactic and effect COVA1-18 vivo ,...
The COVID-19 pandemic continues to have devastating consequences on health and economy, even after the approval of safe effective vaccines. Waning immunity, emergence variants concern, breakthrough infections, lack global vaccine access acceptance perpetuate epidemic. Here, we demonstrate that a single injection an adenoassociated virus (AAV)-based elicits at least 17-month-long neutralizing antibody responses in non-human primates levels were previously shown protect from viral challenge....
Non-human primates (NHPs) are particularly relevant as preclinical models for SARS-CoV-2 infection and nuclear imaging may represent a valuable tool monitoring in this species. We investigated the benefit of computed X-ray tomography (CT) [18F]-FDG positron emission (PET) to monitor early phase disease large cohort (n = 76) infected macaques. Following infection, animals showed mild COVID-19 symptoms including typical lung lesions. CT scores at acute reflect heterogeneity burden following...
Development of new vaccines tailored for difficult-to-target diseases is hampered by a lack diverse adjuvants human use, and none the currently available induce Th17 cells. Here, we develop liposomal adjuvant, CAF®10b, that incorporates Mincle Toll-like receptor 9 agonists. In parallel mouse non-human primate studies comparing to CAF® already in clinical trials, report species-specific effects adjuvant composition on quality magnitude responses. When combined with antigen, CAF®10b induces...
Abstract Non-human primates infected with SARS-CoV-2 exhibit mild clinical signs. Here we used a mathematical model to characterize in detail the viral dynamics 31 cynomolgus macaques 10 6 pfu of for which nasopharyngeal and tracheal load were frequently assessed. We identified that cells had large daily production (>10 4 virus) within-host reproductive basic number compartment, respectively. After peak load, rapidly cleared half-life 9 hours, no significant association between cytokine...
Summary The SARS-CoV-2 pandemic is continuing to disrupt personal lives, global healthcare systems and economies. Hence, there an urgent need for a vaccine that prevents viral infection, transmission disease. Here, we present two-component protein-based nanoparticle displays multiple copies of the spike protein. Immunization studies show this induces potent neutralizing antibody responses in mice, rabbits cynomolgus macaques. vaccine-induced immunity protected macaques against high dose...
The SARS-CoV-2 pandemic is continuing to disrupt personal lives, global healthcare systems and economies. Hence, there an urgent need for a vaccine that prevents viral infection, transmission disease. Here, we present two-component protein-based nanoparticle displays multiple copies of the spike protein. Immunization studies show this induces potent neutralizing antibody responses in mice, rabbits cynomolgus macaques. vaccine-induced immunity protected macaques against high dose challenge,...
Abstract Introduction A Vereos PET/CT device was adapted to be compatible with the experimentation in large animals within BSL-3 environment. The aim of this study investigate impact modification on performance according NEMA NU2-2012 standard. Methods Spatial resolution, sensitivity, count rate performance, accuracies corrections and image quality were assessed using standards before after installation a transparent poly-methyl methacrylate tube 8 mm thickness, 680 diameter 2800 long inside...
Abstract Controlling the circulation of recently emerged SARS-CoV-2 in human populations requires massive vaccination campaigns. Achieving sufficient worldwide coverage will require additional approaches to first generation approved viral vector and mRNA vaccines. Subunit vaccines have excellent safety efficacy records may distinct advantages, particular when immunizing individuals with vulnerabilities or considering children pregnant women. We developed a new subunit enhanced immunogenicity...
Abstract The definition of correlates protection is critical for the development next generation SARS-CoV-2 vaccine platforms. Here, we propose a new framework identifying mechanistic based on mathematical modelling viral dynamics and data mining immunological markers. application to three different studies in non-human primates evaluating vaccines CD40-targeting, two-component spike nanoparticle mRNA 1273 identifies quantifies two main mechanisms that are decrease rate cell infection an...