A5083402775- Adipose Tissue and Metabolism
- Muscle Physiology and Disorders
- Muscle metabolism and nutrition
- Exercise and Physiological Responses
- Diet and metabolism studies
- Angiogenesis and VEGF in Cancer
- Cancer, Hypoxia, and Metabolism
- Diet, Metabolism, and Disease
- Genetic Neurodegenerative Diseases
- Mitochondrial Function and Pathology
- Nutrition and Health in Aging
- Sirtuins and Resveratrol in Medicine
- Adipokines, Inflammation, and Metabolic Diseases
- RNA modifications and cancer
- Immune Cell Function and Interaction
- Dietary Effects on Health
- Synthesis and Catalytic Reactions
- Phytochemicals and Antioxidant Activities
- Ubiquitin and proteasome pathways
- Birth, Development, and Health
- Histone Deacetylase Inhibitors Research
- Plant-based Medicinal Research
- RNA Research and Splicing
- Synthesis of β-Lactam Compounds
- Circadian rhythm and melatonin
Nihon University
2024
National Sanyo Hospital
2024
National Institute of Advanced Industrial Science and Technology
2019-2023
Hakodate National Hospital
2019
Tokushima University
1992-2017
Institute of Biomedical Science
2015
Bio-Medical Science (South Korea)
2015
Hokkaido University
2010
Yokohama National University
2008
Tohoku University
1994-2006
Ketogenic diets (KD) that are very high in fat and low carbohydrates thought to simulate the metabolic effects of starvation. We fed mice with a KD for seven days assess underlying mechanisms muscle wasting induced by chronic This diet decreased weight gastrocnemius (Ga), tibialis anterior (TA) soleus (Sol) muscles 23%, 11% 16%, respectively. The size Ga, TA, Sol fibers grip strength four limbs also significantly declined 20%, 28%, 16% 22%, atrophy-related genes Mafbx, Murf1, Foxo3, Lc3b...
Little is known about the precise physiological roles of uncoupling protein 1 (UCP1) homologs (UCP2, UCP3, avian UCP) whose levels are up‐regulated during fasting. UCPs in skeletal muscle thought to play a role regulation lipids as fuel substrates, and/or controlling production reactive oxygen species (ROS). The aim this investigation, using from fasted chickens, was examine alterations expression genes encoding for UCP and key enzymes relevant lipid flux across mitochondrial β‐oxidation...
Proinflammatory cytokines are factors that induce ubiquitin-proteasome-dependent proteolysis in skeletal muscle, causing muscle atrophy. Although isoflavones, as potent antioxidative nutrients, have been known to reduce damage during the catabolic state, non-antioxidant effects of isoflavones against atrophy not well known. Here we report on inhibitory such genistein and daidzein caused by tumor necrosis factor (TNF)-α treatment. In C2C12 myotubes, TNF-α treatment markedly elevated...
Muscle atrophy is a complex process that occurs as consequence of various stress events. atrophy-associated genes (atrogenes) such atrogin-1/MAFbx and MuRF-1 are induced early in the process, increase their expression precedes loss muscle weight. Although antioxidative nutrients suppress atrogene skeletal cells, inhibitory effects flavonoids on inflammation-induced have not been clarified. Here, we investigated lipopolysaccharide (LPS)-induced expression. We examined whether nine belonging...
Oxidative stress is a key factor in stimulating the expression of atrogenes, which are muscle atrophy-related ubiquitin ligases, skeletal muscle, and it induces atrophy during unloading. However, effects antioxidative nutrients on atrogene have not been demonstrated. We report inhibitory polyphenols, such as epicatechin (EC), gallate (ECg) epigallocatechin (EGCg) quercetin, up-regulated by three dimensional (3D)-clinorotation or glucocorticoid. These treatments markedly elevated including...
Polymethoxyflavones are flavonoids that abundant in citrus fruit peels and have beneficial effects on human health. Previous studies demonstrated the polymethoxyflavones, namely sudachitin nobiletin, ameliorate obesity diabetes humans rodents. Although nobiletin induces lipolysis adipocytes, lipolytic pathway activation by has not been clarified adipocytes. In this study, effect of was elucidated murine 3T3-L1 Glycerol release into medium cyclic AMP (cAMP)/protein kinase A...
Skeletal muscle is one of the most sensitive tissues to mechanical loading, and unloading inhibits regeneration potential skeletal after injury. This study was designed elucidate specific effects stress on function immunocytes during We examined immunocyte infiltration in cardiotoxin (CTX)-injected soleus muscles tail-suspended (TS) mice. In CTX-injected TS mice, cross-sectional area regenerating myofibers smaller than that weight-bearing (WB) indicating delays following CTX-induced damage....
We previously reported the potential involvement of casitas B-cell lymphoma-b (Cbl-b) in aging-related murine insulin resistance. Because obesity also induces macrophage recruitment into adipose tissue, we elucidated here role Cbl-b obesity-related Cbl-b(+/+) and Cbl-b(-/-) mice were fed a high-fat diet (HFD) then examined for changes signaling. The HFD caused macrophages tissue increased inflammatory reaction compared with mice. Peritoneal from Cbl-b-overexpressing RAW264.7 used to examine...
Background: In recent years, the number of bedridden people is rapidly increasing due to aging or lack exercise in Japan. This problem becoming more serious, since there no countermeasure against it. present study, we designed investigate whether dietary proteins, especially soy, had beneficial effects on skeletal muscle 59 volunteers with various physical activities. Methods: We subjected activities meal intervention examination. Persons low and high were divided into two groups, casein...
Background. Unloading stress induces skeletal muscle atrophy. We have reported that Cbl-b ubiquitin ligase is a master regulator of unloading-associated The present study was designed to elucidate whether dietary soy glycinin protein prevents denervation-mediated atrophy, based on the presence inhibitory peptides against in protein. Methods. Mice were fed either 20% casein diet, isolate 10% diet containing casein, or diet. One week later, right sciatic nerve cut. wet weight, cross sectional...
Feminized 2-year-old eels were reared in freshwater at 26℃ until October, then the water temperature was gradually decreased to 16℃ December, and increased from February April. They received weekly injections of salmon pituitary extract (body weight, 15 or 30 mg/kg) induce sexual maturation September, The quality eggs obtained each season evaluated by resulting fertilization, hatching, 8-day sur- vival rates. High via high-dose most experimental groups. In injection groups, induced final...
Abstract Deficiency of the Cbl‐b ubiquitin ligase gene activates macrophages in mice. This study aimed to elucidate pathophysiological roles muscle degeneration/regeneration ‐deficient We examined immune cell infiltration and cytokine expression cardiotoxin‐injected tibialis anterior Ablation delayed regeneration cardiotoxin‐induced skeletal damage compared with wild‐type CD8‐positive T cells were still present damaged on day 14 after cardiotoxin injection mice, but there was dispersal same...
A number of anti-angiogenic drugs targeting vascular endothelial growth factor receptors (VEGF-R) have developed and enabled significant advances in cancer therapy including colorectal cancer. However, acquired resistance to the occurs, leading disease progression, such as invasion metastasis. How tumors become promote their malignancy remains fully uncertain. One possible mechanisms for progression may be direct effect VEGF-R inhibitors on tumor cells expressing VEGF-R. We investigated here...
Uncoupling protein 3 (UCP3) and pyruvate dehydrogenase kinase 4 (PDK4) in skeletal muscle are key regulators of the glucose lipid metabolic processes that involved insulin resistance. Medium-chain fatty acids (MCFAs) have anti-obesogenic effects rodents humans, while long-chain (LCFAs) cause increases body weight To clarify beneficial MCFAs, we examined UCP3 PDK4 expression muscles mice fed a MCFA- or LCFA-enriched high-fat diet (HFD). Five-week feeding HFD caused high gain induced...
Processed aconite root (PA), the tuberous of Aconitum carmichaelii prepared by autoclaving, is a crude drug used in Japanese traditional Kampo medicine and Chinese for symptoms kidney deficiency, that related to muscle atrophy modern medicine. The objective present study evaluate effectiveness PA on find its active ingredients using dexamethasone-induced ring finger protein-1 (MuRF1) mRNA expression murine myoblast C2C12 cells. Dexamethasone-induced MuRF1 was significantly suppressed...
Thromboxane (TX) is a vasoactive hormone which known to be involved in renal physiology and pathophysiology. To identify the site of action TX kidney, we examined distribution this receptor normal rat kidney using polyclonal antibody against had raised. Western immunoblot membrane fractions with identified single protein band at predicted molecular mass protein. In immunostainable was observed glomeruli, arterial walls, luminal membranes thick ascending limbs Henle's loop, basolateral either...
There is evidence that several messenger RNAs (mRNAs) are bifunctional RNAs, i.e. RNA transcript carrying both protein-coding capacity and activity as functional non-coding via 5′ 3′ untranslated regions (UTRs). In this study, we identified a novel transcribed from insulin receptor substrate-1 (Irs-1) gene with full-length 5′UTR sequence (FL-Irs-1 mRNA). FL-Irs-1 mRNA was highly expressed only in skeletal muscle tissue. cultured C2C12 cells, the functioned mRNA. The produced IRS-1 protein...
Avian uncoupling protein (avUCP), sharing 71-73% amino acid homology with both UCP2 and UCP3, is one of the mitochondrial anion carrier proteins. Its precise physiological roles in cell remain elusive. A confusing aspect these UCP variants (namely, UCP2, UCP3 avUCP), that their expression enhanced response to fasting ; is, basal metabolic state which such energy expenditure would be expected depressed. In this study, we examined progressive alterations genes encoding for proteins, not only...