A5006411537- Pancreatic function and diabetes
- Pluripotent Stem Cells Research
- Mitochondrial Function and Pathology
- Metabolism, Diabetes, and Cancer
- Diabetes and associated disorders
- Tissue Engineering and Regenerative Medicine
- MXene and MAX Phase Materials
- Alkaline Phosphatase Research Studies
- Diabetes Management and Research
- Advanced Nanomaterials in Catalysis
- Mesenchymal stem cell research
- Biochemical and Molecular Research
- Metabolism and Genetic Disorders
- Diet and metabolism studies
- Phytochemicals and Antioxidant Activities
- Congenital heart defects research
- Graphene and Nanomaterials Applications
- Electrospun Nanofibers in Biomedical Applications
- ATP Synthase and ATPases Research
- Cardiac Fibrosis and Remodeling
- CRISPR and Genetic Engineering
- Natural Antidiabetic Agents Studies
- Vitamin D Research Studies
- Nosocomial Infections in ICU
- Tryptophan and brain disorders
University of Manitoba
2019-2024
St. Boniface Hospital
2019-2024
All India Institute of Medical Sciences
2011-2024
Maharaja Sayajirao University of Baroda
2013-2022
Institute of Medical Sciences
2015-2018
Abstract MXene nanomaterials have sparked significant interest among interdisciplinary researchers to tackle today's medical challenges. In particular, colloidal quantum dots (MQDs) offer the high specific surface area and compositional flexibility of while providing improvements aqueous stability material–cell interactions. The current study for first time reports development application immunoengineered tantalum‐carbide (Ta 4 C 3 T x ) MQDs in vivo treatment transplant vasculopathy. This...
Bone marrow-derived mesenchymal stem cells (BM-MSCs) have demonstrated potential in treating diabetic cardiomyopathy. However, patients with diabetes are on multiple drugs and there is a lack of understanding how transplanted would respond presence such drugs. Metformin an AMP kinase (AMPK) activator, the widest used antidiabetic drug. In this study, we investigated effect metformin efficacy cell therapy cardiomyopathy animal model using streptozotocin (STZ) male Wistar rats. To comprehend...
A “Leap-of-Faith” approach is used to treat patients with previously unknown ultrarare pathogenic mutations, often based on evidence from having dissimilar but more prevalent mutations. This uncertainty reflects the need develop personalized prescreening platforms for these assess drug efficacy before considering clinical trial enrollment. In this study, we report an 18-year-old patient Leigh-like syndrome. had participated in two trials unfavorable responses. We established induced...
Cardiovascular disease is a chronic multifactorial health complication that either directly or indirectly associated with pathophysiological mechanisms, including pro-oxidation, pro-inflammation, vascular and endothelial dysfunction, impaired platelet function, thrombosis, others. The therapeutic options to circumvent cardiovascular complications include several phytomedicines, green tea polyphenols. However, while many experimental clinical studies report distinct mechanisms by which the...
Transplanting islets serves best option for restoring lost beta cell mass and function. Small bio-chemical agents do have the potential to generate new mass, however lack of understanding about mechanistic action these small molecules eventually restricts their use in cell-based therapies diabetes. We recently reported "Swertisin" as a novel islet differentiation inducer, generating cells more effectively. Henceforth, present study we attempted investigate molecular signals that Swertisin...
Allogeneic mesenchymal stem cells (MSCs) are immunoprivileged and being investigated in phase I II clinical trials to treat different degenerative autoimmune diseases. In spite of encouraging outcome initial trials, the long-term poor survival transplanted host tissue has declined overall enthusiasm. Recent analyses allogeneic MSCs based studies confirm that after transplantation hypoxic or ischemic microenvironment diseased tissues, become immunogenic rejected by recipient immune system....
Aim . Stem cell therapy is one of the upcoming therapies for treatment diabetes. Discovery potent differentiating agents a prerequisite increasing islet mass. The present study an attempt to screen potential novel small biomolecules their property into pancreatic cells using NIH3T3, as representative extra stem cells/progenitors. Methods To identify new that stimulate differentiation, we screened various compounds isolated from Enicostemma littorale NIH3T3 and morphological changes were...
Kearns Sayre syndrome (KSS) is mitochondrial multisystem disorder with no proven effective treatment. The underlying cause for involvement the energy deficit resulting from load of mutant DNA (mtDNA), which manifests as loss cells and tissue dysfunction. Therefore, functional organ or cellular replacement provides a promising avenue therapeutic option. Patient-specific induced pluripotent stem (iPSC) have become handy tool to create personalized cell -based therapies. iPSC are capable...
Abstract Background Despite the potential, bone marrow-derived mesenchymal stem cells (BMSCs) show limitations for beta (ß)-cell replacement therapy due to inefficient methods deliver BMSCs into pancreatic lineage. In this study, we report TGF-ß family member protein, Activin-a potential stimulate efficient migration, enhanced homing and accelerated ß-cell differentiation. Methods Lineage tracing of permanent green fluorescent protein (GFP)- tagged donor murine transplanted either alone or...
Background: Tuberculosis (TB) and malnutrition are major global health problems, with multidrug-resistant (MDR) TB complicating international efforts. The role of vitamin D in susceptibility to as an adjunctive treatment for is being studied extensively, although no study has included MDR-TB patients context dietary profile levels sunlight exposure.
The purpose of this research work was to evaluate Piper betle ethyl acetate extract (PBEA) for its free radical scavenging, antioxidant, anti-apoptotic activities and role in protecting against oxidative cardiac cell injury.The Free scavenging activity antioxidant potential PBEA were evaluated using various non-cellular methods (1,1-Diphenyl-2-picrylhydrazyl, β-carotene bleaching, superoxide anion, hydroxyl radical, hydrogen peroxide, Reducing power, Total phenolics flavonoids). standardized...
Context Oxidative stress induces apoptosis within Islets of Langerhans in diabetes mellitus (DM). Enicostemma littorale blume, herb the Gentianaceae family is used as an anti-diabetic agent across rural India. Objective This report demonstrates potent anti-apoptotic and cyto-protective activity MeOH extract (EL ext.) against 50 μM H2O2 isolated rat Islets. Materials methods In this study, whole plant methanolic EL with doses 0.25-4 mg/mL each for preincubation duration 0.5-4 h were tested...
Kearns-Sayre syndrome (KSS) is an ultrarare multi-organ disorder, with a frequency of ~1 in 100,000 individuals. KSS characterized (1.1–10 kilobase) deletion mitochondrial DNA (mtDNA). We created induced pluripotent stem cell (iPSC) line from peripheral blood mononuclear cells (PBMCs) patient mtDNA 7.3 kilobase fragment. Heteroplasmy PBMCs provides novel opportunity to generate iPSC normal that can be used develop specific replacement therapies future. Hence, this unique was study phenotype...
Pluripotency and stemness is believed to be associated with high Oct-3/4, Nanog, Sox-2 (ONS) expression. Similar embryonic stem cells (ESCs), ONS expression eventually became the measure of pluripotency in any cell. The threshold genes that underscores pluripotency, stemness, differentiation potential still unclear. Therefore, we raised a question as whether function basal gene To prove this, carried out comparative study between expressing NIH3T3 pluripotent mouse bone marrow mesenchymal...
Abstract Poly(ADP-ribose) polymerase-1 (PARP1), a fundamental DNA repair enzyme, is known to regulate β cell death, replication, and insulin secretion. PARP1 knockout (KO) mice are resistant diabetes, while overactivation contributes death. Additionally, inhibition (PARPi) improves diabetes complications in patients with type-2 diabetes. Despite these beneficial effects, the use of modulating agents treatment largely neglected, primarily due poorly studied mechanistic action catalytic...
Abstract Pancreatic progenitors have been explored for their profound characteristics and unique commitment to generate new functional islets in regenerative medicine. resident endocrine (PREPs) with mesenchymal stem cell (MSC) phenotype were purified from BALB/c mice pancreas characterized. PREPs differentiated into mature islet clusters vitro by activin‐A swertisin functionally A temporal gene protein profiling was performed during differentiation. Furthermore, labeled green fluorescent...
Induced pluripotent stem cell (iPSC) line was derived from peripheral blood mononuclear cells (PBMCs) of a Kearns-Sayre syndrome (KSS) patient with mtDNA deletion 4.8 kilobase fragment. KSS is an ultrarare multi-organ disorder and characterized (1.1 to 10 kilobase) mitochondrial DNA (mtDNA) frequency ~1 in 100,000 individuals. Heteroplasmy PBMCs allowed us generate iPSC normal that can be used study therapeutic prospective their derivatives design future replacement therapies.
Abstract Chemoresistance is a major clinical challenge in the management of glioblastoma (GBM) Temozolomide (TMZ) chemotherapeutic drug choice for GBM; however, therapeutic effect TMZ limited due to development resistance. Recapitulating GBM chemoresistance controlled environment thus essential understanding mechanism chemoresistance. Herein, we present hybrid microphysiological model chemoresistant GBM-on-a-chip (HGoC) by directly co-culturing TMZ-resistant spheroids with healthy neurons...
Patients born with complex congenital heart defects, not amenable for surgical repair establishing normal bi-ventricular circulation are palliated the Fontan Circulation (FC). Here, we report new iPSC line from a patient tricuspid and pulmonary atresia. The underwent series of surgeries leading to completion at age 13yr., this was generated when she 38yr. old. exact genetic cause patient's defect is unknown, will be used studying molecular cellular mechanisms responsible cardiac dysfunction,...