A5001840846- Reconstructive Facial Surgery Techniques
- DNA Repair Mechanisms
- Facial Rejuvenation and Surgery Techniques
- Phytochemical Studies and Bioactivities
- Dermatologic Treatments and Research
- Cancer therapeutics and mechanisms
- Reconstructive Surgery and Microvascular Techniques
- Nasal Surgery and Airway Studies
- Bioactive Compounds and Antitumor Agents
- Cleft Lip and Palate Research
- Wound Healing and Treatments
- HIV/AIDS drug development and treatment
- Phytochemistry and Bioactive Compounds
- Ear Surgery and Otitis Media
- Marine Sponges and Natural Products
- Cancer, Hypoxia, and Metabolism
- CRISPR and Genetic Engineering
- Surgical Sutures and Adhesives
- Natural product bioactivities and synthesis
- Polyomavirus and related diseases
- Hepatitis B Virus Studies
- Craniofacial Disorders and Treatments
- Nonmelanoma Skin Cancer Studies
- RNA modifications and cancer
- Berberine and alkaloids research
West China Hospital of Sichuan University
2010-2024
Sichuan University
2015-2024
Southwest University
2024
National Natural Science Foundation of China
2014-2020
State Key Laboratory of Biotherapy
2011-2017
Kyoto University
2007-2011
West China Medical Center of Sichuan University
2011
RAD51 recombinase polymerizes at the site of double-strand breaks (DSBs) where it performs DSB repair. The loss causes extensive chromosomal breaks, leading to apoptosis. polymerization is regulated by a number mediators, such as BRCA1, BRCA2, RAD52, SFR1, SWS1, and five paralogs, including XRCC3. We here show that brca2-null mutant cells were able proliferate, indicating can perform repair in absence BRCA2. disrupted XRCC3 genes cells. All resulting double-mutant displayed phenotype was...
RAD51 is a key factor in homologous recombination (HR) and plays an essential role cellular proliferation by repairing DNA damage during replication. The assembly of at strictly controlled mediators, including BRCA1 BRCA2. We found that human directly binds GEMIN2/SIP1, protein involved spliceosome biogenesis. Biochemical analyses indicated GEMIN2 enhances the RAD51–DNA complex formation inhibiting dissociation from DNA, thereby stimulates RAD51-mediated pairing. also enhanced strand...
Deltonin, a steroidal saponin, isolated from Dioscorea zingiberensis Wright (DZW), has shown high-cytotoxic activity in cancer cells. However, its mechanisms and vivo anti-cancer effects remain unknown. In the present study, we evaluated explored anti-tumor of deltonin on panel colon cell lines mouse model murine C26. Deltonin had more cytotoxic effect C26 cells than 5-fluorouracil had, promoting dramatic G2-M phase arrest apoptosis concentration-dependent manner; oral administration...
Abstract A keloid is a benign fibroproliferative skin tumor that results from abnormal wound healing after injury and tends to grow beyond the boundary of original wound; mechanism formation still unclear. MicroRNA-21 (MiR-21) representative microRNA plays key role in variety fibrotic diseases via transforming growth factor-β/Smad signaling pathway. The aim our study was explore formation. First, we found expression miR-21 keloids fibroblasts significantly upregulated by microarray real-time...
Hyperproliferation of fibroblasts is the main cause keloid formation. However, pathogenesis keloids has yet to be fully elucidated. Tumor necrosis factor (TNF)‑α may play an important role in formation and proliferation keloids, as it implicated various fibrous disorders. In present study, expression level TNF‑α its receptors, soluble TNF receptor (sTNFR)1 sTNFR2, peripheral blood skin tissues was detected by ELISA, reverse transcription‑quantitative PCR or immunohistochemistry. There no...
Novel Janus-type nucleoside analogues (1a–d) were synthesized. Their pyrimido[4,5-d]pyrimidine base moiety has one face with a bidentate Watson–Crick donor–acceptor (DA) H-bond array of adenine and the other an acceptor–donor (AD) thymine. These nucleosides may self-associate through self-complementary pair. Indeed, in solid state, compound 6d displayed honeycomb-like supramolecular structure tetrameric membered cavities formed combination reverse pairs aromatic stacking, which solvent...
Deltonin is an active component purified from Dioscorea zingiberensis WRIGHT (DZW), and has shown anticancer effects. However, its mechanism of action remains elusive. In the present study, we investigated effect on a panel cancer cell lines analyzed in C26 cells, murine colon carcinoma cell. Our results showed that markedly inhibited growth all examined lines. induced dose- time-dependent apoptosis cells. The event was accompanied by release cytochrome c, depolarization mitochondrial...
Berberine is a natural isoquinoline alkaloid, the majority of which extracted from Huang Lian and other medicinal herbs. Numerous studies have revealed that berberine exhibits anticancer activity, however mechanisms underlying this effect remain elusive. To examine these mechanisms, we analyzed effects on panel DNA repair deficient chicken B lymphocyte (DT40) clones. Our results DT40 cells in Rev3 (Rev3-/-), translesion synthesis (TLS) gene, were hypersensitive to berberine. Following...
Abstract In keloid fibroblasts, microRNA-21 (miR-21) enhances activation of the TGF-β–Smad signaling pathway by down-regulating Smad7 expression, thereby promoting fibroblast proliferation and collagen production. However, it is unclear whether miR-21 performs above-mentioned functions through exosomal transport. Here, we extracted exosomes from culture supernatants normal skin fibroblasts observed that both types cells above secrete exosomes; however, secreted significantly more than (P...
A deficiency in the SMN gene product causes motor neuron degenerative disease spinal muscular atrophy. GEMIN2 was identified as an SMN-interacting protein, and SMN-GEMIN2 complex constitutes part of large complex, which promotes assembly spliceosomal small nuclear ribonucleoprotein (snRNP). In addition to its splicing function, we previously found that alone stimulates RAD51-mediated recombination vitro, functions DNA double-strand-break (DSB) repair through homologous vivo. However,...
Nucleoside analogues (NAs) have been the most frequently used treatment option for chronic hepatitis B patients. However, they may genotoxic potentials due to their interference with nucleic acid metabolism. Entecavir, a deoxyguanosine analog, is one of widely oral antiviral NAs against virus. It has reported that entecavir gave positive responses in both genotoxicity and carcinogenicity assays. However mechanism remains elusive. To evaluate mechanisms, we analyzed effect on panel chicken...
Abstract Novel Janus-type guanosine–cytosine pyrimido[4,5-d]pyrimidine and its 2′-deoxyribonucleoside have been synthesized in a viable route employing the Vorbruggen method.
Weak alar cartilage and lack of soft tissue on the cleft side are considered to be main critical factors leading asymmetry bilateral nostrils. The costal can provide strong structural support used maintain long-term stability nostril shape after surgical correction. With advancement in rhinoplasty techniques, application understanding lip nasal deformity is still going. Herein, we present our technique applying correct Asian patients with unilateral secondary deformity.Ninety-seven who...
The nasal ala plays an important role in the aesthetic appearance of nose. Repairing alar defect, especially full-thickness is one difficulties plastic surgeons. In this article, a new surgical method introduced about repairing defect with free auricular composite flaps and local rotation advancement tissue flaps.We retrospectively reviewed 6 patients diagnosis between 2010 2013. All them accepted method. were followed up for to 12 months. used flap form rim made shift groove region. After...
The earlobe plays an important part in the aesthetic appearance of auricle. Congenital cleft may vary considerably severity from a simple notching to extensive tissue deficiency. Most patients with require surgical correction because abnormal appearance. In this article, new technique for correcting congenital using diametric hinge flaps is introduced.We retrospectively reviewed 4 diagnosed between 2008 and 2010. All them received method. were followed up 3 6 months. attained relatively full...
Genistein (GES), a phytoestrogen, has potential chemopreventive and chemotherapeutic effects on cancer. The anticancer mechanism of GES may be related with topoisomerase II associated DNA double‐strand breaks (DSBs). However, the precise molecular remains elusive. Here, we performed genetic analyses using human lymphoblastoid TK6 cell lines to investigate whether non‐homologous end joining (NHEJ) homologous recombination (HR), two major repair pathways DSBs, were involved in repairing...