A5007710641- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Rheumatoid Arthritis Research and Therapies
- Autophagy in Disease and Therapy
- Circular RNAs in diseases
- Pain Mechanisms and Treatments
- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Adenosine and Purinergic Signaling
- Osteoarthritis Treatment and Mechanisms
- Autoimmune and Inflammatory Disorders Research
- Spine and Intervertebral Disc Pathology
- RNA Research and Splicing
- Cytokine Signaling Pathways and Interactions
- Peptidase Inhibition and Analysis
- Biochemical and Molecular Research
- Ferroptosis and cancer prognosis
- Intensive Care Unit Cognitive Disorders
- Healthcare and Venom Research
- Chronic Lymphocytic Leukemia Research
- Monoclonal and Polyclonal Antibodies Research
- Ion Channels and Receptors
- Pregnancy-related medical research
- Systemic Lupus Erythematosus Research
- Hereditary Neurological Disorders
Xi'an Honghui Hospital
2015-2024
Xi'an Jiaotong University
2015-2024
Shaanxi Provincial People's Hospital
2023
Medical College of Wisconsin
2018-2022
Hong Kong Association of Registered Tour Co-ordinators
2020
First Affiliated Hospital of Xi'an Jiaotong University
2016-2019
Shandong Provincial QianFoShan Hospital
2019
Shandong University
2019
Clinical Orthopaedics and Related Research
2019
National Natural Science Foundation of China
2019
Objective Osteosarcoma (OS) is a common bone malignancy with poor prognosis. We aimed to investigate the relationship between cuproptosis-related lncRNAs (CRLncs) and survival outcomes of patients OS. Methods Transcriptome clinical data 86 OS were downloaded from The Cancer Genome Atlas (TCGA). GSE16088 dataset was Gene Expression Omnibus (GEO) database. 10 genes (CRGs) obtained recently published article on cuproptosis in Science . Combined analysis transcriptome identified differentially...
Rheumatoid arthritis fibroblast-like synovial cells (RA-FLS) show resistance to methotrexate (MTX) treatment. To better understand the mechanisms of this resistance, RA-FLS and osteoarthritis (OA-FLS) were isolated exposed MTX. We analyzed autophagy induced by MTX in vitro its relationship apoptosis.Cell viability was evaluated using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, apoptosis detected flow cytometry Western blot analysis. Autophagy determined transmission...
Abstract Micro RNA s are considered to play critical roles in the pathogenesis of human inflammatory arthritis, including rheumatoid arthritis ( RA ). The purpose this study was determine relationship between miR‐10a‐5p and TBX 5 synoviocytes evaluate their contribution joint inflammation. expression synovium synovial sarcoma cell line SW 982 stimulated by IL ‐1β determined RT ‐ qPCR Western blotting. direct interaction 3′ UTR dual‐luciferase reporter assay HeLa cells. Mimics inhibitors were...
Exosomes derived from mesenchymal stromal cells (MSCs) have emerged as novel drug and gene delivery tools. Current study aimed to elucidate the potential therapeutic role of human placental MSC (hPLMSC)-derived exosomes carrying AntagomiR-4450 (EXO-AntagomiR-4450) in intervertebral disc degeneration (IDD) progression. Initially, differentially expressed miRNAs related IDD were identified by microarray analysis, which provided data predicting interaction between microRNA-4450 (miR-4450) zinc...
Transient receptor potential ankyrin 1 (TRPA1) is well documented as an important molecule in pain hypersensitivity following inflammation and nerve injury many other cellular biological processes. Here, we show that TRPA1 expressed not only by sensory neurons of the dorsal root ganglia (DRG) but also their adjacent satellite glial cells (SGCs), nonmyelinating Schwann cells. immunoreactivity detected various cutaneous structures neuronal terminals, including small large caliber fibers...
Beclin1 is an important autophagy‑related protein, which involved in both autophagy and apoptosis. In recent years, the antitumor effect of has received increased attention. present study, we established a stable Beclin1‑overexpressing cell line with SW982 human synovial sarcoma cells. We found that overexpression decreased viability, inhibited proliferation induced apoptosis The expression levels Bcl‑2 PCNA were decreased, while cleaved‑caspase‑3 cleaved‑PARP increased. closely related...
We previously found that high-mobility group box protein 1 (HMGB1) promoted cell proliferation, migration, invasion, and autophagy in rheumatoid arthritis fibroblast-like synoviocytes (RA-FLS), but little is known about its regulatory mechanism. The aim of this study was to investigate the mechanism HMGB1 at posttranscription level. Real-time qPCR, CCK-8 proliferation assay, transwell migration enzyme-linked immunosorbent assay (ELISA), western blotting were used study. targeting...
Delirium is a common postoperative complication in elderly hip fracture patients that seriously affects patients' lives and health, early delirium risk prediction, targeted measures can significantly reduce the incidence of delirium. The purpose this study was to develop validate nomogram for prediction (POD) patients. A total 328 with fractures enrolled retrospectively department 1 our hospital were randomly divided into training set (n = 230) internal validation 98). least absolute...
The monosodium iodoacetate knee osteoarthritis model has been widely used for the evaluation of pain, but pathogenesis associated chronic pain is not fully understood. T-type calcium channel 3.2 (Ca V 3.2) abundantly expressed in primary sensory neurons, which it regulates neuronal excitability at both somata and peripheral terminals facilitates spontaneous neurotransmitter release spinal terminals. In this study, we investigated involvement neuron-Ca activation pain. Knee joint was induced...
Osteoarthritis (OA) is one of the most serious age-related diseases worldwide that drastically affects quality life patients. Despite advancements in treatment arthritis, especially with adipose-derived mesenchymal stem cells (ADSCs), senescence-induced alterations ADSCs negatively affect outcomes. This study was aimed at mechanistically exploring whether metformin could ameliorate senescence and effect metformin-preconditioned an experimental OA mouse model. In this study, H2O2-induced ADSC...
Autoimmune diseases, such as rheumatoid arthritis (RA) and systemic lupus erythematous, are regulated by polymorphisms in genes contributing to the NOX2 complex. Mutations both Ncf1 Ncf4 affect development of experimental models RA, but different regulatory pathways mediated NOX2-derived reactive oxygen species (ROS) have not yet been clarified. Here we address possibility that intracellular ROS, NCF4 protein (earlier often denoted p40phox) which interacts with endosomal membranes, could...
An imbalance between M1 and M2 macrophage polarization is critical in osteoarthritis (OA) development. We investigated the effect of macrophage-derived extracellular vesicles (M2-EVs) to reprogramme macrophages from phenotype for OA treatment. mouse models were treated with M2-EVs. Proteomic analysis was performed evaluate vitro. The as follows: destabilization medial meniscus (DMM) surgery-induced collagenase-induced (CIOA). Hyaluronic acid (HA) used deliver M2-EVs decreased accumulation,...
Abstract Objective The disruption of metabolic events and changes to nutrient oxygen availability due sustained inflammation in RA increases the demand bioenergetic biosynthetic processes within damaged tissue. current study aimed understand molecular mechanisms SLC7A5 (amino acid transporter) synoviocytes patients. Methods Synovial tissues were obtained from OA Fibroblast-like (FLS) isolated, expression was examined by using RT-qPCR, immunofluorescence, Western blotting. RNAi antibody...
Tandem mass tag (TMT)-coupled liquid chromatography coupled with tandem spectrometry is a powerful method to investigate synovial tissue protein profiles in patients rheumatoid arthritis (RA) and osteoarthritis (OA). Protein was isolated from samples of 22 labeled TMT kit. Over 500 proteins were identified as the differential expression on comparing RA OA tissue, including 239 upregulated 271 downregulated proteins. Data are available via ProteomeXchange identifier PXD027703. Gene ontology...
The goal of this study was to explore the expression characteristics RNA modification-related genes, reveal immune landscapes and identify novel potential diagnostic biomarkers in osteoarthritis (OA) rheumatoid arthritis (RA) patients.RNA microarray single-cell sequencing (scRNA-seq) data were downloaded from gene omnibus (GEO) database. Differentially expressed genes identified then functionally annotated. Univariate logistic regression lasso analysis used primary disease for OA RA....
Abstract Oxymatrine (OMT) is a type of alkaloid extracted from traditional Chinese medicinal herb, Sophora flavescens . Although the antitumor activities OMT have been observed in various cancers, there are no reports regarding effects on human synovial sarcoma. In present study, we analyzed SW982 sarcoma cells and determine whether high mobility group box protein 1 (HMGB1)-mediated autophagy was associated with its therapeutic effects. We found that exhibited activity facilitated increases...
To study pyroptosis-related biomarkers that are associated with the prognosis and immune microenvironment characteristics of osteosarcoma (OS). The goal is to establish a foundation for treatment OS.We retrieved transcriptome clinical data from Cancer Genome Atlas (TCGA) database 88 OS patients. Using this data, we constructed prognostic model identify genes (PRGs) prognosis. further explore biological function these PRGs, performed enrichment analysis. long non-coding RNAs (PRLncs) OS,...
Tmem100 modulates interactions between TRPA1 and TRPV1. The cell specificity of expression in dorsal root ganglia (DRGs) is not well defined, nor the effect peripheral nerve injury on expression.This study was designed to determine DRG its subcellular localization, examine how may be altered painful conditions.Dorsal ganglion determined by immunohistochemistry, immunoblot, quantitative real-time PCR, compared various experimental rat pain models controls.Tmem100 expressed both neurons...
Rheumatoid arthritis (RA) is a nonspecific, chronic, systemic autoimmune disease characterized by symmetric polyarticular synovitis. Bioinformatics analysis of potential biomarkers, mRNA-miRNA-lncRNA axes, and signaling pathways in the pathogenesis RA provides targets theoretical basis for further research on RA.The GSE1919 GSE77298 datasets were downloaded from Gene Expression Omnibus database (http://www.ncbi.nlm.nih.gov/geo). Perl was used to perform data merging, R batch correction. The...