A5059947196- Hepatitis B Virus Studies
- Liver Disease Diagnosis and Treatment
- Hepatitis C virus research
- Helicobacter pylori-related gastroenterology studies
- Advanced Glycation End Products research
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Veterinary medicine and infectious diseases
- Alcohol Consumption and Health Effects
- Antibiotic Resistance in Bacteria
- Immune Response and Inflammation
- Microbial Metabolism and Applications
- Galectins and Cancer Biology
- Circular RNAs in diseases
- RNA modifications and cancer
- Immunotherapy and Immune Responses
- Drug-Induced Hepatotoxicity and Protection
- Pharmacological Effects of Natural Compounds
- Diabetes and associated disorders
- RNA Interference and Gene Delivery
- Cancer-related gene regulation
- Healthcare and Venom Research
- Bacteriophages and microbial interactions
- Erythrocyte Function and Pathophysiology
- Gut microbiota and health
Central South University
2014-2024
Xiangya Hospital Central South University
2012-2023
Robert Bosch (Germany)
2019-2023
Biology of Infection
2019
Engineering Associates (United States)
2019
Shanxi Agricultural University
2018
Third Xiangya Hospital
2011-2012
Hunan Provincial People's Hospital
2002
Changsha Medical University
1994-2000
Trinity College Dublin
1995
Background A liver-derived protein, fetuin-A, was first purified from calf fetal serum in 1944, but its potential role lethal systemic inflammation previously unknown. This study aims to delineate the molecular mechanisms underlying regulation of hepatic fetuin-A expression during (LSI), and investigated whether alterations levels affect animal survival, influence accumulation a late mediator, HMGB1. Methods Findings LSI induced by endotoxemia or cecal ligation puncture (CLP) knock-out...
Abstract Paclitaxel is clinically used as a first-line chemotherapeutic regimen for several cancer types, including head and neck cancers. However, acquired drug resistance results in the failure of therapy, metastasis relapse. The efflux mediated by ATP-binding cassette (ABC) transporters survival signals activated forkhead box (FOX) molecules are critical development paclitaxel resistance. Whether FOX promote through remains unknown. In this study, we developed types paclitaxel-resistant...
Emerging studies have suggested that the Hippo pathway is involved in tumorigenesis of hepatocellular carcinoma (HCC). However, key regulator liver tumor metabolic reprogramming remains elusive. Here, we provide evidence high mobility group box 1 (HMGB1), a chromosomal protein, plays role regulation during tumorigenesis. Cre/loxP recombination-mediated HMGB1 depletion hepatocytes blocks diethylnitrosamine-induced cancer initiation mice, whereas short hairpin RNA-mediated gene silencing...
The poly ADP ribose polymerase (PARP) inhibitor olaparib has been approved for treating prostate cancer (PCa) with BRCA mutations, and veliparib, another PARP inhibitor, is being tested in clinical trials. However, veliparib only showed a moderate anticancer effect, combination therapy required PCa patients. Histone deacetylase (HDAC) inhibitors have to improve the efficacy of cells, but exact mechanisms are still elusive.Several types cells epithelial cell line RWPE-1 were treated or SAHA...
The direct and indirect effects of helicobacter pylori on cell kinetics gastric epithelial line AGS were investigated by flow cytometric analysis Ki-67 positive cells MTT assay. Flow positivity permits detection that are in S-phase, whereas the assay is a colometric measure number viable cells. In absence added stimulants, 23.06 (4.88)% mean (SD) positive. When preincubated presence H pylori, there was significant increase (66.20 (7.89)%, p < 0.001). This not seen cultured Campylobacter...
To investigate the role of interleukin-8 (IL-8) and tumour necrosis factor (TNF) in patients infected with Helicobacter pylori.The study population comprised 52 dyspepsia attending for upper gastrointestinal endoscopy. Of these patients, 35 were H pylori. IL-8 TNF concentrations plasma, gastric juice, biopsy homogenate supernatant fluid measured by radioimmunoassay L929 cell bioassay, respectively.The juice homogenates substantially greater In pylori positive higher those moderate gastritis...
Despite novel advances in screening, targeting and immunotherapies, early diagnosis satisfactory treatments against hepatocellular carcinoma (HCC) remain formidable challenges. Given the unique advantages, carbon quantum dots (CQDs) become a smart theranostic nanomaterial for cancer therapy.In this work, type of bio-friendly CQDs, trichrome-tryptophan-sorbitol CQDs (TC-WS-CQDs), is synthesized from natural biocompatible tryptophan via one-pot hydrothermal method. Compared with normal...
Abstract Background Hepatocellular carcinoma (HCC) is the most common pathological type of liver cancer. Valosin-containing protein (VCP) a member AAA-ATPase family associated with multiple molecular functions and involved in tumor metastasis prognosis. However, role VCP HCC progression still unclear. Methods We examined expression using RNA sequencing microarray data from public databases measured it clinical samples cell lines by western blot, immunohistochemistry (IHC). also evaluated...
Abstract Epithelial-mesenchymal transition (EMT) is a critical step in the metastasis of hepatocellular carcinoma (HCC). BTB/POZ domain-containing protein 7 (BTBD7) regulates EMT-associated proteins implicated HCC progression. However, role(s) BTBD7 have not been identified. Using highly metastatic HCCLM3 cells, immortalized L02 hepatocytes, animal models, and three independent cohorts patient specimens, we aimed to determine involvement metastasis. We show that messenger RNA was expressed...
Abstract Oxidative stress chiefly contributes to the disruption of BBB following traumatic brain injury (TBI). The Chinese herbal medicine rhubarb is a promising antioxidant in treating TBI. Here we performed vivo and vitro experiments determine whether its absorbed bioactive compound protected after TBI by increasing ZO-1 expression through inhibition gp91 phox subunit NADPH oxidase/ROS/ERK/MMP-9 pathway. Rats were subjected controlled cortical impact (CCI) model, primary rat astrocytes...
Transmembrane 4 superfamily member 1 (TM4SF1) is a of tetraspanin family, which mediates signal transduction events regulating cell development, activation, growth and motility. Our previous studies showed that TM4SF1 highly expressed in liver cancer. HepG2 cells were transfected with TM4SFl siRNA TM4SF1-expressing plasmids their biological functions analyzed vitro vivo. overexpressing reduced apoptosis increased migration enhanced tumor metastasis vivo, whereas siRNA-mediated silencing had...
Ionizing radiation (IR) is a conventional cancer therapeutic, to which cells develop radioresistance with exposure. The residual after treatment also have increased metastatic potential. mechanisms by and gain potential are still unknown. In this study acute IR exposure induced cell senescence apoptosis, but long-term exposure, exhibited radioresistance. proliferation of radioresistant was retarded, most were arrested in G0/G1 phase. simultaneously showed resistance further IR-induced...
Abstract Therapy-induced expansion of cancer stem cells (CSCs) has been identified as one the most critical factors contributing to therapeutic resistance, but mechanisms this adaptation are not fully understood. UHRF1 is a key epigenetic regulator responsible for and controls self-renewal cells. In present study, taxane-resistant were established stem-like expanded. was overexpressed in cells, which maintained CSC characteristics. depletion overcame taxane resistance vitro vivo....
Abstract Background High-mobility group box 1 (HMGB1) is a late mediator of lethal systemic inflammation. Acute liver failure (ALF) has been shown to trigger inflammation in clinical and animal studies. To evaluate the possibility HMGB1 cytoplasmic translocation ALF, we determined whether released hepatocytes end organ patients with failure/injury. Methods HepG2 cell were stimulated LPS or TNF-α, increase extracellularly culture medium intracellularly various cellular fractions by western...
// Jia Li 1, 4 , Jiang He 2, 3 Yongming Fu 1 Xingwang Hu Lun-Quan Sun Yan Huang and Xuegong Fan Department of Infectious Diseases, Hunan Key Laboratory Viral Hepatitis, Xiangya Hospital, Central South University, Changsha 410008, China 2 Center for Molecular Medicine, Collaborative Innovation Cancer 410078, Radiation Oncology, Emergency, Correspondence to: Fan, email: xgfan57@126.com Huang, drhyan@163.com Keywords: HBV; HBx; hepatocellular carcinoma; ER stress; apoptosis Received: March 15,...
Sphingosine-1-phosphate (S1P) is a biolipid involved in chronic inflammation several inflammatory disorders. Recent studies revealed that elevated S1P contributes to sickling sickle cell disease (SCD), devastating hemolytic, genetic disorder associated with severe and tissue damage. We evaluated the effect of damage SCD underlying mechanisms. First, we demonstrated interfering receptor signaling by FTY720, U.S. Food Drug Administration-approved drug, significantly reduced systemic, local...
Abstract Hepatitis B virus-encoded X protein (HBx) acts as a tumor promoter during hepatocellular carcinoma (HCC) development, probably by regulating the expression of host proteins through protein–protein interaction. A proteomics approach was used to identify HBx-interacting involved in HBx-induced hepatocarcinogenesis. We validated findings co-immunoprecipitation and confocal microscopy. performed cell proliferation, migration assays cycle analyses HCC cells. Finally, we confirmed...
ABSTRACT Background The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has progressed to a pandemic associated with substantial morbidity and mortality. WHO the United States Center for Disease Control Prevention (CDC) have issued interim clinical guidance management of patients confirmed disease (COVID-19), but there is limited data on virologic characteristics prognosis COVID-19. Methods A total 50 COVID-19 were divided into good poor recovery groups. dynamic viral shedding...