A5039515443- Mitochondrial Function and Pathology
- ATP Synthase and ATPases Research
- Advanced Electron Microscopy Techniques and Applications
- Radiation Therapy and Dosimetry
- Metabolism and Genetic Disorders
- Lipid Membrane Structure and Behavior
- Photosynthetic Processes and Mechanisms
- melanin and skin pigmentation
- RNA modifications and cancer
- Sirtuins and Resveratrol in Medicine
- Nuclear Physics and Applications
- Redox biology and oxidative stress
Massachusetts General Hospital
2021-2024
Harvard University
2021
Abstract Cristae membrane state plays a central role in regulating mitochondrial function and cellular metabolism. The protein Optic atrophy 1 (Opa1) is an important crista remodeler that exists as two forms the mitochondrion, membrane-anchored long form (l-Opa1) processed short (s-Opa1). mechanisms for how Opa1 influences cristae shape have remained unclear due to lack of native three-dimensional views cristae. We perform situ cryo-electron tomography cryo-focused ion beam milled mouse...
Abstract Cristae membrane state plays a central role in regulating mitochondrial function and cellular metabolism. The protein Optic atrophy 1 (Opa1) is an important crista remodeler that exists as two forms the mitochondrion, membrane-anchored long form (l-Opa1) processed short (s-Opa1). mechanisms for how Opa1 influences cristae shape have remained unclear due to lack of native three-dimensional views cristae. We perform situ cryo-electron tomography cryo-focused ion beam milled mouse...
Cardiolipin is a tetra-acylated di-phosphatidylglycerol lipid enriched in the matrix-facing (inner) leaflet of mitochondrial inner membrane. plays an important role regulating mitochondria function and dynamics. Yet, mechanisms connecting cardiolipin distribution protein remain indirect. In our previous work, we established vitro system reconstituting membrane fusion mediated by Opa1. We found that long form Opa1 (l-Opa1) works together with proteolytically processed short (s-Opa1) to...
Mitochondrial fusion requires the sequential merger of four bilayers to two. The outer-membrane solute carrier family 25 member (SLC25A46) interacts with both outer and inner membrane dynamin GTPases mitofusin 1/2 optic atrophy 1 (Opa1). While SLC25A46 levels are known affect mitochondrial morphology, how Opa1 regulate is not understood. In this study, we use crosslinking mass spectrometry AlphaFold 2 modeling identify interfaces mediating an interaction Mfn2. We reveal that bundle signaling...
Mitochondrial fusion requires the sequential merger of four bilayers to two. The outer-membrane solute carrier protein SLC25A46 interacts with both outer and inner-membrane dynamin family GTPases Mfn1/2 Opa1. While levels are known affect mitochondrial morphology, how Opa1 regulate membrane is not understood. In this study, we use crosslinking mass-spectrometry AlphaFold 2 modeling identify interfaces mediating a interactions Mfn2. We reveal that bundle signaling element SLC25A46, present...
Abstract Cardiolipin is a tetra-acylated di-phosphatidylglycerol lipid enriched in the matrix-facing (inner) leaflet of mitochondrial inner membrane. plays an important role regulating mitochondria function and dynamics. Yet, mechanisms connecting cardiolipin distribution protein remain indirect. In our previous work, we established vitro system reconstituting membrane fusion mediated by Opa1. We found that long form Opa1 (l-Opa1) works together with proteolytically processed short (s-Opa1)...