A5046047916- Atherosclerosis and Cardiovascular Diseases
- Immune cells in cancer
- Adipokines, Inflammation, and Metabolic Diseases
- Cancer, Lipids, and Metabolism
- Single-cell and spatial transcriptomics
- Advanced Proteomics Techniques and Applications
- Neuroinflammation and Neurodegeneration Mechanisms
- Cholesterol and Lipid Metabolism
- Metabolomics and Mass Spectrometry Studies
- Bioinformatics and Genomic Networks
- Phagocytosis and Immune Regulation
- Immune Cell Function and Interaction
- Mass Spectrometry Techniques and Applications
- Cell Adhesion Molecules Research
- Neurological Disease Mechanisms and Treatments
- Galectins and Cancer Biology
- Cell Image Analysis Techniques
- Parasites and Host Interactions
- Cytokine Signaling Pathways and Interactions
- Cardiovascular Disease and Adiposity
- Biomarkers in Disease Mechanisms
- Cardiac Imaging and Diagnostics
- Immune Response and Inflammation
- Systemic Lupus Erythematosus Research
- Barrier Structure and Function Studies
Maastricht University
2013-2024
Maastricht University Medical Centre
2019-2024
University Medical Center
2023
Aix-Marseille Université
2013-2021
Inserm
2018-2021
Centre National de la Recherche Scientifique
2019
Centre d’Immunologie de Marseille-Luminy
2013-2017
Abstract Monocytes circulate the vasculature at steady state and are recruited to sites of inflammation where they differentiate into macrophages (MФ) replenish tissue-resident MФ populations engage in development cardiovascular disease (CVD). display considerable heterogeneity, currently reflected by a nomenclature based on their expression cluster differentiation (CD) 14 CD16, distinguishing CD14++CD16− classical (cMo), CD14++CD16+ intermediate (intMo) CD14+CD16++ non-classical (ncMo)...
Atherosclerosis is a lipid-driven inflammatory disease of the vessel wall, characterized by chronic activation macrophages.We investigated whether helminth-derived antigens [soluble egg (SEAs)] could modulate macrophage responses and protect against atherosclerosis in mice.In bone marrow-derived macrophages, SEAs induce anti-inflammatory typified high levels IL-10 reduced secretion proinflammatory mediators.In hyperlipidemic LDLR ؊/؊ mice, SEA treatment plaque size 44%, plaques were less...
Rationale: Hypertension is a major risk factor for cerebral small vessel disease, the most prevalent cause of vascular cognitive impairment. As we have shown, hypertension induced by prolonged Angiotensin II infusion associated with increased permeability blood-brain barrier (BBB), chronic activation microglia and myelin loss. In this study therefore aim to determine contribution hypertension-induced impairment in an experimental model pharmacological depletion approach. Methods: For study,...
Cells often adopt different phenotypes, dictated by tissue-specific or local signals such as cell-cell and cell-matrix contacts molecular micro-environment. This holds in extremis for macrophages with their high phenotypic plasticity. Their broad range of functions, some even opposing, reflects heterogeneity, a multitude subsets has been described tissues diseases. Such micro-environmental imprint cannot be adequately studied single-cell applications, cells are detached from context, while...
Abstract Aims Specific fibroblast markers and in-depth heterogeneity analysis are currently lacking, hindering functional studies in cardiovascular diseases (CVDs). Here, we established cell-type murine human arteries studied the adventitial response to CVD its risk factors hypercholesterolaemia ageing. Methods results Murine aorta single-cell RNA-sequencing of mesenchymal cells identified fibroblast-specific markers. Immunohistochemistry flow cytometry validated platelet-derived growth...
BACKGROUND: The metabolic alterations occurring within the arterial architecture during atherosclerosis development remain poorly understood, let alone those particular to each tunica. We aimed first identify, in a spatially resolved manner, specific changes plaque, media, adventitia, and cardiac tissue between control atherosclerotic murine aortas. Second, we assessed their translatability human plasma for cardiovascular risk estimation. METHODS: In this observational study, mass...
Macrophages form a heterogeneous population of immune cells, which is critical for both the initiation and resolution inflammation. They can be skewed to proinflammatory subtype by Th1 cytokine IFN-γ further activated with TLR triggers, such as LPS. In this work, we investigated effects priming on LPS-induced gene expression in primary mouse macrophages. Surprisingly, found that represses subset genes, particularly genes involved cellular movement leukocyte recruitment. We STAT1-binding...
Abstract Background Metabolism is increasingly recognized as a key regulator of the function and phenotype primary cellular constituents atherosclerotic vascular wall, including endothelial cells, smooth muscle inflammatory cells. However, comprehensive analysis metabolic changes associated with transition plaque from stable to hemorrhaged lacking. Methods In this study, we integrated two large mRNA expression protein abundance datasets (BIKE, n = 126; MaasHPS, 43) human carotid artery...
This study aims to identify sex-specific transcriptional differences and signaling pathways in circulating monocytes contributing cardiovascular disease.We generated sex-biased gene expression signatures by comparing male versus female of coronary artery disease (CAD) patients (n = 450) from the Center for Translational Molecular Medicine-Circulating Cells Cohort. Gene set enrichment analysis demonstrated that CAD carry stronger chemotaxis migratory signature than those males. We then...
Activation of the transcription factor NF-κB appears to be involved in different stages atherogenesis. In this paper we investigate role inhibitor IκBα atherosclerosis. Myeloid-specific deletion results larger and more advanced lesions LDL-R-deficient mice without affecting compositional phenotype plaques or systemic inflammatory markers plasma. We show that IκBα-deleted macrophages display enhanced adhesion an vitro endothelial cell layer, coinciding with increased expression chemokine...
Atherosclerosis is the major contributor to cardiovascular diseases. It a spatially and temporally complex inflammatory disease, in which intravascular accumulation of plethora lipids considered play crucial role. To date, both composition local distribution involved have not been thoroughly mapped yet. Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) enables analyzing visualizing hundreds lipid molecules within plaque while preserving each lipid's specific...
Inflammatory responses and cholesterol homeostasis are interconnected in atherogenesis. Interleukin (IL)-10 is an important anti-inflammatory cytokine, known to suppress atherosclerosis development. However, the specific cell types responsible for atheroprotective effects of IL-10 remain be defined knowledge on actions scarce. Here we investigated functional involvement myeloid IL-10-mediated atheroprotection. To do so, bone marrow from receptor 1 (IL-10R1) wild-type IL-10R1-deficient mice...
Abstract Macrophage infiltration and accumulation in the atherosclerotic lesion are associated with plaque progression instability. Depletion of macrophages from might provide valuable insights into stabilization processes. Therefore, we assessed effects systemic local macrophage depletion on atherogenesis. To deplete monocytes/macrophages used atherosclerosis‐susceptible Apoe − /− mice, bearing a MaFIA (macrophage‐Fas‐induced‐apoptosis) suicide construct under control Csf1r (CD115)...
Delayed-type hypersensitivity (DTH) responses underpin chronic inflammation. Using a model of oxazolone-induced dermatitis and combination transgenic mice, adoptive cell transfer, selective agonists/antagonists against protease activated receptors, we show that PAR-1 signaling on macrophages by thrombin is required for effective granuloma formation. BM-derived (BMMs) in vitro, induced (a) downregulation membrane reverse cholesterol transporter ABCA1 (b) increased expression IFNγ receptor...
Inhibition of STAT5 was recently reported to reduce murine atherosclerosis. However, the role isoforms, and more in particular STAT5A macrophages context human atherosclerosis remains unknown.Here, we demonstrate reciprocal expression regulation STAT5B atherosclerotic lesions. The former highly upregulated ruptured over stable plaque correlated with macrophage presence, a finding that corroborated by high chromosomal accessibility but not B gene macrophages. Phosphorylated confirming its...
Macrophages (MΦ) are commonly cultured in vitro as a model of their biology and functions tissues. Recent evidence suggests MΦ to engage quorum sensing, adapting response cues about the proximity neighboring cells. However, culture density is frequently overlooked standardization protocols well interpretation results obtained . In this study, we investigated how functional phenotype was influenced by density. We assessed 10 core human derived from THP-1 cell line primary monocyte-derived MΦ....
Pathogens or trauma-derived danger signals induced maturation and activation of plasmacytoid dendritic cells (pDCs) is a pivotal step in pDC-dependent host defense. Exposure pDC to cardiometabolic disease-associated lipids proteins may well influence critical signaling pathways, thereby compromising immune responses against endogenous, bacterial viral pathogens. In this study, we have addressed if hyperlipidemia impacts human activation, cytokine response capacity prime CD4+ T cells.We show...