Abstract Background and Objectives Increased rigidity of the extracellular matrix (ECM) is commonly associated with hepatocellular carcinoma (HCC). The purpose this study was to quantitate mechanical stiffness various hepatic tissues, evaluate integrin β1 expression, investigate correlation between these two factors in development HCC. Methods Twenty‐three normal specimens, 152 cases cirrhosis, 105 HCC were included study. ECM each specimen detected using atomic force microscopy calculate...

Background: Long noncoding RNAs (lncRNAs) are vital mediators in human cancers including pituitary neuroendocrine tumor (PitNET) and could function as competing endogenous (ceRNAs) of microRNAs (miRNAs). The main objective this study is to identify effect lncRNA X-inactive specific transcript (XIST) microRNA-424-5p (miR-424-5p) on PitNET. Methods: Microarray analysis was employed the PitNET-related differentially expressed lncRNAs. PitNET tissues, both invasive non-invasive subtypes parallel...

The use of chelators for cancer treatment has been an alternative option. Dithiocarbamates have recently attracted considerable attention owning to their diverse biological activities; thus, the preparation new dithiocarbamate derivatives with improved antitumor activity and selectivity as well probing underlying molecular mechanism are required. In this study, di-2-pyridylhydrazone S-propionic acid (DpdtpA) its copper complex were prepared characterized, antiproliferative was evaluated....

The Mannich base containing ciprofloxacin and kojic acid structural units was prepared evaluated in antitumor activity. enhancement activity observed both from the (IC(50): 103.3±5.0 µM for HepG2, 87.9±8.0 HCT-116 cell) its copper complex 11.5±1.8 44.4±2.5 compared to acid. mechanistic studies via RT-PCR, cell cycle analysis, mitochondrial membrane potential measurement, inhibition of topoisomerase molecular docking indicated that there is a different mechanism between complex. cytotoxicity...

Cyclin-dependent kinases (CDKs) are cell cycle regulators, and abnormal activation can accelerate tumor proliferation. However, The relation between CDKs dysregulation to colorectal cancer incidence progression have not been examined in detail. Methods:Differences expression normal tissues, associations clinical prognosis, frequencies of gene mutations, the influences on infiltration by immune cells were analyses Oncomine, Gene Expression Profiling Interactive Analysis, Kaplan-Meier plotter,...

Iron depletion and stimulation of iron-dependent free radical damage is a rapidly developing field for chelation therapy, but the iron mobilization from ferritin by chelators has received less attention. In this study, di-2-pyridylketone 2-pyridine carboxylic acid hydrazone (DPPCAH) its copper complex was prepared characterized NMR MS spectra. The proliferation inhibition assay showed that both DPPCAH exhibited selectively HepG2 (IC50, 4.6±0.2 µM DPPACH 1.3±0.2 complex), HCT-116 cell line...

Cancer cells have higher demand of iron and copper ions for growth, disturbing the metal's homeostasis can inhibit proliferation cancer cell. Dithiocarbamates possessing excellent metal chelating ability antitumor activity are considered as candidates in chelation therapy, however, their molecular mechanisms remain to be elucidated. In present study, a dithiocarbamate derivative, di-2-pyridylhydrazone s-acetic acid (DpdtaA) was prepared address issue whether mechanism behind biological...

The senescence of vascular endothelial cells (VECs) drives the occurrence and development cardiovascular disease (CVD). Homocysteine (HCY) is a general risk factor for age-associated CVDs. Autophagy, an evolutionarily conserved lysosomal protein degradation pathway, serves part in VEC senescence. purpose this study was to investigate role autophagy HCY-induced cell explore novel mechanisms therapeutic approaches related Human umbilical vein (HUVECs) were isolated from fresh cords healthy...

Reactive oxygen species (ROS) production is involved in the mechanism of action a number drugs, but biological effects ROS remain to be clarified. Furthermore, ferroptosis involves iron-dependent that may derived from ferritinophagy; however, association between and ferritinophagy has not been fully established. The present study demonstrated dithiocarbamate derivatives (iron chelators) exhibited antineoplastic properties involving induction, whether underlying mechanisms was unknown. To...

This paper describes an assessment of the Impact weather changes and human visitation on behavior activity level Humboldt penguin (Spheniscus humboldti) in captivity.The study is based time-budgets from a flock over 20 birds at Fota Wildlife Park, Cork, Ireland, gathered during daytime observations six month period.The results show that penguins spent most stationary, terrestrial locomotion, preening swimming modes behavior, increased rainy days.Peak took place 16:00 to 17:00 hours,...

Ferritinophagy is a process of ferritin degradation in lysosomes; however, how its effect on other cellular events, such as epithelial-mesenchymal transition (EMT) and ferroptosis remains elusive. In this study, we determined ferritinophagic flux influence the status EMT HepG2 cell. Our data revealed that 2-pyridylhydrazone dithiocarbamate s-acetic acid (PdtaA) induced inhibition involved ferritinophagy-mediated ROS production, but addition ferrostatin-1 could attenuate PdtaA regulation...

Abstract The growth of cancer, the effectiveness treatment, and prognosis are all closely related to PANoptosis (include pyroptosis, apoptosis, necroptosis). It remains unclear whether genes (PANGs) may contribute lower-grade glioma tumor microenvironment (TME). In this study, Firstly, we provided a comprehensive review pan-cancer landscape PANGs in terms expression characteristics, prognostic value, mutational profile, pathway regulation. Then, identified two distinct PAN clusters 611 LGG...

Objective To investigate the expression of integrin beta 1 in hepatic cirrhosis (HC) and hepatocellular carcinoma (HCC). Methods The HCC, HC normal liver tissues was detected by reverse transcriptase-polymerase chain reaction (RT-PCR) laser scanning confocal microscopy (LSCM). association between clinical pathological features were analyzed. Results (1) levels mRNA protein HCC (1.30 ±0.24, 90.50 ± 33.50) (1.58 0.31, 123.10 38.90) much higher than that tissue (0.37 0.08, 11.90 6.00) (P ＜...

Cultivated fields and forests in South China are experiencing serious damage due to invasive alien plants. We investigated the relation between DNA C-values invasiveness. The of 23 species ranged from 0.39 pg 3.37 pg. Herbs, perennials native had higher mean than shrubs, annuals species. decreased with increasing Paederia scandens, a harmful species, has lowest C-value among perennials, indicating that low nuclear content may also possess an potential.

Objective To investigate the predictive factors of short-term poor outcome in patients with cerebral venous sinus thrombosis （CVST）. Methods The clinical data 42 consecutive inpatients CVST were analyzed retrospectively. outcomes assessed modified Rankin scale （mRS） at discharge. divided into either a good group （mRS 0 to 2） or 3 6）. related factors, such as demographic, etiology, and features compared between two groups, Multivariate logistic regression analysis was used determine...

Objective To investigate the expression of integrinβ1 in different parts hepatocel-lular carcinoma( HCC) and its clinical significance. Methods Integrinβ1 distribution were determined HCC, adjacent tissues distant by reverse transcriptase -polymerase chain reaction(RT-PCR) laser scanning confocal microscopy (LSCM) immunoflurometric assay.The relationship between clinic-pathologic features was analyzed accordingly. Results The positively correlated to degree hepatic cirrhosis. overexpression...