Progress in the study of Covid-19 disease rodents has been hampered by lack angiotensin-converting enzyme 2 (ACE2; virus entry route to target cell) affinities for spike proteins across species. Therefore, we sought determine whether a modified protocol lipopolysaccharide (LPS)-induced acute respiratory distress syndrome rats can mimic both cell signalling pathways as well severe phenotypes disease. Rats were injected via intratracheal (IT) instillation with either 15 mg/kg LPS (model group)...

Bone marrow-derived mesenchymal stem cells (BM-MSCs) have demonstrated potential in treating diabetic cardiomyopathy. However, patients with diabetes are on multiple drugs and there is a lack of understanding how transplanted would respond presence such drugs. Metformin an AMP kinase (AMPK) activator, the widest used antidiabetic drug. In this study, we investigated effect metformin efficacy cell therapy cardiomyopathy animal model using streptozotocin (STZ) male Wistar rats. To comprehend...

Diabetes is the most common cause of end-stage renal disease, also called kidney failure. The link between artery receptor angiotensin II type I (AT1R) and endothelin-1 (ET-1), involved in vasoconstriction, oxidative stress, inflammation fibrosis (collagen) diabetes-induced nephropathy with without metformin incorporation has not been previously studied. (type 2) was induced rats another group started (200 mg/kg) treatment 2 weeks prior to induction diabetes continued on until being culled...

Autism spectrum disorder (ASD) is a collective neurodevelopmental affecting young children and accounting for 1% of the world’s population. The cerebellum major part human brain affected by ASD associated with substantial reduction in number Purkinje cells. An association between expression nitrosative stress biomarker inducible nitric oxide synthase (iNOS), as well glycogen deposition damaged cells, has not been previously reported medical literature. To explore this correlation, rats were...

Lead is a common heavy metal used in variety of products. We investigated the effect lead-oxide nanoparticles (PbO-NPs) on histological structure rat olfactory bulb (OB) and potential therapeutic stem cell-derived exosomes (EXOs). Forty male rats were included: 8 for EXO isolation 32 distributed into 4 groups: control, PbO-NPs group [intranasal (IN) 50 µL PbO-NPs/rat 3 weeks], recovery (PbO-NPs weeks left another weeks), PbO-NP-EXO then IN weeks). After 7 weeks, behavioral tests done before...

Abstract Both hydrogen sulfide (H 2 S) and mesenchymal stem cells (MSCs) extracted microvesicles (MVs) are potent anti‐inflammatory molecules. They play an essential role in lowering the production of tumor necrosis factor‐alpha (TNF‐α). The latter could strongly stimulate MiR‐155 that contributes to neurodegeneration Alzheimer's disease (AD). miR‐155 repress expression inositol 5‐phosphatase‐1 (SHIP‐1) leading eventually activation Akt kinase neurofibrillary development AD. current study...

Wnt/β-catenin signaling pathway plays a crucial role in diabetic cardiomyopathy (DCM), thus we aimed at investigating the effect of one therapeutic approach with resveratrol (RSV) given systemically and combined treatment RSV mesenchymal stem cells (MSCs) that was either RSV-preconditioned or not on streptozotocin-induced DCM, to evaluate effects preconditioning MSCs potential. The rats were divided into control (C, n = 8), (DM, treated systemic (DM-RSV, nonconditioned (DM-RSV-MSCs,...

Background Vulvovaginal candidiasis (VVC) is a highly prevalent illness affecting women globally. Lactobacilli, which make up the majority of healthy vaginal microbiota (VMB), serve as powerful barrier against infections. Probiotic therapy has been recommended for treatment or prevention VVC. Aim work To compare in vivo therapeutic effects Lactobacillus johnsonii (B-2178) vs. acidophilus (LA-5 ® ) on VVC rat model, particularly highlighting immune response host epithelium. Methods In total,...

Allogeneic mesenchymal stem cells (MSCs) from young and healthy donors are immunoprivileged have the potential to treat numerous degenerative diseases. However, recent reviews of clinical trials report poor long-term survival transplanted in recipient that turned down enthusiasm regarding MSC therapies. Increasing evidence now confirm though initially immunoprivileged, MSCs eventually become immunogenic after transplantation ischemic or hypoxic environment diseased tissues rejected by host...

Cholestasis caused by slowing or blockage of bile flow is a serious liver disease that can lead to fibrosis and cirrhosis. The link between transforming growth factor beta 1 (TGFβ1), Smad family member 3 (Smad3), microRNA 21 (miR21) in duct ligation (BDL)-induced the presence absence anti-inflammatory antioxidant compound, resveratrol (RSV), has not been previously studied. Therefore, we tested whether RSV protect against BDL-induced associated with inhibition TGFβ1-Smad3-miR21 axis...

Liver fibrosis is a hallmark of thioacetamide (TAA) intoxications. MicroRNAs (miRs), such as miR-155, have been implied in the pathogenesis liver disease, and regulated by antioxidant anti-inflammatory compound resveratrol (RES). The link between reactive oxygen species (ROS), tumour suppressor p53 (p53), fibrosis-during TAA-induced injury-associated with miR-155 dysregulation without RES incorporation has not previously studied. Therefore, one group rats received TAA injections 200 mg/kg;...

Abstract Osteoarthritis is a debilitating, progressive joint disease linked to lower quality of life and higher health care costs. This study compared hyaluronic acid-chitosan nanoparticle encapsulation hyaluronic-acid monotherapy in rat model knee osteoarthritis. Four groups 40 adult male albino rats were designed. Group (Gp) I: control; Gp II (osteoarthritis model): intra-articular injection monoiodoacetate; III (hyaluronic acid-treated): injections on days 14 21 after monoiodoacetate...

Most of the drugs used for trichinellosis treatment showed a limited bioavailability, high degree resistance and weak activity against encapsulated larvae.Therefore, there is an urgent need to develop new agents improve bioavailability these drugs.So, aim present study was assess use chitosan (CH) nanoparticles alone or loaded with full half dose albendazole (ABZ) increase dissolution rate, enhance its antiparasitic during muscular phases T. spiralis infection.Fifty male albino mice were...

Toxic chemicals such as carbon tetrachloride and thioacetamide (TAA) are reported to induce hepato-nephrotoxicity. The potential protective outcome of the antidiabetic pleiotropic drug metformin against TAA-induced chronic kidney disease in association with modulation AMP-activated protein kinase (AMPK), oxidative stress, inflammation, dyslipidemia, systemic hypertension has not been investigated before. Therefore, 200 mg/kg TAA was injected (via intraperitoneal route) a model group rats...

Allogeneic mesenchymal stem cells (MSCs) from young and healthy donors are reported to hold the potential treat several immunological degenerative disorders. However, recent data animal studies clinical trials demonstrate that immunogenicity poor survival of transplanted MSCs impaired efficacy for regenerative applications. It is initially immunoprivileged under in vitro conditions, targeted by host immune system after transplantation ischemic tissues vivo. We performed (in MSCs) vivo rat...

Background Preeclampsia is a systemic, multi-organ endotheliopathy, associated with oxidative injury to the blood–brain barrier (BBB). initiates cascade of events that include neuroinflammation. Recently, it was documented Wnt/β-catenin signaling pathway exerts neuroprotective effects and maintain BBB integrity. We investigate protective effect omega-3 against neurovascular complication preeclampsia its relation pathway. Methodology After confirmation day 0 pregnancy (G0), 24 adult pregnant...

Autism is a neurodevelopmental disorder that impairs communication and social interaction. This study investigated the possible beneficial effects of erythropoietin (EPO) on experimental autistic-like behaviors induced by propionic acid (PPA). Twenty-four rats were distributed into three groups: (i) control; (ii) PPA_Gp: daily injected subcutaneously with PPA for five consecutive days; PPA+EPO-Gp: PPA, then received intraperitoneal injection EPO once two weeks. Behavioral changes in...

Context: Maternal diet composition could influence fetal organogenesis. Objective: We investigated effects of high fat (HFD) intake alone or combined with omega 3 during pregnancy, lactation and early days weaning on nephrogenesis pups maternal renal function morphology. Material methods: Mothers their included in each group were supplied the same composition. Rats divided into I, II III chow either 10 kcal%, 45 kcal% from together omega-3 respectively. Results: Group showed increased serum...