A5052983312- Adipokines, Inflammation, and Metabolic Diseases
- Atherosclerosis and Cardiovascular Diseases
- Immune cells in cancer
- Phagocytosis and Immune Regulation
- Molecular Biology Techniques and Applications
- Single-cell and spatial transcriptomics
- Cell Image Analysis Techniques
- Cardiovascular Function and Risk Factors
- Microfluidic and Bio-sensing Technologies
- Cancer, Lipids, and Metabolism
- Metabolomics and Mass Spectrometry Studies
- Advanced Proteomics Techniques and Applications
- Cell Adhesion Molecules Research
- Cardiac Imaging and Diagnostics
- Cardiovascular Disease and Adiposity
- Cerebrovascular and Carotid Artery Diseases
Maastricht University
2021-2024
Maastricht University Medical Centre
2023
BACKGROUND: The metabolic alterations occurring within the arterial architecture during atherosclerosis development remain poorly understood, let alone those particular to each tunica. We aimed first identify, in a spatially resolved manner, specific changes plaque, media, adventitia, and cardiac tissue between control atherosclerotic murine aortas. Second, we assessed their translatability human plasma for cardiovascular risk estimation. METHODS: In this observational study, mass...
Abstract Macrophage infiltration and accumulation in the atherosclerotic lesion are associated with plaque progression instability. Depletion of macrophages from might provide valuable insights into stabilization processes. Therefore, we assessed effects systemic local macrophage depletion on atherogenesis. To deplete monocytes/macrophages used atherosclerosis‐susceptible Apoe − /− mice, bearing a MaFIA (macrophage‐Fas‐induced‐apoptosis) suicide construct under control Csf1r (CD115)...
Recent technical advances, such as single-cell RNA sequencing and mass cytometry, improve identification of cell types subsets in a range healthy diseased tissues at the expense their cellular molecular context. Here, we present protocol for situ multispectral imaging to map myeloid heterogeneity tissue cryosections, describing steps cutting sequential sections, antibody titration, building spectral library. We then detail procedures preparing data downstream analysis. For complete details...