A5058640231- CAR-T cell therapy research
- Lymphoma Diagnosis and Treatment
- Pluripotent Stem Cells Research
- Monoclonal and Polyclonal Antibodies Research
- CRISPR and Genetic Engineering
- Asthma and respiratory diseases
- Advancements in Semiconductor Devices and Circuit Design
- Chronic Lymphocytic Leukemia Research
- Mast cells and histamine
- Neurogenesis and neuroplasticity mechanisms
- Viral-associated cancers and disorders
- Urticaria and Related Conditions
- Immunotherapy and Immune Responses
- Nanowire Synthesis and Applications
- HER2/EGFR in Cancer Research
- Renal and related cancers
- Virus-based gene therapy research
- Urological Disorders and Treatments
- RNA Interference and Gene Delivery
- Connexins and lens biology
- Ovarian cancer diagnosis and treatment
- Hereditary Neurological Disorders
- Salivary Gland Disorders and Functions
- Anorectal Disease Treatments and Outcomes
- Genomic variations and chromosomal abnormalities
Chinese Institute for Brain Research
2018-2024
Capital Medical University
2014-2024
Binzhou University
2019-2024
Binzhou Medical University
2019-2024
Ministry of Education of the People's Republic of China
2014
University of Nebraska Medical Center
2006-2012
University of Oxford
2009
University of Oslo
2009
John Radcliffe Hospital
2009
CHI Health Creighton University Medical Center - Bergan Mercy
2005-2007
Abstract Purpose: Hans and coworkers previously developed an immunohistochemical algorithm with ∼80% concordance the gene expression profiling (GEP) classification of diffuse large B-cell lymphoma (DLBCL) into germinal center B-cell–like (GCB) activated (ABC) subtypes. Since then, new antibodies specific to B-cells have been developed, which might improve performance immunostain algorithm. Experimental Design: We studied 84 cases cyclophosphamide-doxorubicin-vincristine-prednisone...
Abstract It remains a challenge to differentiate human induced pluripotent stem cells (iPSCs) or embryonic (ES) Purkinje cells. In this study, we derived iPSCs from fibroblasts and directed the specification of first progenitors, by adding Fgf2 insulin embryoid bodies (EBs) in time-sensitive manner, which activates endogenous production Wnt1 Fgf8 EBs that further patterned towards midbrain-hindbrain-boundary tissue identity. Neph3-positive progenitors were sorted out using flow cytometry...
Glioblastoma (GBM) is a common primary brain tumor with poor clinical prognosis. Although CAR-T therapy has been trialed for treatment of GBM, the outcomes are sub-optimal possibly due to exhaustion T cells and life-threatening neurotoxicity. To address these issues, combined therapeutic strategy was tested in current study using GD2 together Nivolumab - an anti-PD-1 monoclonal antibody. An effector-to-target co-culture system established evaluate short-term long-term cytotoxicity CAR-T, as...
CD19 chimeric antigen receptor (CAR)-T therapy has shown impactful results in treatment of B-cell malignancies. However, immune recognition the murine scFv may render subsequent infusion(s) ineffective. Also, nonselective expansion both CAR-transduced and nontransduced T cells during production stage affects yield purity final products. Here, we aim to develop a humanized selective (hs) CAR solve above problems.Experimental Design: A hsCAR was designed, which incorporated short domain...
Lmx1a plays a central role in the specification of dopaminergic (DA) neurons, which potentially could be employed as key factor for trans-differentiation to DA neurons. In our previous study, we have converted somatic cells directly into neural stem cell—like cells, namely induced (iNSCs), further can differentiated subtypes neurons and glia vitro. present continued test whether these iNSCs therapeutic effects when transplanted mouse model Parkinson's disease (PD), especially was introduced...
HIV-infected individuals have a significantly increased risk of developing an aggressive B-cell Non-Hodgkin Lymphoma relative to HIV(−) persons. Due their nature, AIDS-related lymphomas (ARL) can also be more difficult classify. Genetic abnormalities are known play significant role in lymphomagenesis. To aid case classification and identify key pathogenetic events ARL, we analyzed gene expression data somatic DNA copy number changes by high-resolution array comparative genomic hybridization...
The normal counterparts of mantle cell lymphoma (MCL) are naive, quiescent B cells that have not been processed through the germinal center (GC). For this reason, although lymphomas arising from GC or post-GC often exhibit plasmacytic differentiation, MCL rarely presents with features. Seven cases a monotypic plasma (PC) population were collected 6 centers and studied by immunohistochemistry, fluorescence immunophenotyping interphase cytogenetics as tool for investigation neoplasms analysis,...
The use of small interfering RNAs (siRNAs) to silence target gene expression has greatly facilitated mammalian genetic analysis by generating loss-of-function mutants. In recent years, high-throughput, genome-wide screening siRNA libraries emerged as a viable approach. Two different methods have been used generate short hairpin RNA (shRNA) libraries; one is chemically synthesized oligonucleotides, and the other convert complementary DNAs (cDNAs) into shRNA cassettes enzymatically. high cost...
The molecular mediators underlying the effects of inflammation on neural stem cells (NSCs) are not fully characterized. In this study, we identified Ascl2 as a downstream basic helix-loop-helix (bHLH) transcription factor in NSCs following exposure to TNFα. Under normal conditions, expression is inhibited at post-transcriptional levels by miR-26a, which targets 3' untranslated region (UTR) Ascl2. Upon TNFα, miR-26a reduced, leads up-regulation Overexpression promotes neuronal...
Abstract The induced pluripotent stem cell (iPSC) technology has provided a unique opportunity to develop disease-specific models and personalized treatment for genetic disorders, is well suitable the study of Werner syndrome (WS), an autosomal recessive disease with adult onset premature aging caused by mutations in RecQ like helicase (WRN) gene. WS-derived fibroblasts were previously shown be able generate iPSCs; however, it remains elusive how iPSCs behave whether they are mimic...
We aimed to quantify the extent of salivary gland fibrosis using shear-wave elastography (SWE) assess its diagnostic value for primary Sjögren syndrome (pSS).A total 58 pSS patients and 44 controls underwent SWE ultrasound evaluation parotid submandibular glands. measured degree in all participants investigated accuracy relationship disease progression.The sensitivity, specificity, were highest when critical Young's modulus values glands 18.4 15.9 kPa, respectively, effectively improving...
Murine-based CD19 CAR-T (CD19m CAR-T) therapy can lead to a relatively high CR rate when administered B-ALL patients for the first time. However, DOR is sub-optimal and subset of even show primary resistance CD19m CAR-T. To address these issues, we employed humanized selective CD19CAR-T (CD19hs evaluated long-term safety efficacy treating 8 R/R who had relapsed or failed achieve following infusion (Clinical trials' number: ChiCTR1800014761 ChiCTR1800017439). Of patients, 7 achieved on Day 30...
To determine the functional abnormalities of Leu89Pro mutation in connexin32 (CX32), which we have previously reported is present within an X-linked dominant Charcot-Marie-Tooth disease family. In this family, male patients were moderately to severely affected.We performed immunofluorescence investigate whether CX32 protein was transported cell membrane HeLa and Schwann cells. First, constructed eukaryotic express plasmids expressing (wild-type or Leu89Pro) enhanced green fluorescent by gene...
CD19 chimeric antigen receptor T cell (CD19CAR-T) has shown great potential to treat acute B lymphoblastic leukemia (B-ALL) and lymphoma, most of anti-CD19 scFv are derived from murine antibody sequences. However, about 10-20% B-ALL patients exhibit primary resistance murine-based CD19CAR-T (CD19mCAR-T). Herein, we report that a humanized selective (CD19hsCAR-T) may offer solution this problem.
不知火姫菊乾燥花弁を生理リン酸緩衝液(PBS)もしくはエタノールで抽出し,各種癌細胞株の増殖に及ぼす影響について検討した.いずれの抽出液も330nmに吸収極大を与え,PBS抽出液がより高い吸光度を与えた.ラット肝癌細胞株dRLh-84の培養液に10分の1量のPBS抽出液もしくは1000分の1量のエタノール抽出液を添加して4日間培養した結果,細胞増殖抑制効果はPBS抽出液にのみ認められた.PBS抽出液はラット正常肝細胞株RLN-10に対してはdRLh-84細胞より弱い増殖抑制効果を示した.各種ヒト癌細胞株に対するPBS抽出液の増殖抑制効果を比較した結果,本抽出液は肝癌細胞株HuH-7およびHepG2に対して強い毒性を示し,乳癌細胞株MCF-7にも致死作用を示したが,白血病細胞株K562およびHL-60に対しては致死効果がほとんど認められず,肺癌細胞株に対しては増殖抑制効果のみ示すことが明らかとなった.これらの結果は乾燥菊花弁抽出物の細胞毒性が癌細胞の由来により異なることを示している.
The airn of this study was to investigate the effect time day on secretory immunoglobulin A (slgA).Six males participated in study.Saliva samples were collected for each subject at 06:OO, 06:30.07:OO, 07:30, 11:30, 18:OO, 22:30 and 06:OO next morning both routine diet prescribed day.SlgA concentration determined by an enzyme linked irnmunosorbent assay, total saliva protein BCA kit.There no significant diurnal variation flow rate secretion high low afternoon evening days, levels slgA 18:OO...