A5104946755- Mosquito-borne diseases and control
- Viral Infections and Vectors
- HIV Research and Treatment
- interferon and immune responses
- Neonatal and fetal brain pathology
- Malaria Research and Control
- Viral Infections and Outbreaks Research
- Liver Disease Diagnosis and Treatment
- Alkaloids: synthesis and pharmacology
- Click Chemistry and Applications
- Biochemical and Molecular Research
- Computational Drug Discovery Methods
- Chemical synthesis and alkaloids
- Autophagy in Disease and Therapy
- Hepatitis C virus research
- Infectious Encephalopathies and Encephalitis
- RNA modifications and cancer
- Insect symbiosis and bacterial influences
- Vibrio bacteria research studies
Institut National de la Recherche Scientifique
2019-2024
Institut Pasteur de Dakar
2023
Armand Frappier Museum
2020
Dengue fever, caused by dengue virus (DENV), is the most prevalent arthropod-borne viral disease and endemic in many tropical subtropical parts of world, with an increasing incidence temperate regions. The closely related flavivirus Zika (ZIKV) can be transmitted vertically utero causes congenital syndrome other birth defects. In adults, ZIKV associated Guillain-Barré syndrome. There are no approved antiviral therapies against either virus. Effective compounds urgently needed. Amaryllidaceae...
Dengue virus (DENV) is a
Abstract Flaviviridae infections represent a major global health burden. By deciphering mechanistic aspects of hepatitis C virus (HCV)-host interactions, one could discover common strategy for inhibiting the replication related flaviviruses. elucidating HCV interactome, we identified 17-beta-hydroxysteroid dehydrogenase type 12 (HSD17B12) as human hub very-long-chain fatty acid (VLCFA) synthesis pathway and core interactor. Here show that HSD17B12 knockdown (KD) impairs reduces virion...
With no available therapies, infections with Zika virus (ZIKV) constitute a major public health concern as they can lead to congenital microcephaly. In order generate an intracellular environment favourable viral replication, ZIKV induces endomembrane remodelling and the morphogenesis of replication factories via enigmatic mechanisms. this study, we identified AAA+ type ATPase valosin-containing protein (VCP) cellular interaction partner non-structural 4B (NS4B). Importantly, its...
The dengue virus (DENV) causes the most prevalent arthropod-borne viral disease worldwide. While its incidence is increasing in many countries, there no approved antiviral therapy currently available. In infected cells, DENV induces extensive morphological alterations of endoplasmic reticulum (ER) to generate replication organelles (vRO), which include convoluted membranes (CM) and vesicle packets (VP) hosting RNA replication. non-structural protein NS4B localizes vROs absolutely required...
ABSTRACT With no therapeutics available, there is an urgent need to better understand the pathogenesis of flaviviruses which constitute a threat public health worldwide. During infection, dengue virus (DENV) and Zika (ZIKV), two induce alterations mitochondria morphology favor viral replication, suggesting co-opting functions. Here, we performed extensive transmission electron microscopy-based quantitative analysis demonstrate that both DENV ZIKV alter endoplasmic reticulum-mitochondria...
Fragment-based lead discovery has emerged over the last decades as one of most powerful techniques for identifying starting chemical matter to target specific proteins or nucleic acids in vitro. However, use such low-molecular-weight fragment molecules cell-based phenotypic assays been historically avoided because concerns that bioassays would be insufficiently sensitive detect limited potency expected small and high concentrations required likely implicate undesirable artifacts. Herein, we...
ABSTRACT Infection of pregnant women by Zika virus (ZIKV) is associated with severe neurodevelopmental defects in newborns through poorly defined mechanisms. Here, we engineered a zebrafish vivo model ZIKV infection to circumvent limitations existing mammalian models. Leveraging the unique tractability this system, gained unprecedented access ZIKV-infected brain at early developmental stages. The larvae phenocopied disease mammals including reduced head area and neural progenitor cells (NPC)...
Zika virus (ZIKV) infection causes significant human disease that, with no approved treatment or vaccine, constitutes a major public health concern. Its life cycle entirely relies on the cytoplasmic fate of viral RNA genome (vRNA) through fine-tuned equilibrium between vRNA translation, replication, and packaging into new virions, all within virus-induced replication organelles (vROs). In this study, an interference (RNAi) mini-screening subsequent functional characterization, we have...
Infection of pregnant women by Zika virus (ZIKV) is associated with severe neurodevelopmental defects in newborns through poorly defined mechanisms. Here, we established a zebrafish vivo model ZIKV infection to circumvent limitations existing mammalian models. Leveraging the unique tractability this system, gained unprecedented access ZIKV-infected brain at early developmental stages. The larvae phenocopied disease mammals including reduced head area and neural progenitor cells (NPC)...
Arthropod-borne diseases currently constitute a source of major health concerns worldwide. They account for about 50% global infectious and cause nearly 700,000 deaths every year. Their rapid increase spread huge challenge public health, highlighting the need early detection during epidemics, to curtail virus spread, enhance outbreak management. Here, we compared standard quantitative polymerase chain reaction (RT-qPCR) direct RT-qPCR assay Zika (ZIKV), Chikungunya (CHIKV), Rift Valley Fever...