A5036131817- Cancer, Hypoxia, and Metabolism
- Renal cell carcinoma treatment
- Single-cell and spatial transcriptomics
- Zebrafish Biomedical Research Applications
- RNA Research and Splicing
- Immune cells in cancer
- Bone Metabolism and Diseases
- Epigenetics and DNA Methylation
- Fungal Infections and Studies
- Blood disorders and treatments
- Receptor Mechanisms and Signaling
- Renal and related cancers
- Advanced Fluorescence Microscopy Techniques
- melanin and skin pigmentation
- Bone health and osteoporosis research
- Sirtuins and Resveratrol in Medicine
- Redox biology and oxidative stress
- Pluripotent Stem Cells Research
- Cancer-related gene regulation
- Adrenal and Paraganglionic Tumors
- Mitochondrial Function and Pathology
- CRISPR and Genetic Engineering
- Neurobiology and Insect Physiology Research
- Immunotherapy and Immune Responses
- Neutropenia and Cancer Infections
Dana-Farber Cancer Institute
2020-2024
Harvard University
2015-2024
Boston Children's Museum
2024
Boston Children's Hospital
2020-2024
Howard Hughes Medical Institute
2021-2024
Harvard Stem Cell Institute
2020
Center for Cancer Research
2015-2016
Massachusetts General Hospital
2015-2016
Charles River Associates
2015
Patients with a germline mutation in von Hippel-Lindau (VHL) develop renal cell cancers and hypervascular tumors of the brain, adrenal glands, pancreas as well erythrocytosis. These phenotypes are driven by aberrant expression HIF2α, which induces genes involved proliferation, angiogenesis, red blood production. Currently, there no effective treatments available for VHL disease. Here, using an animal model VHL, we report marked improvement VHL-associated following treatment HIF2α inhibitors....
Developmental signaling pathways associated with growth factors such as TGFb are commonly dysregulated in melanoma. Here we identified a human enhancer specifically activated melanoma cells treated TGFB1 ligand. We generated stable transgenic zebrafish this Induced Enhancer driving green fluorescent protein ( TIE:EGFP ). was not expressed normal melanocytes or early melanomas but spatially distinct regions of advanced melanomas. Single-cell RNA-sequencing revealed that + down-regulated...
ABSTRACT Patients with von Hippel–Lindau (VHL) disease harbor a germline mutation in the VHL gene leading to development of several tumor types including clear cell renal carcinoma (ccRCC). In addition, is inactivated over 90% sporadic ccRCC cases. ‘Clear cell’ tumors contain large, proliferating cells ‘clear cytoplasm’, and reduced number cilia. inactivation leads stabilization hypoxia inducible factors 1a 2a [HIF1a HIF2a (HIF2a also known as EPAS1)] consequent up-regulation specific target...
Zebrafish are rapidly becoming a leading model organism for cancer research. The genetic pathways driving highly conserved between zebrafish and humans, the ability to easily manipulate genome generate transgenic animals makes an excellent organism. Transgenic containing complex, patient-relevant genotypes have been used many types. Here we present comprehensive review of models as resource field highlight important areas biology that yet be studied in fish. image cells niche endogenous...
The field cancerization theory suggests that a group of cells containing oncogenic mutations are predisposed to transformation 1, 2 . We previously identified single in BRAF V600E ;p53 -/- zebrafish reactivate an embryonic neural crest state before initiating melanoma 3–5 Here we show the fate from within large fields adjacent abnormal melanocytes, which term “cancer precursor zone.” These cancer zone melanocytes have aberrant morphology, dysplastic nuclei, and altered gene expression. Using...
You have accessJournal of UrologyPlenary Session I - Best Abstracts1 Apr 2015PI-09 PRECLINICAL TESTING OF SMALL MOLECULE HIF2α INHIBITOR IN ZEBRAFISH AND MOUSE MODELS VHL-DEFICIENT RENAL CELL CARCINOMA. Meike Schneider, Ana Metelo, Haley Noonan, Xiang Li, Jing Youngnam, Nick Olson, Rania Baker, Lee Kamentsky, Yiyun Zhang, Anne Carpenter, Jing-Ruey Yeh, Randall Peterson, and Othon Iliopoulos SchneiderMeike Schneider More articles by this author , MeteloAna Metelo NoonanHaley Noonan LiXiang Li...
Developmental signaling pathways associated with growth factors such as TGFb are commonly dysregulated in melanoma. Here we identified a human enhancer specifically activated melanoma cells treated TGFB1 ligand. We generated stable transgenic zebrafish this Induced Enhancer driving green fluorescent protein ( TIE:EGFP ). was not expressed normal melanocytes or early melanomas but spatially distinct regions of advanced melanomas. Single cell RNA- sequencing revealed that + down-regulated...