A5062077025- Neurobiology and Insect Physiology Research
- Insect-Plant Interactions and Control
- Insect behavior and control techniques
- Insect Utilization and Effects
- Hemiptera Insect Studies
- Silkworms and Sericulture Research
- Ion channel regulation and function
- Silk-based biomaterials and applications
- Insect and Arachnid Ecology and Behavior
- Protein Kinase Regulation and GTPase Signaling
- Calcium signaling and nucleotide metabolism
- Educational Innovations and Challenges
- Ion Channels and Receptors
- Psychology of Development and Education
- MicroRNA in disease regulation
- Higher Education Learning Practices
- Invertebrate Immune Response Mechanisms
- Educational and Psychological Assessments
- Insect and Pesticide Research
- Marine Biology and Environmental Chemistry
- Evaluation of Teaching Practices
- Marine and coastal plant biology
- Phosphodiesterase function and regulation
- Protein Hydrolysis and Bioactive Peptides
- Cellular transport and secretion
National and Kapodistrian University of Athens
2011-2024
Alexander Fleming Biomedical Sciences Research Center
2016
Biomedical Research Foundation of the Academy of Athens
2016
Molecular Oncology (United States)
2016
University of Cambridge
2005-2011
Hospital Santa Caterina
2010
Bergen Kommune
2010
National Centre of Scientific Research "Demokritos"
2001-2009
Saxon Academy of Sciences and Humanities in Leipzig
2002-2008
University of Bath
2006
Inositol 1,4,5-trisphosphate receptors (IP3Rs) release calcium ions, Ca2+, from intracellular stores, but their roles in mediating Ca2+ entry are unclear. IP3 stimulated opening of very few (1.9 +/- 0.2 per cell) Ca2+-permeable channels whole-cell patch-clamp recording DT40 chicken or mouse B cells. Activation the cell receptor (BCR) perforated-patch recordings evoked same response. failed to stimulate plasma membrane (PM) cells lacking IP3R. Expression IP3R restored both responses....
Interactions between cyclic adenosine monophosphate (cAMP) and Ca2+ are widespread, for both intracellular messengers, their spatial organization is important. Parathyroid hormone (PTH) stimulates formation of cAMP sensitizes inositol 1,4,5-trisphosphate receptors (IP3R) to IP3. We show that PTH communicates with IP3R via “cAMP junctions” allow local delivery a supramaximal concentration IP3R, directly increasing sensitivity These junctions robust binary switches digitally recruited by...
In HEK cells stably expressing type 1 receptors for parathyroid hormone (PTH), PTH causes a sensitization of inositol 1,4,5-trisphosphate (IP(3)R) to IP(3) that is entirely mediated by cAMP and requires pass directly from 6 adenylyl cyclase (AC6) IP(3)R2. Using DT40 single subtypes mammalian IP(3)R, we demonstrate high concentrations similarly sensitize all IP(3)R isoforms mechanism does not require cAMP-dependent protein kinase (PKA). binding IP(3)R2 unaffected cAMP, the site through which...
Inositol 1,4,5-trisphosphate receptors (IP(3)R) within the endoplasmic reticulum mediate release of Ca(2+) from intracellular stores. Different channels usually entry across plasma membrane. In B lymphocytes and a cell line derived them (DT40 cells), very few functional IP(3)R (approximately 2/cell) are invariably expressed in membrane, where they about half evoked by activation B-cell receptor. We show that cells reliably count approximately 2 into membrane even when their conductance...
Ca2+ release by D-myo-inositol 1,4,5-trisphosphate receptors (IP3Rs) is widely considered to require the vicinal 4,5-bisphosphate motif of IP3, with P-5 and P-4 engaging α β domains binding site; using synthesis mutagenesis we show that adenine synthetic glyconucleotides, through an interaction Arg504, can replace either P-1 or α-domain producing, respectively, most potent bisphosphate agonist yet synthesised first IP3R without a motif; this will stimulate new approaches ligand design.
Red algae of the genus Laurencia are known to biosynthesize and secrete an immense variety secondary metabolites possessing a spectrum biological activities against bacteria, invertebrates mammalian cell lines. Following rigorous cross-species screening process, herein we report antifouling potential 25 derived from species Laurencia, as well thorough evaluation ecotoxicity selected non-target marine arthropods vertebrate A number these exhibited potent activity performed in all tests. Our...
The synthesis of adenophostin A (2) and two analogues [etheno (4) 8-bromo (5)] modified at the adenine moiety, is reported. combination NMR analysis molecular modelling was used to compare their structures in solution determined that they all adopt very similar conformations. were tested for ability mobilise Ca2+ from DT40 cells expressing recombinant Type 1 rat Ins(1,4,5)P3R which reveals etheno as a high affinity fluorescent probe Ins(1,4,5)P3R. 8-Bromo only slightly less potent....
Abstract Ecdysteroids secreted by the prothoracic gland (PG) cells of insects control developmental timing their immature life stages. These have been historically considered as carrying out a single function in insects, namely biochemical conversion cholesterol to ecdysteroids and secretion. A growing body evidence shows that PG receive multiple cues during insect development so we tested hypothesis they carry more than just one insects. We characterised molecular nature profiles cell...
The hush puppy mouse mutant has been shown previously to have skull and outer, middle, inner ear defects, an increase in hearing threshold. fibroblast growth factor receptor 1 (Fgfr1) gene is located the region of chromosome 8 containing mutation. Sequencing heterozygotes revealed a missense mutation kinase domain protein (W691R). Homozygotes were found die during development, at approximately embryonic day 8.5, displayed phenotype similar null mutants. Reverse transcription PCR indicated...
Reproduction in barnacles relies on chemical cues that guide their gregarious settlement. These have been pinned down to several sources of settlement pheromones, one which is a protein termed Settlement-Inducing Protein Complex (SIPC), large glycoprotein acting as pheromone induce larval and an adhesive the surface exploration by cyprids. Settlement assays laboratory conditions with Amphibalanus (=Balanus) amphitrite cyprids presence SIPC showed exhibit preference behaviour at lower...
Previous structure-activity relationship studies of adenophostin A, a potent IP(3) receptor agonist, led us to design the novel A analogues 5a-c, conjugating an aromatic group at 5'-position develop useful ligands. The common key intermediate, D-ribosyl alpha-D-glucoside 10alpha, was stereoselectively synthesized by glycosidation with 1-sulfinylglucoside donor 11, which conformationally restricted 3,4-O-cyclic diketal protecting group. After introduction 5-position ribose moiety, adenine...
To describe newly identified autoantibodies associated with cerebellar disorders.We first screened the sera of 15 patients ataxia, without any known autoantibodies, immunocytochemistry on mouse brain. After characterization and validation a antibody, 85 additional suspected autoimmune disease were using cell-based assay.Immunoglobulin G from one demonstrated distinct staining pattern Purkinje neurons. This autoantibody, as characterized further by immunoprecipitation mass spectrometry, was...
The TREK family of leak potassium channels has been found to play critical roles in nociception, sensitivity general anaesthetics, neuroprotection, and memory. three members the family, TREK1, TREK2 TRAAK establish resting potential modify duration, frequency amplitude action potentials. Despite their apparent importance, repertoire regulatory interactions utilized by cells control expression is poorly understood. Herein, contribution miRNAs regulation post-transcriptional gene examined....
The prothoracic gland (PG), the principal steroidogenic organ of insects, has been proposed as a model for steroid hormone biosynthesis and regulation. To validate robustness model, we present an analysis accumulated transcriptomic data from PGs two species, Drosophila melanogaster Bombyx mori. We identify that common core components in both species are encoded by nine genes. Five these Halloween genes whose expression differs substantially between species. conclude can be synthesis...
β-C-Glucoside trisphosphates having a C2 side chain (3,7-anhydro-2-deoxy-d-glycero-d-gulo-octitol 1,5,6-trisphosphate, 11) and C3 (4,8-anhydro-2,3-dideoxy-d-glycero-d-gulo-nonanitol 1,6,7-trisphosphate, 12) were designed as structurally simplified analogues of potent d-myo-inositol 1,4,5-trisphosphate (IP3) receptor ligand, adenophostin A. Construction the β-C-glucosidic structure, which was key to their synthesis, achieved by two different methods based on conformational restriction...