ChatGPT, powered by the GPT model and Transformer architecture, has demonstrated remarkable performance in domains of medicine healthcare, providing customized informative responses. In our study, we investigated potential ChatGPT field neurosurgery, focusing on its applications at patient, neurosurgery student/resident, neurosurgeon levels.The authors conducted inquiries with from viewpoints patients, students/residents, neurosurgeons, covering a range topics, such as disease diagnosis,...

Abstract Background With cancer-associated fibroblasts (CAFs) as the main cell type, rich myxoid stromal components in chordoma tissues may likely contribute to its development and progression. Methods Single-cell RNA sequencing (scRNA-seq), spatial transcriptomics, bulk RNA-seq, multiplexed quantitative immunofluorescence (QIF) were used dissect heterogeneity, distribution, clinical implication of CAFs chordoma. Results We sequenced here 72 097 single cells from 3 primary recurrent tumor...

Abstract Breast cancer and diabetes are significant health challenges, effective treatments for both diseases lacking. Proton pump inhibitors (PPIs) have demonstrated anticancer hypoglycemic effects, but their mechanisms of action not yet fully understood. We used the GeneCards PharmMapper databases to identify therapeutic targets diabetes, breast PPIs. identified common constructed a regulatory network drugs using STRING database Cytoscape software. also explored binding between small...

Opportunistic screening in hospitals is widely used to effectively reduce the incidence rate of cervical cancer China and other developing countries. This study aimed identify clinical risk factor algorithms that combine gynecologic examination molecular testing (paired box gene 1 (PAX1) or zinc finger protein 582 (ZNF582) methylation HPV16/18) results improve diagnostic accuracy. The delta Cp methylated PAX1 ZNF582 was obtained via quantitative methylation-specific PCR a training set (57...

Abstract Background Currently, the measurement of immune cells in previous studies is usually subjective, and no immune‐based prognostic model has been established for chordoma. In this study, we sought to simultaneously measure tumor‐infiltrating lymphocyte (TIL) subtypes chordoma samples using an objective method develop risk score (IRS) survival prediction. Methods Multiplexed quantitative immunofluorescence staining was used determine TIL levels tumoral stromal subareas 114 spinal...

Background Immunotherapy only achieves efficacy in some cancer patients, and less is known about other immune checkpoint molecules chordoma. Here, we aimed to determine the expression of PD-L1, HHLA2, B7H3, IDO-1 Galectin-9 spinal chordoma evaluated their association with tumor infiltrating lymphocytes (TILs), clinicopathological characteristics survival patients. Methods Using multiplexed quantitative immunofluorescence (QIF), simultaneously measured levels five different major TIL subsets...

NAD(P)H:quinone oxidoreductase 1 (NQO1) is overexpressed in most solid cancers, emerging as a promising target for tumor-selective killing. β-Lapachone (β-Lap), an NQO1 bioactivatable drug, exhibits significant antitumor effects on NQO1-positive cancer cells by inducing immunogenic cell death (ICD) and enhancing tumor immunogenicity. However, the interaction between β-Lap-mediated immune responses neutrophils, novel antigen-presenting (APCs), remains unknown. This study demonstrates that...

Currently, the oncogenic mechanism of endoplasmic reticulum stress-CAF (ERS-CAF) subpopulation in chordoma remains unknown. Here, single-cell RNA sequencing, spatial transcriptomics, GeoMx Digital Spatial Profiler, data-independent acquisition proteomics, bulk RNA-seq, and multiplexed quantitative immunofluorescence are used to unveil precise molecular how ERS-CAF affected progression. Results show that hypoxic microenvironment reprograms CAFs into subtype. Mechanistically, this occurrs via...

The interpretation of equivocal Papanicolaou (Pap) smear results remains challenging, even with the addition high-risk human papillomavirus test (HPV-HR). Recently, methylated zinc finger protein 582 (ZNF582) (ZNF582 (m) ) was reported to be highly associated cervical cancer. In this study, we compared performance ZNF582 detection and HPV-HR genotyping in triage atypical squamous cell undetermined significance (ASC-US) - cannot exclude a high-grade lesion (ASC-H).Two hundred forty-two...

Dihydroartemisinin (DHA), a semi-synthetic derivative of artemisinin, is known to play important roles in inhibiting proliferation rate, inducing apoptosis, as well hindering the metastasis and invasion glioma cells, but underlying mechanisms are still unclear so far. In this study, methyl thiazolyl tetrazolium (MTT), colony-forming, wound healing, invasion, apoptosis assays were performed investigate effect DHA on malignant cells. Results showed that induced cells through Protein Kinase B...

Retrospective pooled analysis of individual patient data.Spinal chondroblastoma (CB) is a very rare pathology and its clinicopathological prognostic features remain unclear. Here, we sought to characterize the data large spinal CB cohort determine factors affecting local recurrence-free survival (LRFS) overall (OS) patients.Electronic searches using Medline, Embase, Google Scholar Wanfang databases were performed identify eligible studies per predefined criteria. A retrospective review was...

Currently, the clinicopathological and prognostic characteristics of dedifferentiated chordoma (DC) poorly differentiated (PDC) remain understood. In this study, we sought to characterize parameters in a large PDC/DC cohort determine their correlations with progression-free survival (PFS) overall (OS) patients. We also attempted compare clinical features between conventional (CC).Literature searches (from inception June 01, 2020) using Medline, Embase, Google Scholar Wanfang databases were...

Breast cancer is a prevalent public health concern affecting numerous women globally and associated with palmitoylation, post-translational protein modification. Despite increasing focus on its specific implications for breast prognosis remain unclear. The work aimed to identify prognostic factors linked palmitoylation in assess effectiveness predicting responses chemotherapy immunotherapy.

BACKGROUND: Currently, little is known about the prognostic value of tumor growth rate (TGR) in spinal giant cell tumors bone (GCTB). OBJECTIVE: To investigate correlation TGR with clinicopathological features, immune microenvironment, prognosis, and response to denosumab treatment GCTB. METHODS: A total 128 patients GCTB treated at 5 centers from 2011 2021 were included. was assessed by 2 independent neuroradiologists using least preoperative thin-section magnetic resonance imaging scans a...

The 5-hydroxytryptamine-3 (5-HT₃) receptor antagonists such as ondansetron have been used to prevent and treat nausea vomiting for over 2 decades. This study was determine whether could serve a perpetrator drug causing transporter-mediated drug-drug interactions in humans. Twelve unrelated male healthy Chinese volunteers were enrolled into prospective, randomized, double-blind, crossover investigate the effects of or placebo on pharmacokinetics response metformin,...

Objective: Chondroblastoma (CB) is a rare and locally growing cartilage-derived tumor. Currently, clinical implications of tumor-associated macrophages (TAMs) in CB remain unclear. In this study, we sought to analyze the relationship between TAM parameters (including densities CD68+ CD163+ cells as well CD163+/CD68+ ratio) clinicopathological characteristics survival patients. Methods: Immunohistochemistry was used assess subtypes for CD68 CD163, expression levels p53, CD34, Ki-67 on tumor...

BackgroundMalignant pericardial effusion (MPE) is a common complication of advanced breast cancer (BRCA) and plays an important role in BRCA. This study aims to construct prognostic model based on MPE-related genes for predicting the prognosis cancer.MethodsThe BRCA samples are analyzed expression by using unsupervised cluster analysis method. processes data least absolute shrinkage selection operator multivariate Cox analysis, uses machine learning algorithms develop web tool.ResultsBRCA...

Cancer cells are characterized by increased glycolysis, known as the Warburg effect, which leads to production of cytotoxic methylglyoxal (MGO) and apoptotic cell death. often activate protective nuclear factor erythroid 2‑related factor2 (Nrf2)/glyoxalase1 (Glo1) system detoxify MGO. The effects sodium butyrate (NaB), a product gut microbiota, on Nrf2/Glos/MGO pathway underlying mechanisms in prostate cancer (PCa) were investigated present study. Treatment with NaB induced death reduced...

Background: Type 2 diabetes mellitus (T2DM) and spinal degenerative disorders (SDD) are common diseases that frequently coexist. However, both traditional observational studies recent Mendelian randomization (MR) have demonstrated conflicting evidence on the association between T2DM SDD. This comparative study explored compared SDD using MR analyses. Methods: For analyses, cross-sectional (44,972 participants with 403,095 without T2DM), case-control (38,234 409,833 SDD), prospective (35,550...

This study sought to identify molecular subtypes of breast cancer (BC) and develop a stem cells (BCSCs)-related gene risk score for predicting prognosis assessing the potential immunotherapy. Unsupervised clustering based on prognostic BCSC genes was used determine BC subtypes. Core identified by non-negative matrix factorization algorithm (NMF) were screened using weighted co-expression network analysis (WGCNA). A model constructed machine learning as well LASSO regression multivariate Cox...