OBJECTIVE: To test the hypothesis that platelet-rich fibrin glue (PR-FG) can be used clinically as a scaffold to deliver autologous culture-expanded bone marrow mesenchymal stem cells (BM-MSCs) for cartilage repair and report clinical results 1 y after implantation of MSCs PR-FG. PATIENTS AND METHODS: Autologous BM-MSCs were culture expanded, placed on PR-FG intraoperatively, then transplanted into 5 full-thickness defects femoral condyles patients covered with an periosteal flap. Patients...

Abstract Background This work aimed to study the homing evidence and reparative effect of mesenchymal stem cells (MSCs) in healing process induced osteoarthritis experimental animal model (donkeys). Methods Twenty-seven donkeys were equally divided into 3 groups based on observation period after induction arthritis (3, 6 9 weeks) achieve different degrees osteoarthritis. Each group was subdivided three subgroups animals each follow-up (1, 2 months) treatment. The done through intra-articular...

Abstract Background The present study was conducted to evaluate the tumor suppressive effects of bone marrow derived mesenchymal stem cells (MSCs) in an experimental hepatocellular carcinoma (HCC) model rats and investigate possible role Wnt signaling hepato-carcinogenesis. Methods Ninety were included divided equally into: Control group, which received MSCs only, vehicle HCC group induced by diethylnitroseamine (DENA) CCl 4 , after induction, before induction. Histopathological examination...

Stem cell therapy holds a great promise for the repair of injured tissues and organs, including kidney. We studied effect mesenchymal stem cells (MSC) on experimental diabetic nephropathy (DN) in rats possible paracrine signals that mediate their action.Rats were divided into controls, DN rats, receiving MSCs. MSCs given dose (106cells) by intravenous injection. After 4 weeks, 24 h urinary albumin, serum urea creatinine concentrations, transforming growth factor β (TGF β), tumor necrosis α...

Aim . This study aimed to assess seminal miRNA relationship with apoptotic markers and oxidative stress (OS) in infertile men associated varicocele (Vx). Methods In all, 220 subjects were divided into the following groups: fertile normozoospermic men, Vx, oligoasthenoteratozoospermic (OAT) without OAT Vx. They subjected history taking, clinical examination, semen analysis. their semen, estimated: miRNA-122, miRNA-181a, miRNA-34c5 using quantitative real-time PCR, (BAX, BCL2) protein...

The hippocampus is a key brain area for many forms of learning and memory particularly sensitive to changes in glucose homeostasis.To investigate experimentally induced type 1 2 diabetes mellitus rat model the effect on cognitive functions related markers hippocampal synaptic plasticity, possible impact blocking N-methyl-D-aspartic acid (NMDA) receptors by memantine.Seven groups were included: non-diabetic control receiving memantine; type-1 diabetic - untreated, treated with insulin alone...

ObjectiveTo investigate the effect of bridging defects in chronic spinal cord injury using peripheral nerve grafts combined with a chitosan-laminin scaffold and enhancing regeneration through them by co-transplantation bone-marrow-derived mesenchymal stem cells.MethodsIn 14 patients paraplegia caused injury, were grafted cells injected into whole construct contained paste. Patients evaluated International Standards for Classification Spinal Cord Injuries.ResultsChitosan disintegration...

The aim of this study was to investigate the effect a regenerative therapy comprising mesenchymal stem cells (MSCs) pretreated with melatonin (MT) as new for underlying diabetic nephropathy (DN) pathogenesis in rat model, and its possible on autophagy protein Beclin-1. Forty adult male albino Wistar rats were distributed among 4 groups: (i) control, (ii) DN, (iii) MSC-treated, (iv) treated MSCs that pre-incubated in-vitro MT (5 μmol·L –1 24 h; + MT). treatment significantly improved renal...

Background Programmed cell death 1 (PD-1) is a surface protein that serves as an immune checkpoint in conjunction with its two ligands, PD-L1 and PD-L2. Recently, there has been lot of interest the role PD-1/PD-L1 pathway immunoregulation. Objective To assess both PD-1 levels vitiligo patients’ marginal nonlesional biopsies compared normal controls to correlate them disease parameters. Patients methods A total 30 vitiliginous patients age sex-matched were included. Full history clinical...

Abastract ;Preeclampsia (PE) is one of the leading causes prenatal and maternal deaths; therefore developing new biomarkers at earliest stages illness essential to accelerating effectiveness. Using serum microRNA 149 copeptin diagnose preeclampsia will be investigated in this study. We conducted a matched case–control study Egyptian women. Seventy women between 28 40 weeks pregnancy participated divided into two groups: 20 pregnant without PE 50 with PE. Blood biochemical parameters were...

Psoriasis is a disorder with genetic and immunologic background. Leptin can regulate the T-helper response.Our primary goal to study functional polymorphism (G-2548A) of leptin (LEP) gene in predisposition psoriasis, our secondary examine factors affecting plasma levels psoriasis compare patients without metabolic syndrome (MS).The involved 94 100 healthy controls. Analysis G-2548A LEP was made by PCR restriction fragment length technique. The relationship between clinical features analysed....

To investigate the role of paraoxonase 1 (PON1) and vitamin E in pathogenesis some autoimmune diseases, to correlate their levels with disease activity.This randomized case control study was performed on 60 subjects: 45 patients [suffering from psoriasis, vitiligo alopecia areata (AA) 15 each group] healthy controls. Venous blood tissue biopsy were collected subject estimate PON1.All showed significantly lower both PON1 serum than controls (p < 0.001).An association between oxidative stress...

Resveratrol (RSV), a polyphenolic compound and naturally occurring phytoalexin, has been reported to exert cardio-protective effects in several animal studies. However, the outcome of initial clinical trials with RSV was less effective compared pre-clinical Therefore, treatment protocols need be optimized. In this study we evaluated prophylactic versus therapeutic effect resveratrol (RSV) mitigating doxorubicin (Dox)-induced cardiac toxicity rats. To investigate effects, supplemented for 2...

Vitamin D deficiency and vitamin receptor (VDR) gene polymorphisms have been linked to type 2 diabetes mellitus (T2DM) its metabolic parameters, however there are conflicting results therefore we aimed evaluate VDR (Fok1, Bsm1 Taq1) status in Egyptian patients with T2DM detect the associations of these their parameters glycemic control.