A5068193801- Radiopharmaceutical Chemistry and Applications
- Medical Imaging Techniques and Applications
- Medical Imaging and Pathology Studies
- Peptidase Inhibition and Analysis
- Virus-based gene therapy research
- Cancer Research and Treatments
- Chemical Synthesis and Analysis
- Cancer, Hypoxia, and Metabolism
- Neuroblastoma Research and Treatments
- T-cell and B-cell Immunology
- Chemical Synthesis and Characterization
- CAR-T cell therapy research
- Synthesis and Biological Evaluation
- Advanced NMR Techniques and Applications
- Atomic and Subatomic Physics Research
- Heavy Metal Exposure and Toxicity
- Monoclonal and Polyclonal Antibodies Research
- Cancer therapeutics and mechanisms
- Lung Cancer Treatments and Mutations
- Mast cells and histamine
- Trace Elements in Health
- Radiomics and Machine Learning in Medical Imaging
- Lanthanide and Transition Metal Complexes
- Electrochemical Analysis and Applications
St Thomas' Hospital
2010-2023
King's College London
2010-2022
Lambeth Hospital
2021
St. Thomas Hospital
2021
Purpose Accumulation of iodide and other substrates via the human sodium/iodide symporter (hNIS) is fundamental to imaging therapy thyroid disease, hNIS reporter gene hNIS-mediated therapy. There no readily available positron emission tomography (PET) tracer for hNIS. Our aim was develop a colon carcinoma cell line stably expressing hNIS, use it evaluate novel PET tracer, [18F]-tetrafluoroborate. Methods Colon line, HCT116, transfected with thus producing HCT116-C19, high expression. A...
Metastasis is responsible for most cancer deaths. Despite extensive research, the mechanistic understanding of complex processes governing metastasis remains incomplete. In vivo models are paramount but require refinement. Tracking spontaneous by non-invasive in imaging now possible, challenging as it requires long-time observation and high sensitivity. We describe a longitudinal combined radionuclide fluorescence whole-body approach tracking tumor progression metastasis. This reporter gene...
Thallium-201 is a radionuclide that has previously been used clinically for myocardial perfusion scintigraphy. Although in this role it now largely replaced by technetium-99 m radiopharmaceuticals, thallium-201 remains attractive the context of molecular therapy cancer micrometastases or single circulating tumour cells. This due to its Auger electron (AE) emissions, which are amongst highest total energy and number per decay AE-emitters. Currently, chemical platforms achieve potential...
Pretargeting is widely explored in immunoPET as a strategy to reduce radiation exposure of non-target organs and allow the use short-lived radionuclides that would not otherwise be compatible with slow pharmacokinetic profiles antibodies. Here we investigate pretargeting based on gallium-68 chelator THPMe high-affinity pair capable combining vivo. After confirming ability bind 68Ga vivo at low concentrations, bifunctional THPMe-NCS was conjugated humanised huA33 antibody targeting A33...
Nitrogen-13 is an attractive but under-used PET radionuclide for labelling molecules of biological and pharmaceutical interest, complementing other radionuclides. Its short half-life (t1/2 = 9.97 min) imposes synthetic challenges, we have expanded the hitherto limited pool 13N strategies tracers by adapting multicomponent Hantzsch condensation reaction to prepare a library 13N-labelled 1,4-dihydropyridines from [13N]ammonia, including widely-used drug nifedipine. This represents key advance...
Metastasis is responsible for most cancer deaths. Despite extensive research, the mechanistic understanding of complex processes governing metastasis remains incomplete. In vivo models are paramount but require refinement. Tracking spontaneous by non-invasive in imaging now possible, challenging as it requires long-time observation and high sensitivity. We describe a longitudinal combined radionuclide fluorescence whole-body approach tracking tumor progression metastasis. This reporter gene...
Nitrogen-13 has a 10-min half-life which places time constraints on the complexity of viable synthetic methods for its incorporation into PET imaging agents. In exploring ways to overcome this limitation, we have used Ugi reaction develop rapid one-pot method radiolabelling peptidic molecules using [13N]NH3 as precursor.Carrier-added (50 μL) was added solution carboxylic acid, aldehyde, and isocyanide in 2,2,2-TFE (200 μL). The mixture heated microwave synthesiser at 120 °C 10 min. Reactions...
Background: Multi-tracer PET/SPECT imaging enables different modality tracers to be present simultaneously, allowing multiple physiological processes imaged in the same subject, within a short time-frame. Fluorine-18 and technetium-99m, two commonly used PET SPECT radionuclides respectively, possess emission profiles, offering potential for one presence of other. However, impact each radionuclide on scanning other could significant lead confounding results. Here we use combinations 18F 99mTc...
KP46 (tris(hydroxyquinolinato)gallium(III)) is an experimental, orally administered anticancer drug. Its absorption, delivery to tumours, and mode of action are poorly understood. We aimed gain insight into these issues using gallium-67 gallium-68 as radiotracers with SPECT PET imaging in mice. [
IgE antibodies elicit powerful immune responses, recruiting effector cells to tumors more efficiently and with greater cytotoxicity than IgG antibodies. Consequently, are a promising alternative conventional IgG-based therapies in oncology (AllergoOncology). As the pharmacokinetics of less well understood, we used molecular imaging mice compare distribution elimination targeting human tumor-associated antigen chondroitin sulfate proteoglycan 4 (CSPG4).Anti-CSPG4 IgG1 Fc domains were...
Cell labelling agents that enable longitudinal