A5049054329- Bone Tumor Diagnosis and Treatments
- Sarcoma Diagnosis and Treatment
- Spine and Intervertebral Disc Pathology
- Spinal Fractures and Fixation Techniques
- Oral and Maxillofacial Pathology
- Neurofibromatosis and Schwannoma Cases
- Infectious Diseases and Tuberculosis
- Cervical and Thoracic Myelopathy
- Cancer-related molecular mechanisms research
- Musculoskeletal synovial abnormalities and treatments
- Management of metastatic bone disease
- Fungal Infections and Studies
- Reproductive tract infections research
- Antifungal resistance and susceptibility
- Bacterial Infections and Vaccines
- Soft tissue tumor case studies
- Radiomics and Machine Learning in Medical Imaging
- Renal Diseases and Glomerulopathies
- Advanced Glycation End Products research
- Pelvic and Acetabular Injuries
- Bone and Joint Diseases
- Medical Imaging and Analysis
- Scoliosis diagnosis and treatment
- Cancer Immunotherapy and Biomarkers
- Parkinson's Disease and Spinal Disorders
University of South China
2020-2025
Central South University
2014-2024
First Affiliated Hospital of University of South China
2020-2024
Xiangya Hospital Central South University
2007-2024
Nanjing General Hospital of Nanjing Military Command
2023
Second Xiangya Hospital of Central South University
2014-2022
ChatGPT, powered by the GPT model and Transformer architecture, has demonstrated remarkable performance in domains of medicine healthcare, providing customized informative responses. In our study, we investigated potential ChatGPT field neurosurgery, focusing on its applications at patient, neurosurgery student/resident, neurosurgeon levels.The authors conducted inquiries with from viewpoints patients, students/residents, neurosurgeons, covering a range topics, such as disease diagnosis,...
Intervertebral disc (IVD) degeneration (IDD) is a major contributor to low back pain. During IDD progression, ROS can be produced in the form of H2 O2 nucleus pulposus cells (NPCs) response elevated cytokines, leading subsequent alternations cell fate and metabolic processes. Genetic factors are considered as main contributors pathopoiesis. Herein, we investigated detailed function mechanism H19, one most up-regulated lncRNAs specimens, -induced senescence model NPCs. H19 could accelerate...
Abstract Background With cancer-associated fibroblasts (CAFs) as the main cell type, rich myxoid stromal components in chordoma tissues may likely contribute to its development and progression. Methods Single-cell RNA sequencing (scRNA-seq), spatial transcriptomics, bulk RNA-seq, multiplexed quantitative immunofluorescence (QIF) were used dissect heterogeneity, distribution, clinical implication of CAFs chordoma. Results We sequenced here 72 097 single cells from 3 primary recurrent tumor...
Currently, the role of long noncoding RNA (lncRNA) DiGeorge syndrome critical region gene 5 (DGCR5) in intervertebral disk degeneration (IDD) remains unclear. This study explored molecular mechanisms how lncRNA DGCR5 promoted human nucleus pulposus cells (NPCs) and pyroptosis during IDD. NPCs were identified by microscopic observation, flow cytometry, immunofluorescence. An vitro NPC model was induced using lipopolysaccharides treatment. SP600125 SB203580 used to inhibit c-Jun N-terminal...
Abstract Background High-mobility group box 1 (HMGB1) is a late mediator of lethal systemic inflammation. Acute liver failure (ALF) has been shown to trigger inflammation in clinical and animal studies. To evaluate the possibility HMGB1 cytoplasmic translocation ALF, we determined whether released hepatocytes end organ patients with failure/injury. Methods HepG2 cell were stimulated LPS or TNF-α, increase extracellularly culture medium intracellularly various cellular fractions by western...
Currently, clinical implications of immune system cells in chordoma remain to be elucidated.To characterize situ cell infiltrates, the Immunoscore, and investigate their correlation with clinicopathologic data spinal patients outcome.Tumor-infiltrating lymphocytes (TILs) subtypes were assessed 54 tumor specimens using immunohistochemistry for CD3, CD4, CD8, CD20, Foxp3, PD-1, PD-L1.Overall, infiltrates present all samples there was low or moderate among several TILs subsets. PD-1+ density,...
Currently, little is known about the clinical relevance of tumor-stroma ratio (TSR) in chordoma and data discussing relationship between TSR immune status are lacking.To characterize distribution spinal chordoma, investigated its correlation with clinicopathologic or immunological features patients outcome.TSR was assessed visually on hematoxylin eosin-stained sections from 54 tumor specimens by 2 independent pathologists. Multiplex immunofluorescence used to quantify expression levels...
Abstract Background Currently, the measurement of immune cells in previous studies is usually subjective, and no immune‐based prognostic model has been established for chordoma. In this study, we sought to simultaneously measure tumor‐infiltrating lymphocyte (TIL) subtypes chordoma samples using an objective method develop risk score (IRS) survival prediction. Methods Multiplexed quantitative immunofluorescence staining was used determine TIL levels tumoral stromal subareas 114 spinal...
Background Immunotherapy only achieves efficacy in some cancer patients, and less is known about other immune checkpoint molecules chordoma. Here, we aimed to determine the expression of PD-L1, HHLA2, B7H3, IDO-1 Galectin-9 spinal chordoma evaluated their association with tumor infiltrating lymphocytes (TILs), clinicopathological characteristics survival patients. Methods Using multiplexed quantitative immunofluorescence (QIF), simultaneously measured levels five different major TIL subsets...
PURPOSE The prognostic value of lymphocyte infiltration score (LIS) and its nearest neighbor distance to tumor cells (NNDTC) in giant cell bone (GCTB) is currently not well established. This study aims characterize LIS NNDTC examine their correlation with denosumab treatment responsiveness, clinicopathologic features, patient prognosis. METHODS Using multiplexed quantitative immunofluorescence, was evaluated 253 specimens, whereas computed using HALO software. Subsequently, we analyzed the...
Currently, the oncogenic mechanism of endoplasmic reticulum stress-CAF (ERS-CAF) subpopulation in chordoma remains unknown. Here, single-cell RNA sequencing, spatial transcriptomics, GeoMx Digital Spatial Profiler, data-independent acquisition proteomics, bulk RNA-seq, and multiplexed quantitative immunofluorescence are used to unveil precise molecular how ERS-CAF affected progression. Results show that hypoxic microenvironment reprograms CAFs into subtype. Mechanistically, this occurrs via...
The exacerbation of IgA nephropathy (IgAN) is related to respiratory tract infection with hemolytic streptococcus (HS), but the mechanism unknown. In this study we investigated role chemokine ligand 20 (CCL20) in response effect T helper 17 (Th17) cells pathogenesis IgAN associated HS.Thirty mice were randomly divided into five groups: control (control), (IgAN), HS-infected (HS-IgAN), CCL20-treated (CCL20-IgAN), and HS infected (CCL20-HS-IgAN). induced lipopolysaccharide, carbon...