A5026424777- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Extracellular vesicles in disease
- Cancer Genomics and Diagnostics
- Microtubule and mitosis dynamics
- MicroRNA in disease regulation
- Colorectal Cancer Screening and Detection
- Nuts composition and effects
- Ferroptosis and cancer prognosis
- RNA Research and Splicing
- Cancer-related Molecular Pathways
- Genomics, phytochemicals, and oxidative stress
- Transplantation: Methods and Outcomes
- Genetic factors in colorectal cancer
- Endoplasmic Reticulum Stress and Disease
- Cancer Cells and Metastasis
Wenzhou Medical University
2021-2024
First Affiliated Hospital of Wenzhou Medical University
2021-2024
Multiplex assays, involving the simultaneous use of multiple circulating tumor DNA (ctDNA) markers, can improve performance liquid biopsies so that they are highly predictive cancer recurrence. We have developed a single-tube methylation-specific quantitative PCR assay (mqMSP) uses 10 different methylation markers and is capable analysis plasma samples with as little 0.05% DNA. In cohort 179 from colorectal (CRC) patients, adenoma healthy controls, sensitivity specificity mqMSP were 84.9%...
Extrachromosomal circular DNAs (eccDNAs) increase the number of proto-oncogenes by enhancing oncogene expression to promote tumorigenesis. However, there are limited reports on differential eccDNA and analysis in lung cancer, especially adenocarcinoma (LAD).Three LAD three corresponding NT tissues samples were used for next-generation sequencing analysis, an additional 20 quantitative PCR (qPCR) evaluations. We further performed qPCR amplification using serum from patients healthy medical...
Lung adenocarcinoma (LAD) is a common malignancy; however, its underlying molecular mechanism unclear.Circular RNAs (circRNAs) serve as significant cancer regulators.The overexpression of circRAPGEF5 in LAD tissues and cells indicated that it may be involved promoting progression.Analysis 61 revealed was related to lymph node metastasis.Functionally, promoted the proliferation, migration, invasion, epithelial-mesenchymal transition vitro growth vivo.Mechanistically, dual-luciferase reporter...
Abstract Copy number variations (CNVs) play a vital role in regulating genes expression and tumorigenesis. We explored the copy alterations early-stage lung adenocarcinoma using high-throughput sequencing nucleic acid flight mass spectrometry technology, found that 8q22.1-22.2 is frequently amplified tissues. COX6C localizes on region its notably enhanced driven by amplification adenocarcinoma. Knockdown of significantly inhibits cell proliferation, induces S-G2/M cycle arrest, mitosis...
Abstract Aim This study aimed to explore the mechanism of LncRNA urothelial carcinoma-associated 1 (UCA1) promoting cisplatin resistance in lung adenocarcinoma (LUAD). Method The UCA1 expression level LUAD cell lines was detected by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). We overexpressed A549 cells and downregulated A549/DDP lentivirus‑mediated technique. Subsequently, vitro, vivo functional experiments were performed investigate roles growth metastasis...
Small GTPases regulate multiple important cellular behaviors and their activities are strictly controlled by a mass of regulators. The dysfunction or abnormal expression small regulators was frequently observed in various cancers. Here, we analyzed the prognostic correlation several related based on TCGA database found that Ankyrin Repeat PH Domain 1 (ARAP1), GTPase activating protein (GAP), is reduced lung adenocarcinoma tissues compared to normal displays positive with overall survival...
Abstract Background Lung adenocarcinoma (LUAD) is a highly malignant tumor with very low five‐year survival rate. In this study, we aimed to identify differentially expressed long‐chain non‐coding RNA (lncRNAs) and mRNAs from benign pleural effusion exosomes. Methods We used gene microassay quantitative real‐time reverse transcription polymerase chain reaction (RT‐qPCR) detect verify lncRNAs in Gene Ontology (GO) functional significance Kyoto Encyclopedia of Genes Genomes (KEGG) pathway...
Abstract Background: The main objective of the current research was to explore mechanism LncRNA UCA1 promoting cisplatin resistance in lung adenocarcinoma (LUAD). Method: expression level LUAD cell lines herein detected by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). We overexpressed A549 and downregulated A549/DDP lentivirus‑mediated technique. analyzed their biological differences function experiments, RNA pulldown, protein mass spectrometry (MS),...
Abstract Purpose: Existing biomarkers for diagnosing and predicting the metastasis of lung adenocarcinoma (LUAD) may not meet demands clinical practice. Risk prediction models based on multiple markers provide better prognostic factors accurate diagnosis metastatic LUAD. Methods: An animal model LUAD was constructed using CRISPR library technology, genes related to were screened by mRNA sequencing normal tissues. The immune characteristics different subtypes analyzed, differential subjected...