A5084317532- Biblical Studies and Interpretation
- Religion, Society, and Development
- Archaeology and Historical Studies
- Single-cell and spatial transcriptomics
- Atherosclerosis and Cardiovascular Diseases
- Aquaculture disease management and microbiota
- Immune Cell Function and Interaction
- MicroRNA in disease regulation
- Forest Insect Ecology and Management
- Plant Molecular Biology Research
- Chromosomal and Genetic Variations
- T-cell and B-cell Immunology
- Fish Ecology and Management Studies
- Angiogenesis and VEGF in Cancer
- Christian Theology and Mission
- Genomics and Phylogenetic Studies
- Liver physiology and pathology
- Coronary Interventions and Diagnostics
- Immune cells in cancer
- Congenital heart defects research
- Soil Moisture and Remote Sensing
- French Urban and Social Studies
- Cardiac Fibrosis and Remodeling
- Molecular Biology Techniques and Applications
- Geophysical and Geoelectrical Methods
Roslin Institute
2019-2025
University of Edinburgh
2018-2024
University of Manchester
2024
The Queen's Medical Research Institute
2018-2023
Atrium Health Wake Forest Baptist
2022
Centre for Inflammation Research
2018-2020
University of Bristol
2014-2019
York Teaching Hospital NHS Foundation Trust
2019
Foothills Medical Centre
2005
University of Calgary
2005
A better understanding of the pathways that regulate regeneration coronary vasculature is fundamental importance for advancement strategies to treat patients with heart disease. Here, we aimed investigate origin and clonal dynamics endothelial cells (ECs) associated neovascularization in adult mouse following myocardial infarction (MI). Furthermore, sought define murine cardiac heterogeneity characterize transcriptional profiles pro-angiogenic resident ECs heart, at single-cell resolution.
Single-cell transcriptomics is the current gold standard for global gene expression profiling, not only in mammals and model species, but also non-model fish species. This a rapidly expanding field, creating deeper understanding of tissue heterogeneity distinct functions individual cells, making it possible to explore complexities immunology on highly resolved level. In this study, we compared two single cell transcriptomic approaches investigate cellular within head kidney healthy farmed...
Rationale: Endothelial-to-mesenchymal transition (EndMT) is a dynamic biological process involved in pathological vascular remodeling. However, the molecular mechanisms that govern this remain largely unknown, including contribution of long noncoding RNAs (lncRNAs). Objectives: To investigate role lncRNAs EndMT and their relevance to Methods Results: study vitro, primary endothelial cells were treated with transforming growth factor-β2 interleukin-1β. Single-cell bulk RNA-seq...
The absence of germinal centers (GCs) in cartilaginous fishes lies at odds with data showing that nurse sharks can produce robust antigen-specific responses and affinity mature their B cell repertoires. To investigate this apparent incongruity, we performed RNA sequencing on single nuclei, allowing us to characterize the types present shark spleen, RNAscope provide situ cellular resolution key marker gene expression following immunization R-phycoerythrin (PE). We tracked PE splenic follicles...
Abstract Aims Specific fibroblast markers and in-depth heterogeneity analysis are currently lacking, hindering functional studies in cardiovascular diseases (CVDs). Here, we established cell-type murine human arteries studied the adventitial response to CVD its risk factors hypercholesterolaemia ageing. Methods results Murine aorta single-cell RNA-sequencing of mesenchymal cells identified fibroblast-specific markers. Immunohistochemistry flow cytometry validated platelet-derived growth...
The spleen is a conserved secondary lymphoid organ that emerged in parallel to adaptive immunity early jawed vertebrates. Recent studies have applied single cell transcriptomics reveal the cellular composition of several species, cataloguing diverse immune types and subpopulations. In this study, 51,119 nuclei transcriptomes were comprehensively investigated commercially important teleost Atlantic salmon (Salmo salar L.), contrasting control animals with those challenged bacterial pathogen...
There is currently convincing evidence that microRNAs have evolved independently in at least six different eukaryotic lineages: animals, land plants, chlorophyte green algae, demosponges, slime molds and brown algae. MicroRNAs from lineages are not homologous but some structural features strongly conserved across the tree allowing application of stringent criteria to identify novel microRNA loci. A large set 63 families was identified alga Ectocarpus based on mapping RNA-seq data nine were...
microRNAs are conserved noncoding regulatory factors implicated in diverse physiological and developmental processes multicellular organisms, as causal macroevolutionary agents for phylogeny inference. However, the conservation phylogenetic utility of has been questioned on evidence pervasive loss. Here, we show that apparent widespread losses are, largely, an artefact poorly sampled annotated microRNAomes. Using a curated data set animal microRNAomes, reject view miRNA families never lost,...
Pluripotent stem cell-derived endothelial cell products possess therapeutic potential in ischaemic vascular disease. However, the factors that drive differentiation from pluripotency and cellular specification are largely unknown. The aims of this study were to use single-cell RNA sequencing (scRNA-seq) map transcriptional landscape dynamics directed human embryonic cells (hESC-EC) compare these mature diverse beds.
The liver is a multitasking organ with essential functions for vertebrate health spanning metabolism and immunity. In contrast to mammals, our understanding of cellular heterogeneity its role in regulating immunological status remains poorly defined fishes. Addressing this knowledge gap, we generated transcriptomic atlas 47,432 nuclei isolated from the Atlantic salmon ( Salmo salar L.) contrasting control fish those challenged pathogenic strain Aeromonas salmonicida , problematic bacterial...
Viral replication in endothelial cells is a hallmark of many viral diseases humans and other animals, underscoring the importance understanding cellular mechanisms that restrict associated consequences for vascular health. Pathogenic variants infectious salmon anaemia virus (ISAV, Isavirus salaris) target Atlantic (Salmo salar L.), causing severe systemic disease major losses during outbreaks aquaculture. To better understand response to ISAV, we used single nucleus RNA-sequencing at...
Abstract Teasing apart the effects of natural selection and demography on current allele frequencies is challenging, due to both processes leaving a similar molecular footprint. In particular, when attempting identify in species that have undergone recent range expansion, increase genetic drift at edges expansions (“allele surfing”) can be confounding factor. To address this potential issue, we first assess long‐range colonization history Aleppo pine across Mediterranean Basin, using...
Abstract Congenital alveolar capillary dysplasia is a rare and fatal cause of pulmonary hypertension in neonates. We report on term baby with severe unresponsive to high‐frequency ventilation nitric oxide. A diagnosis was established autopsy. review the literature regarding pathophysiology, clinical presentations, associated malformations, treatment trials. Pediatr Pulmonol. © 2005 Wiley‐Liss, Inc.
Finding outlier loci underlying local adaptation is challenging and best approached by suitable sampling design rigorous method selection. In this study, we aimed to detect (single nucleotide polymorphisms, SNPs) at the scale using Aleppo pine (Pinus halepensis), a drought resistant conifer that has colonized many habitats in Mediterranean Basin, as model species. We used nested approach considered replicated altitudinal gradients for three contrasting sites. genotyped samples 294 SNPs...
The use of single cell sequencing technologies has exploded over recent years, and is now commonly used in many non-model species. Sequencing nuclei instead whole cells become increasingly popular, as it does not require the processing samples immediately after collection. Here we present a highly effective nucleus isolation protocol that outperforms previously available method challenging specie. This can be successfully applied to extract from variety tissues
Recent fate-mapping studies in mice have provided substantial evidence that mature adult hepatocytes are a major source of new after liver injury. In other systems, integrin αvβ8 has role activating transforming growth factor (TGF)-β, potent inhibitor hepatocyte proliferation. We hypothesized depletion would increase proliferation and accelerate regeneration Using Itgb8flox/flox;Alb-Cre to deplete αvβ8, partial hepatectomy, liver-to-body weight ratio were significantly increased compared...
Thurso/Caithness in the United Kingdom has gone through a lot of changes and transitions last decades. The decision to build nuclear reactor test facility 1950’s Dounreay/Caithness UK, as well current phase decommissioning impacted not only technology development but also social fabric community individuals within it. This study aimed explore lived experiences people by project at Dounreay. results will form basis for discussion about locating future experimental or facilities, possibly...
Background: Thurso/Caithness in the United Kingdom has gone through a lot of changes and transitions last decades. The decision to build nuclear reactor test facility 1950’s Dounreay/Caithness UK, as well current phase decommissioning impacted not only technology development but also social fabric community individuals within it. This study aimed explore lived experiences people by project at Dounreay. results will form basis for discussion about locating future experimental or facilities,...
Evaluation of targeted therapies is urgently needed for the majority patients with metastatic/recurrent head and neck squamous cell carcinoma (HNSCC) who progress after immunochemotherapy. Erlotinib, a inhibitor epidermal growth factor receptor pathway, lacks FDA approval in HNSCC due to inadequate tumor response. This study identifies two potential avenues improve response erlotinib among HNSCC. For first time, this shows that an increased dose 300 mg smokers well-tolerated produces similar...