A5075608227- Cholesterol and Lipid Metabolism
- Estrogen and related hormone effects
- Cancer, Lipids, and Metabolism
- Dermatology and Skin Diseases
- biodegradable polymer synthesis and properties
- Microplastics and Plastic Pollution
- Skin and Cellular Biology Research
- Recycling and Waste Management Techniques
- Polymer Surface Interaction Studies
- Advanced Polymer Synthesis and Characterization
- Advancements in Transdermal Drug Delivery
- Electrospun Nanofibers in Biomedical Applications
- Tissue Engineering and Regenerative Medicine
- Ocular Surface and Contact Lens
- Dendrimers and Hyperbranched Polymers
- Allergic Rhinitis and Sensitization
- Nanoplatforms for cancer theranostics
- Steroid Chemistry and Biochemistry
- Photodynamic Therapy Research Studies
- Drug Transport and Resistance Mechanisms
- Nanomaterials for catalytic reactions
- Contact Dermatitis and Allergies
- Cellular transport and secretion
- Free Radicals and Antioxidants
- Phagocytosis and Immune Regulation
Université Toulouse III - Paul Sabatier
2008-2024
Université de Toulouse
2014-2023
Hôpital Rangueil
2016-2019
Chongqing Electromechanical Holdings (China)
2014-2015
Institut des Maladies Métaboliques et Cardiovasculaires
2014
Institut de Mathématiques de Toulouse
2014
Laboratoire d'Énergétique Moléculaire et Macroscopique, Combustion
2001-2006
Laboratoire de Biochimie
2001-2003
Inserm
2002-2003
Centre de Recherche des Cordeliers
2002
The global estimation of microplastic afloat in the ocean is only approximately 1% annual plastic inputs. This reflects fundamental knowledge gaps transformation, fragmentation, and fates microplastics ocean. In order to better understand fragmentation we proceeded a thorough physicochemical characterization samples collected from North Artlantic subtropical gyre during sea campaign Expedition seventh Continent May 2014. results were confronted with mathematical approach. introduction mass...
The ability to self-assemble was evaluated for a large variety of amphiphilic block copolymers, including poly(ethyleneoxide-b-ε-caprolactone), poly(ethyleneoxide-b-d,l-lactide), poly(ethyleneoxide-b-styrene), poly(ethyleneoxide-b-butadiene) and poly(ethyleneoxide-b-methylmethacrylate). Different methods formation are discussed, such as cosolvent addition, film hydration or electroformation. influence experimental parameters macromolecular structures on the size morphology final...
Plastic pollution has become a worldwide concern. It was demonstrated that plastic breaks down to nanoscale particles in the environment, forming so-called nanoplastics. is important understand their ecological impact, but structure not elucidated. In this original work, we characterize microstructure of oceanic polyethylene debris and compare it nonweathered objects. Cross sections are analyzed by several emergent mapping techniques. We highlight deep modifications within layer few hundred...
Abstract The microsomal antiestrogen-binding site (AEBS) is a high-affinity membranous binding for the antitumor drug tamoxifen that selectively binds diphenylmethane derivatives of such as PBPE and mediates their antiproliferative properties. AEBS hetero-oligomeric complex consisting 3β-hydroxysterol-Δ8-Δ7-isomerase 3β-hydroxysterol-Δ7-reductase. High-affinity ligands inhibit these enzymes leading to massive intracellular accumulation zymostenol or 7-dehydrocholesterol (DHC), thus linking...
Little is known regarding the molecular mechanisms of atherogenicity triglyceride-rich lipoproteins such as very low-density (VLDLs). We examined effect VLDL on proliferation rat aortic smooth muscle cells, intracellular Ca2+ handling, and activity cAMP-responsive element binding protein (CREB) nuclear factor activated T cells (NFAT) transcription factors. VLDL, isolated from human serum, dose- time-dependently promoted proliferation. After 4 hours exposure to (0.15 g/L proteins),...
Interventional strategies to treat atherosclerosis, such as transluminal angioplasty and stent implantation, often cause vascular injury. This leads intimal hyperplasia (IH) formation that induces inflammatory fibroproliferative processes ultimately restenosis. We show phosphoinositide 3-kinase γ (PI3Kγ) is a key player in IH valid therapeutic target its prevention/treatment. PI3Kγ-deficient mice expressing catalytically inactive PI3Kγ (PI3Kγ KD) showed reduced arterial occlusion...
An amphiphilic polymer (CmPOX) based on poly(2-methyl-2-oxazoline) linked to a hydrophobic part composed of an aliphatic chain ending with photo-active coumarin group has been synthesized. It exhibits the ability forming small polymeric self-assemblies, typically ca. 10 nm in size, which were characterized by TEM, cryo-TEM and DLS. The nanocarriers further formulated yield photo-crosslinked systems dimerization units coumarin-functionalized poly(methyl methacrylate) (CmPMMA) CmPOX. formed...
Atopic dermatitis (AD), the most common inflammatory skin disorder, is a multifactorial disease characterized by genetic predisposition, epidermal barrier disruption, strong T helper (Th) type 2 immune reaction to environmental antigens and an altered cutaneous microbiome. Microbial dysbiosis prevalence of Staphylococcus aureus (S. aureus) has been shown exacerbate AD. In recent years, in vitro models AD have developed, but none them reproduce all pathophysiological features. To better mimic...
Overexpression of human apolipoprotein A-II (hapo A-II) in transgenic mice (hAIItg mice) induced marked hypertriglyceridemia and low levels plasma high density lipoprotein (HDL) with a hapo content. We sought to determine whether cholesterol efflux HDL from these would be affected. In the Fu5AH cell system, hAIItg markedly lower than did control plasma, accordance dependence on concentration. Moreover, HDLs were less effective acceptors HDLs. J774 macrophage pretreatment cAMP, which...
The architecture of PNIPAM based polymers strongly influences both their thermoresponsiveness and abilities to control stabilize AuNP formation.
Abstract Background Fu5AH rat hepatoma cells and cAMP (cyclic AMP)‐pretreated J774 mouse macrophages are commonly used as models for SR‐BI (scavenger receptor class B type I) ABCA1 (ATP binding cassette transporter 1)‐mediated free cholesterol efflux to whole serum, respectively. However, the responsiveness of Fu5AH, control or pretreated various lipids HDL (high‐density lipoprotein)‐parameters from both normo‐ dyslipidaemic subjects has never been compared within same study. Materials...
Mesomorphic hyperbranched polymers comprising ionic functionalities interacting with inorganic precursors allow the formation of controlled-size nanoparticles.
Human Platelet lysate, a hemoderivative product rich in growth factors, holds significant promise tissue engineering and regenerative medicine. However, its practical utility liquid or gel form is hampered by challenges such as limited stability handling difficulties. This study aimed to engineer dry porous aerogels from platelet lysate hydrogel using an environmen-tally friendly supercritical CO2-based shaping process customized for appli-cations. The resulting demonstrated remarkable...
Absence of a functional proteasome in the suprabasal layers epidermis is responsible for keratosis linearis with ichthyosis congenital and sclerosing keratoderma syndrome. Patient shows hypergranulosis associated abnormally shaped keratohyalin granules abnormal distribution filaggrin Stratum granulosum corneum. This suggests that involved degradation filaggrin. To test this hypothesis, proteolytic activity was inhibited 3D reconstructed human (RHE) specific clasto-lactacystin β-lactone...
Deimination is a post-translational modification catalyzed by family of enzymes named peptidylarginine deiminases (PADs). PADs transform arginine residues protein substrates into citrulline. has been associated with numerous physiological and pathological processes. In human skin, three are expressed (PAD1-3). While PAD3 important for hair shape formation, the role PAD1 less clear. To decipher main role(s) in epidermal differentiation, its expression was down-regulated using...
Abstract Congenital anomalies of the kidney and urinary tract (CAKUT) are first cause chronic disease in childhood. Several genetic environmental origins associated with CAKUT, but most pathogenic pathways remain elusive. Considering amniotic fluid (AF) composition as a proxy for fetal development, we analyzed AF proteome from non‐severe CAKUT ( n = 19), severe 14), healthy control 22) fetuses using LC–MS/MS. We identified 471 significant proteins that discriminated three groups 81%...
In the nanomedicine field, there is a need to widen availability of nanovectors compensate for increasingly reported side effects poly(ethene glycol). Nanovectors enabling cross-linking can further optimize drug delivery. Cross-linkable polyoxazolines are therefore relevant candidates address these two points. Here we present synthesis coumarin-functionalized poly(2-alkyl-2-oxazoline) block copolymers, namely, poly(2-methyl-2-oxazoline)-block-poly(2-phenyl-2-oxazoline) and...