A5101895615- Animal Virus Infections Studies
- Aquaculture disease management and microbiota
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- Pancreatic and Hepatic Oncology Research
- Energy Efficient Wireless Sensor Networks
- Insect Resistance and Genetics
- Plant Virus Research Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Circular RNAs in diseases
- Mosquito-borne diseases and control
- Heat shock proteins research
- Cellular Mechanics and Interactions
- Security in Wireless Sensor Networks
- Radioactive contamination and transfer
- Luminescence and Fluorescent Materials
- Nuclear Materials and Properties
- Radioactive element chemistry and processing
- Photodynamic Therapy Research Studies
- Autophagy in Disease and Therapy
- Nanoplatforms for cancer theranostics
- Viral gastroenteritis research and epidemiology
China Academy of Engineering Physics
2023-2024
Weifang Medical University
2024
Hechi University
2024
Huazhong University of Science and Technology
2010-2022
Union Hospital
2009-2022
China Three Gorges University
2020
State Key Laboratory of Silkworm Genomic Biology
2018-2019
Southwest University
2018-2019
South China Sea Institute Of Oceanology
2013-2018
Institute of Oceanology
2013-2018
Here we report the complete genome sequence of Singapore grouper iridovirus (SGIV). Sequencing random shotgun and restriction endonuclease genomic libraries showed that entire SGIV consists 140,131 nucleotide bp. One hundred sixty-two open reading frames (ORFs) from sense antisense DNA strands, coding for lengths varying 41 to 1,268 amino acids, were identified. Computer-assisted analyses deduced acid sequences revealed 77 ORFs exhibited homologies known virus genes, 23 which matched...
miRNAs are associated with various types of cancer due to their ability affect expression genes that modulate tumorigenesis. In this study, we explored the role miR-141 in pancreatic cancer. The analysis clinical characteristics showed was significantly downregulated tissues and cell lines Moreover, decreased level tumor size TNM stage, as well lymph node distant metastasis. Meanwhile, both Kaplan-Meier multivariate survival were overall survival. Overexpression cells inhibited...
Accumulating evidence indicates that microRNAs (miRNAs) are aberrantly expressed in human cancer and contribute to the tumorigenesis, but their roles pancreatic still largely unknown. In this study, our data showed miR-130b was significantly downregulated 52 pairs of tissues five cell lines. Furthermore, deregulated correlated with worse prognosis, increased tumor size, late TNM stage, lymphatic invasion distant metastasis. Multivariate analysis expression a significant independent...
Abstract miRNAs are small noncoding RNAs that participate in a variety of biologic processes, and dysregulation miRNA is always associated with cancer development progression. Aberrant expression miR-148b has been found some types cancer, but its potential role pancreatic still largely unknown. In this study, our data showed was significantly downregulated 48 pairs human tissues five cell lines. Furthermore, the deregulated correlated increased tumor size, late tumor–node–metastasis stage,...
Epithelial-mesenchymal transition (EMT) is supposed to be an inflammation induced response which may take a central role in tumorigenesis. Since recent evidence indicates that microRNAs involved EMT, the present study set out reveal miRNA might regulate EMT CP (chronic pancreatitis) and PC (pancreatic cancer) its potential mechanism. Firstly, we provided both tissues demonstrated active profile. Consistently, miR-217 was obviously down-regulated CP, TGF-β1 treated cells, while negatively...
Abstract Background and Objectives Increased rigidity of the extracellular matrix (ECM) is commonly associated with hepatocellular carcinoma (HCC). The purpose this study was to quantitate mechanical stiffness various hepatic tissues, evaluate integrin β1 expression, investigate correlation between these two factors in development HCC. Methods Twenty‐three normal specimens, 152 cases cirrhosis, 105 HCC were included study. ECM each specimen detected using atomic force microscopy calculate...
Grouper Epinephelus spp. is one of the most important mariculture fish species in China and South-East Asian countries. The emerging viral diseases, evoked by iridovirus which belongs to genus Megalocytivirus Ranavirus, have been well characterized recent years. To date, few data on lymphocystis disease grouper caused virus (LCDV) were described. Here, a novel LCDV isolate was identified characterized. Based sequence major capsid protein (MCP) DNA polymerase gene, we found that causative...
Abstract Cancer cells predominantly produce energy at a high rate of glycolysis even in aerobic environment. It is termed as Warburg effect and necessary for the tumorigenesis. Studies showed pyruvate kinase M2 (PKM2), key regulator effect, overexpressed involved numerous cancers. However, expression function PKM2 pancreatic cancer (PC) remain undefined. Our results that PC tissue compared to peritumoral tissue. Unexpected, downregulation did not affect proliferation, invasion,...
Photodynamic therapy (PDT) has emerged as a promising locoregional of hepatocellular carcinoma (HCC). The utilization luminogens with aggregation-induced emission (AIE) characteristics provides new opportunity to design functional photosensitizers (PS). PSs targeting the critical organelles that are susceptible reactive oxygen species damage is strategy enhance effectiveness PDT. In this paper, PS, 1-[2-hydroxyethyl]-4-[4-(1,2,2-triphenylvinyl)styryl]pyridinium bromide (TPE-Py-OH)...
The aim of this paper was to develop a loop-mediated isothermal amplification (LAMP) method for rapid, sensitive and inexpensive detection Singapore grouper iridovirus (SGIV) in (GP), Epinephelus sp.A set six specific primers designed by targeting the SGIV ORF-014L. With Bst DNA polymerase large fragment, target can be amplified as early 20 min at 65 degrees C simple water bath. limit is about 0.02 fg (equivalent 6.3 copies) plasmid LAMP products could judged with three different methods....
Abstract We developed a highly sensitive method to detect liver tissue stiffness with atomic force microscopy (AFM), and investigated the physical features of hepatocarcinogenesis. Wistar rats received weekly intraperitoneal injections diethylnitrosamine (DEN) or saline (control) followed by 2‐week wash‐out period. Liver samples were harvested at 10, 14, 18 weeks for pathological examination stress detection. Previously normal tissues fibrosis carcinoma after DEN administration. Although...
Abstract The CRISPR/Cas9 system is a powerful tool for the treatment of infectious diseases. In our previous study, we knocked out Bombyx mori nucleopolyhedrovirus (BmNPV) key genes and BmNPV‐dependent host factor to generate transgenic antiviral strains. To further expand range target BmNPV more effectively prevent control pathogenic infections, performed gene editing analysis by constructing target‐directed baculovirus early transcriptional activator immediate early‐0 ( ie‐0 ) 2 ie‐2...
A new brain‐cell line, EMB, was developed from kelp grouper Epinephelus moara , a cultured marine fish. The EMB cells were subcultured for more than 60 passages. in Leibovitz's L‐15 medium (L15) supplemented with antibiotics, foetal bovine serum (FBS), 2‐mercaptoethanol (2‐ME) and basic fibroblast growth factor (bFGF). could grow at 18–30° C, the maximum between 24 30° C. optimum FBS concentration ranged 15 20%. Chromosome analysis indicated that modal chromosome number 48 passage 45. After...