A5005351509- Microbial Natural Products and Biosynthesis
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Fungal Biology and Applications
- Marine Sponges and Natural Products
- Natural product bioactivities and synthesis
- Cancer therapeutics and mechanisms
- Natural Compound Pharmacology Studies
- Phytochemical compounds biological activities
- Phytochemical Studies and Bioactivities
- Chemical synthesis and alkaloids
- Microbial Metabolism and Applications
- 14-3-3 protein interactions
- Cancer, Hypoxia, and Metabolism
- Carbohydrate Chemistry and Synthesis
- Phytochemistry and Bioactive Compounds
- DNA and Nucleic Acid Chemistry
- Synthesis of Organic Compounds
- Alkaloids: synthesis and pharmacology
- Genomics and Phylogenetic Studies
- Signaling Pathways in Disease
- Biological Activity of Diterpenoids and Biflavonoids
- Genetic Mapping and Diversity in Plants and Animals
- Cancer Treatment and Pharmacology
- Plant Pathogens and Fungal Diseases
Huazhong University of Science and Technology
2016-2025
New York University
2024-2025
Tongji Hospital
2015
Sichuan University
2011-2014
State Key Laboratory of Biotherapy
2011-2012
Ampelopsis grossedentata, an indigenous plant in southern China, has been used for treating pharyngitis traditional Chinese medicine hundreds of years. In this study, we explored the anti-inflammatory activity dihydromyricetin (1), its major bioactive component, and underlying mechanism action. We demonstrated that 1 suppressed levels pro-inflammatory cytokines such as tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6) well increased level cytokine interleukin-10...
Abstract Asperchalasine A ( 1 ), the first cytochalasan dimer featuring a unique decacyclic 5/6/11/5/5/6/5/11/6/5 ring system consisting of 20 chiral centers, was isolated from culture broth Aspergillus flavipes . Three biogenetically related intermediates, asperchalasines B–D 2 – 4 were also isolated. Their structures, including their absolute configurations, elucidated using combination HRESIMS, NMR, ECD, molecular modeling, and single‐crystal X‐ray diffraction techniques. Compound , which...
Abstract Two bioactive merocytochalasans, epicochalasines A ( 1 ) and B 2 ), a new class of cytochalasans bearing unexpected scaffolds consisting fused aspochalasin epicoccine dimer moieties, were isolated from the liquid culture broth Aspergillus flavipes . Both possess hendecacyclic 5/6/11/5/6/5/6/5/6/6/5 ring system containing an adamantyl cage as many 19 stereogenic centers; however, fusion patterns differ greatly, thus resulting in different carbon skeletons. The absolute configurations...
Abstract Asperflavipines A ( 1 ) and B 2 ), two structurally complex merocytochalasans, were isolated from Aspergillus flavipes . Asperflavipine which contains cytochalasan moieties epicoccine moieties, is the first heterotetramer to be discovered. It uniquely defined by 5/6/11/5/6/5/6/5/6/5/5/11/6/5 fused tetradecacyclic rings with three continuous bridged ring systems. a heterotrimer containing 5/6/11/5/5/6/5/6/5 nonacyclic system. The hypothetical biosynthesis of proposed involve...
Armochaeglobines A (1) and B (2), two indole-based cytochalasan alkaloids with new carbon skeletons, were obtained from the fungus Chaetomium globosum TW1-1, which was first isolated arthropod Armadillidium vulgare. Their structures elucidated by extensive spectroscopic analyses, ECD calculation, single-crystal X-ray diffraction analysis. Interestingly, compound 1 featured a unique tetracyclic 5/6/7/5 fused ring system 2 possessed rare 12-membered scaffold.
Bipoladiens A–E (1–5), five new ophiobolin-derived sesterterpenoids, and a known compound 6 (bipolaricin R) were isolated from the cultures of phytopathogenic fungus Bipolaris maydis. Their structures absolute configurations elucidated based on comprehensive spectroscopic analyses, HRESIMS, electronic circular dichroism (ECD) calculations, single-crystal X-ray diffraction analyses. Notably, 1 has an undescribed tetracyclic 5/8/5/7 fused carbon skeleton, 2 possesses rare multicyclic caged...
G-quadruplex (G4) selective stabilizing ligands can regulate c-MYC gene expression, but the kinetic basis remains unclear. Determining effects of on promoter G4s' folding/unfolding kinetics is challenging due to polymorphic nature G4s and high energy barrier unfold G4s. Here, we used single-molecule magnetic tweezers manipulate a duplex hairpin containing sequence mimic transiently denatured during transcription. We measured six commonly binding competition between quadruplex structures, as...
The new polyprenylated acylphloroglucinol derivatives 1–15 and the known furohyperforin (16) were isolated from stems leaves of Hypericum perforatum. Their structures determined by analyses NMR HRESIMS data. absolute configurations elucidated a combination electronic circular dichroism (ECD) Rh2(OCOCF3)4-induced ECD, as well X-ray diffraction crystallography. hyperforatin F (9) contains unique acetyl functionality at C-1 bicyclo[3.3.1]nonane core. Hyperforatins G (10) H (11) are similarly...
Aspergilasines A–D (1–4), four merocytochalasans with new skeletons, were isolated from the liquid culture broth of Aspergillus flavipes QCS12. Aspergilasine A (1) possesses a uniquely caged pentacyclo[7.2.0.14,11.02,7.05,10]dodecane skeleton an unexpected cyclobutane ring that formed by fusion two epicoccine moieties via [3 + 2] cycloadditions. B–D (2–4) are key biosynthetic intermediates epicochalasine B fit well our previous proposal.
Zephycandidine A (1), the first naturally occurring imidazo[1,2-f]phenanthridine alkaloid, was isolated from Zephyranthes candida (Amaryllidaceae). The structure of 1 elucidated by spectroscopic analyses and NMR calculation, a plausible biogenetic pathway for zephycandidine (1) proposed. exhibited significant cytotoxicity against five cancer cell lines with IC50 values ranging 1.98 to 7.03 μM selectivity indices as high 10 when compared normal Beas-2B cell. Further studies suggested that...
Distinctive from their normal counterparts, cancer cells exhibit unique metabolic dependencies on glutamine to fuel anabolic processes. Specifically, pancreatic ductal adenocarcinoma (PDAC) rely an unconventional pathway catalyzed by aspartate transaminase 1 (GOT1) rewire metabolism and support nicotinamide adenine dinucleotide phosphate (NADPH) production. Thus, the important role of GOT1 in energy Reactive Oxygen Species (ROS) balance demonstrates that targeting may serve as therapeutic...
Introduction Psoriasis is a common inflammatory skin disease recognized by the World Health Organization as "an incurable chronic, noninfectious, painful, disfiguring and disabling disease." The fact that metabolic syndrome (MetS) most important comorbidities of psoriasis suggests an role lipid metabolism in pathogenesis psoriasis. Narciclasine (Ncs) alkaloid isolated from Amaryllidaceae plants. Its biological activities include antitumor, antibacterial, antiinflammatory, anti-angiogenic...
Abstract Chronic kidney disease (CKD) is a that affects more than 850 million people. Acute injury (AKI) common cause of CKD, and blocking the AKI–CKD transition shows promising therapeutic potential. Herein, we found butyrolactone I (BLI), natural product, exerts significant nephroprotective effects, including maintenance function, inhibition inflammatory response, prevention fibrosis, in both folic acid‐ ureteral obstruction‐induced mouse models. Notably, BLI showed greater blood urea...
Abstract The contributions of genetic interactions to natural trait variation are challenging estimate experimentally, as current approaches for detecting epistasis often underpowered. Powerful mapping such bulk segregant analysis, wherein individuals with extreme phenotypes pooled genotyping, obscure by averaging over genotype combinations. To accurately characterize and quantify underlying variation, we have engineered strains the budding yeast Saccharomyces cerevisiae enable crosses where...
Cytochathiazines A–C (1–3), which represent a new type of merocytochalasan, were isolated from coculture Chaetomium globosum and Aspergillus flavipes. Compounds 1–3 are the first natural products featuring an unprecedented 2H-1,4-thiazine functionality. Plausible biosynthetic pathways for with chaetoglobosin dipeptide as main constitutional units proposed. Compound 2 induced apoptosis in leukemia cells through activation caspase-3 degradation poly ADP-ribose polymerase.
Diketopiperazines are important secondary metabolites of the fungi with variety bioactivities. Several species belonging to genus Chaetomium produce compounds this class, such as chetomin. To identify new antitumor agents, fungus sp 88194 were investigated and three indole diketopiperazines, Chaetocochins G (1), Oidioperazines E (2) Chetoseminudin (3), along two known Chetoseminudins C (4) N-acetyl-β-oxotryptamine (5), obtained. recrystallized in CHCl3 containing a small amount MeOH, their...