A5048323867- Pancreatic function and diabetes
- Diabetes and associated disorders
- Diabetes Management and Research
- Diabetes Treatment and Management
- RNA Research and Splicing
- Viral gastroenteritis research and epidemiology
- RNA modifications and cancer
- Gut microbiota and health
- Metabolism, Diabetes, and Cancer
- Mindfulness and Compassion Interventions
- Chemotherapy-induced cardiotoxicity and mitigation
- Protein Structure and Dynamics
- Asthma and respiratory diseases
- Student Stress and Coping
- RNA and protein synthesis mechanisms
- Cuban History and Society
- Race, History, and American Society
- Child and Adolescent Psychosocial and Emotional Development
- Hippo pathway signaling and YAP/TAZ
- Pancreatitis Pathology and Treatment
- Cinema and Media Studies
- Bipolar Disorder and Treatment
Vanderbilt University
2020-2024
Vanderbilt University Medical Center
2024
Princeton University
2016-2019
Nashville Oncology Associates
2018-2019
Radford University
2014
The nucleolus is a prominent nuclear condensate that plays central role in ribosome biogenesis by facilitating the transcription and processing of nascent ribosomal RNA (rRNA). A number studies have highlighted active viscoelastic nature nucleolus, whose material properties phase behavior are consequence underlying molecular interactions. However, ways which impact its function rRNA not understood. Here we utilize Cry2olig optogenetic system to modulate nucleolus. We show above threshold...
Endogenous β cell regeneration could alleviate diabetes, but proliferative stimuli within the islet microenvironment are incompletely understood. We previously found that recovery following hypervascularization-induced loss involves interactions with endothelial cells (ECs) and macrophages (MΦs). Here we show ECs modulate MΦ infiltration phenotype during loss, recruited MΦs essential for recovery. Furthermore, VEGFR2 inactivation in quiescent accelerates vascular regression leads to...
The autonomic nervous system regulates pancreatic function. Islet capillaries are essential for the extension of axonal projections into islets, and both these structures important appropriate islet hormone secretion. Because beta cells provide paracrine cues glucagon secretion neurovascular development, we postulated that cell loss in type 1 diabetes (T1D) would lead to a decline intraislet reduction innervation, possibly contributing abnormal To define morphological characteristics nerve...
A variety of islet autoantibodies (AAbs) can predict and possibly dictate eventual type 1 diabetes (T1D) diagnosis. Upwards 75% those with T1D are positive for AAbs against glutamic acid decarboxylase (GAD65 or GAD), a producer gamma-aminobutyric (GABA) in human pancreatic beta cells. Interestingly, bacterial populations within the gut also express GAD produce GABA. Evidence suggests that dysbiosis microbiome may correlate pathogenesis physiology. Therefore, autoimmune linkages between...
Exposure to chemotherapeutic agents has been linked an increased risk of type 2 diabetes (T2D), a disease characterized by both the peripheral insulin resistance and impaired glucose-stimulated secretion (GSIS) from pancreatic β-cells. Using rat β-cell line INS-1 832/13 isolated mouse islets, we investigated effect drug doxorubicin (Adriamycin) on survival function. cells caused impairment GSIS, cellular viability, increase in toxicity, as soon 6 h post-exposure. Doxorubicin plasma membrane...
Narasimhan, Snehaa; Hodge, Rachela; Doyle, Glenn A.a; Kraemer, David J.M.d; Prabhakaran, Ranjanid; Rickels, Karlc; Richardson, Tiffanyc; Bloch, Paul J.a; Lohoff, Falk W.a,b,c Author Information
Abstract A variety of islet autoantibodies (AAbs) can predict and possibly dictate eventual type 1 diabetes (T1D) diagnosis. Upwards 75% those with T1D are positive for AAbs against glutamic acid decarboxylase (GAD65), a producer gamma-aminobutyric (GABA) in human pancreatic beta cells. Interestingly, bacterial populations within the gut also express GAD65 produce GABA. Evidence suggests that dysbiosis microbiome may correlate pathogenesis physiology. Therefore, autoimmune linkages between...
The pancreatic islets of Langerhans, which are small 3D collections specialized endocrine and supporting cells interspersed throughout the pancreas, have a central role in control glucose homeostasis through secretion insulin by beta cells, lowers blood glucose, glucagon alpha raises glucose. Intracellular signaling pathways, including those mediated cAMP, key for regulated cell hormone secretion. islet structure, while essential coordinated function, presents experimental challenges...
The pancreatic islets of Langerhans, which are small 3D collections specialized endocrine and supporting cells interspersed throughout the pancreas, have a central role in control glucose homeostasis through secretion insulin by beta cells, lowers blood glucose, glucagon alpha raises glucose. Intracellular signaling pathways, including those mediated cAMP, key for regulated cell hormone secretion. islet structure, while essential coordinated function, presents experimental challenges...
T2D is characterized by progressive pancreatic islet dysfunction; however, dissecting causes of this dysfunction in humans challenging due to disease heterogeneity and the difficulty obtaining relevant samples. To address these challenges, we used an integrated approach functionally molecularly study native pancreas isolated islets from same human cadaveric donor. Using associated donor clinical information, focused on donors with short duration (n=10, age 47-66 years, 2-7-year duration,...
Impaired counterregulatory response of glucagon to hypoglycemia is a common complication type 1 diabetes (T1D). One proposed contributor abnormal secretion decreased sympathetic innervation in T1D islets. To systematically assess the human pancreatic tissues, we examined samples from donors with recent-onset (<10 years, age 13-45 n = 4), longstanding (>10 27-63 3), and nondiabetic controls (age 10-52 4). Islet exocrine tissue was visualized by pan-neuronal marker tubulin β-3...
ABSTRACT Endogenous β cell regeneration could alleviate diabetes, but proliferative stimuli within the islet microenvironment are incompletely understood. We previously found that recovery following hypervascularization-induced loss involves interactions with endothelial cells (ECs) and macrophages (MΦs). Here we show ECs modulate MΦ infiltration phenotype during loss, recruited MΦs essential for recovery. Furthermore, VEGFR2 inactivation in quiescent accelerates vascular regression leads to...