A5061680106- Adenosine and Purinergic Signaling
- Neuroscience and Neuropharmacology Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Genetic Neurodegenerative Diseases
- Mitochondrial Function and Pathology
- Receptor Mechanisms and Signaling
- Autoimmune Neurological Disorders and Treatments
- Nerve injury and regeneration
- RNA modifications and cancer
- Nicotinic Acetylcholine Receptors Study
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Cancer Cells and Metastasis
- Stress Responses and Cortisol
- Neurological Complications and Syndromes
- Photoreceptor and optogenetics research
- MicroRNA in disease regulation
- Neuroendocrine regulation and behavior
- Tryptophan and brain disorders
- Neurogenesis and neuroplasticity mechanisms
- Critical Theory and Philosophy
- S100 Proteins and Annexins
- Nanoparticle-Based Drug Delivery
- Geological and Geophysical Studies Worldwide
- DNA and Nucleic Acid Chemistry
University of Coimbra
2014-2023
UCLouvain
2013
Universidade Federal de Mato Grosso do Sul
2009
Abstract Synaptic plasticity in the autoassociative network of recurrent connections among hippocampal CA3 pyramidal cells is thought to enable storage episodic memory. Impaired memory an early manifestation cognitive deficits Alzheimer’s disease (AD). In APP/PS1 mouse model AD amyloidosis, we show that associative long-term synaptic potentiation (LTP) abolished at stage. This caused by activation upregulated neuronal adenosine A 2A receptors (A R) rather than dysregulation NMDAR signalling...
Brain-derived neurotrophic factor (BDNF) has been shown to control microglial responses in neuropathic pain. Since adenosine A2A receptors (A2ARs) neuroinflammation, as well the production and function of BDNF, we tested see if A2AR controls microglia-dependent secretion BDNF proliferation cells, a crucial event neuroinflammation.Murine N9 cells were challenged with lipopolysaccharide (LPS, 100 ng/mL) absence or presence antagonist, SCH58261 (50 nM), other modulators signaling. The cellular...
Adenosine A2A receptors (A2AR) overfunction causes synaptic and memory dysfunction in early Alzheimer's disease (AD). In a β-amyloid (Aβ1-42)-based model of AD, we now unraveled that this involves an increased release ATP coupled to density activity ecto-5′-nucleotidase (CD73)-mediated formation adenosine selectively activating A2AR. Thus, CD73 inhibition with α,β-methylene-ADP impaired long-term potentiation (LTP) mouse hippocampal slices, which is occluded upon previous superfusion the...
Objective Machado–Joseph disease (MJD) is a neurodegenerative disorder associated with an abnormal CAG expansion, which translates into expanded polyglutamine tract within ataxin‐3. There no therapy to prevent or modify progression. Because caffeine (a nonselective adenosine receptor antagonist) and selective A 2A (A R) blockade alleviate neurodegeneration in different brain diseases, namely at early stages of another polyglutamine‐related such as Huntington's disease, we now tested their...
Abstract Neurodegeneration is a process transversal to neuropsychiatric diseases and the understanding of its mechanisms should allow devising strategies prevent this irreversible step in brain diseases. caused by seizures critical aggravation temporal lobe epilepsy, but remain undetermined. Convulsions trigger an elevation extracellular adenosine upregulate A 2A receptors (A R), which have been associated with control neurodegenerative Using rat mouse kainate model we now tested whether R...
Machado–Joseph Disease (MJD) is the most prevalent autosomal dominantly inherited cerebellar ataxia. It caused by an expanded CAG repeat in ATXN3 gene, which translates into a polyglutamine tract within ataxin-3 protein. Present treatments are symptomatic and do not prevent disease progression. As calpain overactivation has been shown to contribute mutant proteolysis, translocation nucleus, inclusions formation neurodegeneration, we investigated potential role of inhibition as therapeutic...
Machado–Joseph disease (MJD) is a fatal, dominantly inherited neurodegenerative disorder associated with an expanded polyglutamine tract within the ataxin-3 protein, and characterized by progressive impairment of motor coordination, neurodegeneration specific brain regions, including cerebellum striatum. The currently available therapies do not allow modification progression. Neuropeptide Y (NPY) has been shown to exert potent neuroprotective effects multiple pathways MJD mechanisms disease....
Increased ATP release and its extracellular catabolism through CD73 (ecto-5′-nucleotidase) lead to the overactivation of adenosine A2A receptors (A2AR), which occurs in different brain disorders. A2AR blockade blunts mood memory dysfunction caused by repeated stress, but it is unknown if increased coupled CD73-mediated formation responsible for upon stress. This was now investigated adult rats subject stress 14 consecutive days. Frontocortical hippocampal synaptosomes from stressed displayed...
Extracellular ATP is a danger signal to the brain and contributes neurodegeneration in animal models of Alzheimer's disease through its extracellular catabolism by CD73 generate adenosine, bolstering activation adenosine A 2A receptors (A R).Convulsive activity leads increased release, with resulting morphological alterations being eliminated R blockade.However, it not known if upon convulsions there CD73-mediated coupling between release overactivation, causing neurodegeneration.We now show...
The molecular mechanisms underlying circuit re-wiring in the mature brain remains ill-defined. An eloquent example of adult remodelling is hippocampal mossy fiber (MF) sprouting found diseases such as temporal lobe epilepsy. determinants this retrograde remain unclear. This may involve signaling system(s) controlling axon specification/growth during neurodevelopment reactivated epileptogenesis. Since adenosine A2A receptors (A2AR) control formation/outgrowth and synapse stabilization...
Abstract Extracellular ATP can be a danger signal, but its role in striatal circuits afflicted Parkinson’s disease (PD) is unclear and was now investigated. particularly released at high stimulation intensities from purified nerve terminals of mice, which were endowed with different ATP-P2 receptors (P2R), although P2R antagonists did not alter corticostriatal transmission or plasticity. Instead, extracellularly catabolized into adenosine through CD73 to activate A 2A (A R) modulating...
Cortical interneurons born in the subpallium reach cortex through tangential migration, whereas pyramidal cells their final position by radial migration. Purinergic signaling via P2Y1 receptors controls migration of intermediate precursor from ventricular zone to subventricular zone. It was also reported that blockade A2A (A2AR) somatostatin+ interneurons. Here we found A2AR control cortical projection neurons. In A2AR-knockout (KO) mouse embryos or naïve exposed an antagonist, observed...
Objective Machado–Joseph disease (MJD) is a neurodegenerative spinocerebellar ataxia (SCA) associated with an expanded polyglutamine tract within ataxin‐3 for which there currently no available therapy. We previously showed that caffeine, nonselective adenosine receptor antagonist, delays the appearance of striatal damage resulting from expression full‐length mutant ataxin‐3. Here we investigated ability caffeine to alleviate behavioral deficits and cerebellar neuropathology in transgenic...
Neospora caninum, an Apicomplexan parasite that can causes abortion, is responsible for considerable economic and reproductive losses in livestock.The purpose of the present study was to determine whether recombinant NcSRS2 a suitable indirect ELISA antigen determining specific immune response N. caninum sheep.A total 441 serum samples were subjected IFAT rNcSRS2 based-ELISA, with both tests performing similarly.The sensitivity specificity 98.6 98.3%, respectively.The kappa index shows 0.98...
Adenosine A2A receptors (A2AR) were recently described to control synaptic plasticity and network activity in the prefrontal cortex (PFC). We now probed role of these PFC A2AR by evaluating behavioral performance (locomotor activity, anxiety-related behavior, cost-benefit decision making working memory) rats upon viral shA2AR-mediated downregulation selectively prelimbic medial (PLmPFC). The most evident alteration observed shA2AR-treated rats, when compared sh-control-treated was a decrease...
Notwithstanding the advances in treatment of lung cancer with immune checkpoint inhibitors, high percentage non-responders supports development novel anticancer treatments. Herein, expression onco-target nucleolin patient-derived pulmonary carcinomas was characterized, along assessment its potential as a therapeutic target. The clinical prognostic value for human evaluated through data mining from Cancer Genome Atlas project and immunohistochemical detection samples. Cell surface by flow...
Targeting multiple cellular populations is of high therapeutic relevance for the tackling solid tumors heterogeneity. Herein, ability pegylated and pH-sensitive liposomes, functionalized with nucleolin-binding F3 peptide containing doxorubicin (DXR)/C6-ceramide synergistic combination, to target, in vitro, ovarian cancer, including cancer stem cells (CSC), was assessed. The underlying molecular mechanism action nucleolin-mediated intracellular delivery C6-ceramide also explored. assessment...