A5100403496- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Inflammatory Biomarkers in Disease Prognosis
- Renal cell carcinoma treatment
- Peptidase Inhibition and Analysis
- Sarcoma Diagnosis and Treatment
- Bladder and Urothelial Cancer Treatments
- Renal and related cancers
- Pleural and Pulmonary Diseases
- Vascular Tumors and Angiosarcomas
- Genomics and Chromatin Dynamics
- Lung Cancer Diagnosis and Treatment
- Lymphoma Diagnosis and Treatment
- Cancer-related molecular mechanisms research
- Lung Cancer Treatments and Mutations
- Lung Cancer Research Studies
- Gastric Cancer Management and Outcomes
- 14-3-3 protein interactions
- Pancreatic and Hepatic Oncology Research
- S100 Proteins and Annexins
- Signaling Pathways in Disease
- Esophageal Cancer Research and Treatment
- RNA regulation and disease
- Cancer Genomics and Diagnostics
Peking University
2019-2025
Peking University Third Hospital
2019-2025
Eunice Kennedy Shriver National Institute of Child Health and Human Development
2001-2024
National Institutes of Health
2001-2024
Shandong University
2014-2024
Peking University Shenzhen Hospital
2023-2024
Peking University First Hospital
2024
Sun Yat-sen University
2019-2024
Fifth Affiliated Hospital of Sun Yat-sen University
2019-2024
Beijing Haidian Hospital
2024
Under cell stress, global protein synthesis is inhibited to preserve energy. One mechanism sequester and silence mRNAs in ribonucleoprotein complexes known as stress granules (SGs), which contain translationally silent mRNAs, preinitiation factors, RNA-binding proteins. Y-box binding 1 (YB-1) localizes SGs, but its role SG biology unknown. We now report that YB-1 directly binds activates the 5′ untranslated region (UTR) of G3BP1 thereby controlling availability nucleator for assembly....
Translation initiation factor 2 (eIF2) is a heterotrimeric protein that transfers methionyl-initiator tRNAMet to the small ribosomal subunit in ternary complex with GTP. The eIF2 phosphorylated on serine 51 of its α [eIF2(αP)] acts as competitive inhibitor guanine nucleotide exchange factor, eIF2B, impairing formation and thereby inhibiting translation initiation. eIF2B comprised catalytic regulatory subcomplexes harboring independent binding sites; however, it was unknown whether directly...
The aim of this study is to search the most powerful prognostic factor from routine blood test for esophageal squamous cell cancer (ESCC) patients. Multiple laboratory tests were evaluated including those reflecting red parameters (hemoglobin (Hb), mean corpuscular volume (MCV), hemoglobin concentration (MCHC), and distribution width (RDW)), platelet morphological (mean (MPV) count (PLT)), coagulation status (D-dimer), tumor biomarker (CA19-9). Known inflammatory indices (NLR PLR) also...
Although gemcitabine is the standard chemotherapy treatment for advanced pancreatic cancer, its benefits are quite limited due to prevalent chemoresistance, and mechanism underlying chemoresistance remains unclear. Currently, Nrf2 has been deemed as a significant contributor in cancer. Brusatol unique inhibitor of pathway, previous studies, we determined that brusatol exhibits effects growth inhibition proapoptosis cancer cells. Due these data, speculate can reverse gemcitabine‐induced...
Background The systemic immune‐inflammation index (SII) is correlated with patient survival in various types of solid tumors. However, only a few studies have focused on the prognostic value SII patients surgically resected non‐small cell lung cancer (NSCLC). Methods This study was single center retrospective analysis 569 NSCLC who underwent curative lobectomy at Department Thoracic Surgery, National Cancer Center/Cancer Hospital, Chinese Academy Medical Sciences and Peking Union College...
Abstract Numerous cancers, including prostate cancer (PCa), are addicted to transcription programs driven by specific genomic regions known as super-enhancers (SEs). The robust of genes at such SEs is enabled the formation phase-separated condensates factors and coactivators with intrinsically disordered regions. androgen receptor (AR), main oncogenic driver in PCa, contains large co-recruited transcriptional coactivator mediator complex subunit 1 (MED1) androgen-dependent PCa cells, thereby...
Background:The systemic immune-inflammation index (SII) has been reported to be associated with patient survival in various kinds of solid tumors.However, just few studies have focused on its prognostic value patients surgically resected esophageal squamous cell carcinoma (ESCC). Materials and Methods:This study was a single-institution, retrospective analysis 468 ESCC who underwent curative esophagectomy at the Department Thoracic Surgery, National Cancer Center/Cancer Hospital, Chinese...
Cells limit energy-consuming mRNA translation during stress to maintain metabolic homeostasis. Sequestration of mRNAs by RNA binding proteins (RBPs) into granules reduces their translation, but it remains unclear whether RBPs also function in partitioning specific transcripts polysomes (PSs) guide selective and adaptation cancer. To study transcript under cell stress, we catalogued enriched prostate carcinoma PC-3 PSs, as defined polysome fractionation sequencing (RNAseq), compared them...
The positive transcription elongation factor b (P-TEFb) contains cyclin T1 (CycT1) and cyclin-dependent kinase 9 (Cdk9). For activating the expression of eukaryotic genes, histidine-rich sequence in CycT1 binds heptapeptide repeats C-terminal domain (CTD) RNA polymerase II (RNAPII), whereupon Cdk9 phosphorylates CTD. We found that alanine-substituted cannot be phosphorylated also bind CycT1. When placed near units, these CTD analogs block effects P-TEFb. Remarkably, transcriptional repressor...
Mediator is a multisubunit coactivator required for initiation by RNA polymerase II. The tail subdomain, containing Med15/Gal11, target of the activator Gcn4 in vivo, critical recruitment native or subdomain present sin4Delta cells. Although several Gal11 segments were previously shown to bind vitro, importance these interactions and transcriptional activation cells was unknown. We show that interaction with vitro this subcomplex intact ARG1 promoter vivo involve additive contributions from...
Abstract Background Eukaryotic translation initiation factor 4G (eIF4G) is thought to influence the translational efficiencies of cellular mRNAs by its roles in forming an eIF4F-mRNA-PABP mRNP that competent for attachment 43S preinitiation complex, and scanning through structured 5' UTR sequences. We have tested this hypothesis determining effects genetically depleting eIF4G from yeast cells on global (TEs), using gene expression microarrays measure abundance mRNA polysomes relative total...
We compared outcomes in patients treated with radio frequency ablation or partial nephrectomy for clinical cT1b renal cell carcinoma.We retrospectively reviewed the records of all who underwent between February 2006 and December 2010. Radiographic followup contrast imaging was performed 7 days, 3 6 months, every months thereafter after sequentially. The protocol initial years annually thereafter. Kaplan-Meier method used to generate survival curves, which were log rank test. Multivariable...
To compare outcomes in patients treated with radiofrequency ablation (RFA) and partial nephrectomy (PN) for clinical T1a renal-cell carcinoma (RCC) a propensity-score matched cohort.We conducted retrospective review of the records all who underwent RFA or between February 2005 December 2009 our institution. The Kaplan-Meier method was used to generate survival curves that were compared log-rank test. Univariable multivariable regression analyses performed determine predictors survival.A...
Summary Background Previous studies have demonstrated the efficacy of apatinib and anlotinib for treatment sarcomas. However, more clinical data evidence are needed to support selection study design. Here, we evaluated effectiveness safety these two drugs Methods We retrospectively reviewed 110 patients with advanced osteosarcoma ( n = 32) or soft tissue sarcoma (STS, 78) who received oral therapy during May 2016–February 2019 at centers. Patients were divided into groups. Results Among...
Acute lung injury (ALI) is a critical event involved in the pathophysiological process of acute pancreatitis (AP). Many methods have been widely used for treatment AP-ALI, but few are useful during early inflammation. Lipoxin A4 (LXA4), potent available anti-inflammatory and novel antioxidant mediator, has extensively studied its underlying mechanism as protective mediator not clear. This research was conducted to identify possible targets mechanisms anti-AP-ALI effect LXA4. First, we...
Neutral-to-lymphocyte ratio (NLR), lymphocyte-to-monocyte (LMR), and platelet-to-lymphocyte (PLR) are associated with coronavirus disease 2019 (COVID-19) many diseases, but there few data about the reference interval (RI) of NLR, LMR, PLR.The neutrophil count, lymphocyte monocyte platelet count 404,272 Chinese healthy adults (>18 years old) were measured by Sysmex XE-2100 automatic hematology analyzer, PLR calculated. According to CLSI C28-A3, nonparametric 95% percentile is defined as...
Protein kinase Gcn2 attenuates protein synthesis in response to amino acid starvation while stimulating translation of a transcriptional activator biosynthesis. activation requires domain related histidyl-tRNA synthetase (HisRS), the enzyme that aminoacylates tRNA
Lymphatic leakage is a common complication after radical cystectomy and pelvic lymph node dissection (PLND) for muscle-invasive bladder cancer (MIBC).This study aimed to investigate the risk factors contributing postoperative lymphatic in patients with MIBC. A total of 534 undergoing PLND were enrolled retrospective at Peking University Third Hospital from January 2010 July 2023. Patients categorized into leakage(n = 254)and non-leakage groups (n 280) compared demographic, perioperativ...
<h3>Importance</h3> Randomized clinical trials demonstrate no benefit for epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors in unselected patients with head and neck squamous cell carcinoma (HNSCC). However, a patient stage IVA HNSCC received 13 days of neoadjuvant erlotinib experienced near-complete histologic response. <h3>Objective</h3> To determine mechanism exceptional response to therapy HNSCC. <h3>Design, Setting, Participants</h3> Single locally advanced who...
Findings remain unclear whether neutrophil-to-lymphocyte ratio (NLR) detrimentally affects advanced nasopharyngeal carcinoma (NPC) prognosis. We aim to evaluate the prognostic value of NLR in patients with NPC based on a large-scale cohort from an endemic area.We selected retrospectively examining long-term cancer outcomes following diagnosis. Neutrophil counts and lymphocyte were assessed prior treatment. Kaplan-Meier method log-rank test used calculate compare survival outcomes....
Scd6 protein family members are evolutionarily conserved components of translationally silent mRNA granules. Yeast interacts with Dcp2 and Dhh1, respectively a subunit regulator the decapping enzyme, also associates translation initiation factor eIF4G to inhibit in cell extracts. However, role turnover translational repression vivo is unclear. We demonstrate that tethering GFP reporter reduces abundance via suppresses Dhh1. Thus, dcp2Δ mutant, tethered expression little effect on abundance,...
Abstract Background Tyrosine kinase inhibitors (TKIs) such as cabozantinib, regorafenib have demonstrated encouraging activity in prolonging progression‐free survival (PFS) several bone sarcoma entities prospective clinical trials. This retrospective study aims to analyze the safety and efficacy of anlotinib, a novel multi‐target TKI, patients with locally unresectable or metastatic at three institutions. Methods Patients advanced administered anlotinib 12 mg once daily, 2 weeks on/1 week...