A5041416638- Hepatitis C virus research
- interferon and immune responses
- NF-κB Signaling Pathways
- Liver Disease Diagnosis and Treatment
- Cell death mechanisms and regulation
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Hepatitis B Virus Studies
- Microbial Natural Products and Biosynthesis
- Monoclonal and Polyclonal Antibodies Research
- Marine Sponges and Natural Products
- Axial and Atropisomeric Chirality Synthesis
- Traditional and Medicinal Uses of Annonaceae
- Hematopoietic Stem Cell Transplantation
- Cancer-related Molecular Pathways
- Molecular spectroscopy and chirality
- Synthesis and Biological Activity
- Quinazolinone synthesis and applications
- Immune Response and Inflammation
- Angiogenesis and VEGF in Cancer
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Antimicrobial Resistance in Staphylococcus
- Ubiquitin and proteasome pathways
- Cytokine Signaling Pathways and Interactions
- Glycosylation and Glycoproteins Research
National Research Centre
2015-2024
Universitätsklinikum Würzburg
2018-2024
Czech Academy of Sciences, Institute of Biotechnology
2022
National Water Research Center
2010
In the present study, LC-HRESIMS-assisted dereplication along with bioactivity-guided isolation led to targeting two brominated oxindole alkaloids (compounds 1 and 2) which probably play a key role in previously reported antibacterial, antibiofilm, cytotoxicity of Callyspongia siphonella crude extracts. Both metabolites showed potent antibacterial activity against Gram-positive bacteria, Staphylococcus aureus (minimum inhibitory concentration (MIC) = 8 4 µg/mL) Bacillus subtilis (MIC 16...
Staphylococcus aureus is the leading cause of skin and soft tissue infections a major health burden due to emergence antibiotic-resistant strains. To address unmet need alternative treatments antibiotics, better understanding protective immune mechanisms against S. infection warranted. Here, we report that tumor necrosis factor (TNF) promoted protection in skin, which was mediated by bone marrow–derived cells. Furthermore, neutrophil-intrinsic TNF receptor (TNFR) signaling directed immunity...
Three new naphthylisoquinoline dimers, jozibrevines A–C (1a–c), were isolated from the West African shrub Ancistrocladus abbreviatus, along with known dimer jozimine A2 (1d). The two molecular moieties of 1a–d are coupled via sterically constrained 3′,3″-positions their naphthalene units, so that central biaryl linkage is rotationally hindered. With outer axes also being chiral, possess three consecutive stereogenic axes. four dimers all have same constitutions and identical absolute...
Abstract Background and Aim: Response to interferon therapy disease progression in hepatitis C virus (HCV) infected patients differs among individuals, suggesting a possibility of contribution host genetic factors. 2′‐5′‐oligoadenylate synthetase 1 (OAS1), an important component the innate immune system with proven antiviral function, may therefore have relationship response clinical course HCV disease. Our aim was determine frequency single nucleotide polymorphism (SNP) at exon 7 splice...
A new cyclic hexapeptide, nocardiotide (1), together with three known compounds—tryptophan (2), kynurenic acid (3), and 4-amino-3-methoxy benzoic (4)—were isolated identified from the broth culture of Nocardiopsis sp. UR67 strain associated marine sponge Callyspongia Red Sea. The structure elucidation compounds were determined based on detailed spectroscopic data including 1D 2D nuclear magnetic resonance (NMR) experimental analyses in combination high resolution electrospray ionization mass...
Abstract Background and Aim: Cytomegalovirus (CMV) is a ubiquitous pathogen that infects the majority of humans. Co‐infection CMV hepatitis C virus (HCV) may deteriorate prognosis HCV‐infected patients. This study was conducted to examine role reactivation in determining response rate treatment with interferon ribavirin therapy chronic HCV Methods: Viral loads genotyping were assessed using reverse transcription polymerase chain reaction Innolipa systems, respectively. Reactivation patients...
The aim of this study was to assess the impact genetic variants oligoadenylate synthetase 1 (OAS1) single-nucleotide polymorphism (SNP) rs10774671 at exon 7 splice acceptor site on liver fibrosis progression and hepatitis C virus (HCV) outcome in Egyptian HCV genotype 4 patients. In study, 195 subjects were enrolled; 60 controls 135 chronic patients with different grades. All genotyped for OAS1 SNP by polymerase chain reaction-restriction fragment length (PCR-RFLP) analysis. There an...
Tumor necrosis factor (TNF) receptor associated factor-2 (TRAF2) knockout cells were generated to investigate the role of TRAF2 in signaling by TNFR1 and CD95-type death receptors (DR) TRAILR1/2 CD95. To prevent negative selection effects arising from increased cell sensitivity TRAF2-deficient cells, lines used for generation (KO) variants that protected DR-induced apoptosis downstream caspase-8 activation. As already described literature, KO displayed enhanced constitutive alternative NFκB...
The major complication of hepatitis C virus (HCV) infection is the induction hepatic fibrosis. In this study, we investigated correlation between expression level vascular endothelial growth factor (VEGFA) at mRNA and protein levels progression HCV-related liver One hundred twenty subjects were selected for study: 15 controls 105 chronic HCV patients with different fibrosis grades (44 F0-F1 61 F2-F4). Quantitative real-time polymerase chain reaction (qRT-PCR) was used to measure VEGFA in...
Tumor necrosis factor-α (TNFα) is a master cytokine which induces expression of chemokines and adhesion molecules, such as intercellular molecule 1 (ICAM-1) vascular cell (VCAM-1), in endothelial cells to initiate the inflammatory response. In this study, we identified neuropilin-1 (NRP1), co-receptor several structurally diverse ligands, modulator TNFα-induced response cells. NRP1 shRNA suppressed TNFα-stimulated leukocyte ICAM-1 VCAM-1 human umbilical vein (HUVECs). Likewise, it reduced...
Abstract TNF-like weak inducer of apoptosis (TWEAK) and inhibition protein synthesis with cycloheximide (CHX) sensitize for poly(I:C)-induced cell death. Notably, although CHX preferentially enhanced apoptosis, TWEAK primarily necroptosis. Both sensitizers death, however, showed no major effect on proinflammatory poly(I:C) signaling. Analysis a panel HeLa-RIPK3 variants lacking TRADD, RIPK1, FADD, or caspase-8 expression revealed furthermore similarities differences in the way how...
To evaluate the frequency of single-nucleotide polymorphism at -88 myxovirus resistance (MxA) gene promoter region in relation to status hepatitis C virus (HCV) progression and response combined interferon (IFN) chronic HCV Egyptian patients. One hundred ten subjects were enrolled study; 60 genotype 4-infected patients who underwent IFN therapy 50 healthy individuals. All genotyped for MxA by restriction fragment length technique. There was an increasing trend treatment as 34.9% GG, 64.3%...
The present study reports the synthesis of new purine bioisosteres comprising a pyrazolo[3,4-d]pyrimidine scaffold linked to mono-, di-, and trimethoxy benzylidene moieties through hydrazine linkages. First, in silico docking experiments synthesized compounds against Bax, Bcl-2, Caspase-3, Ki67, p21, p53 were performed trial rationalize observed cytotoxic activity for tested compounds. anticancer these was evaluated vitro Caco-2, A549, HT1080, Hela cell lines. Results revealed that two (5 7)...
Fibroblast growth factor (FGF)-inducible 14 (Fn14) activates the classical and alternative NFκB (nuclear ‘kappa-light-chain-enhancer’ of activated B-cells) signaling pathway but also enhances tumor necrosis (TNF)-induced cell death. Fn14 expression is upregulated in non-hematopoietic cells during tissue injury often highly expressed solid cancers. In view latter, there were are considerable preclinical efforts to target for therapy, either by exploiting as a antibodies with cytotoxic...
Most anti-CD40 antibodies show robust agonism only upon binding to FcγR
Selective TNFR2 activation can be used to treat immune pathologies by activating and expanding regulatory T-cells (Tregs) but may also restore anti-tumour immunity co-stimulating CD8