A5101457766- Optical Imaging and Spectroscopy Techniques
- Spectroscopy Techniques in Biomedical and Chemical Research
- Photoacoustic and Ultrasonic Imaging
- Advanced Fluorescence Microscopy Techniques
- Optical Coherence Tomography Applications
- Spectroscopy and Chemometric Analyses
- Cellular Automata and Applications
- Nanoplatforms for cancer theranostics
- Advanced Data Storage Technologies
- Essential Oils and Antimicrobial Activity
- Thermoregulation and physiological responses
- Infrared Thermography in Medicine
- Semiconductor Lasers and Optical Devices
- melanin and skin pigmentation
- Photodynamic Therapy Research Studies
- Bee Products Chemical Analysis
- Cell Image Analysis Techniques
- Advanced Computational Techniques and Applications
- Plant nutrient uptake and metabolism
- Optical Systems and Laser Technology
- Optical Network Technologies
- Dermatologic Treatments and Research
- Non-Invasive Vital Sign Monitoring
- Mycorrhizal Fungi and Plant Interactions
- Legume Nitrogen Fixing Symbiosis
Harvard University
2016-2021
Massachusetts General Hospital
2016-2021
Anhui Agricultural University
2020
BC Cancer Agency
2010-2015
University of Washington
2014-2015
Tsinghua University
2008-2013
Vancouver Coastal Health Research Institute
2009-2012
University of British Columbia
2009-2012
Vancouver Coastal Health
2009-2012
Occupational Cancer Research Centre
2010-2012
Raman spectroscopy is a minimally-invasive optical technique with great potential for in vivo cancer detection and disease diagnosis. However, there no systematic study of the spectra from different organs to date. We measured characterized eighteen naïve mouse broad frequency range 700 3100 cm⁻¹. The peaks generic proteins lipids appeared all organs. Some like bone, teeth, brain lung had unique peaks. autofluorescence was strong liver, spleen, heart, kidney. These results suggest that organ...
Abstract Background and Objectives There has been a dramatic increase in photothermal therapy as minimally invasive treatment modality for cancer due to the development of novel nanomaterials light absorption agents. Single‐wall carbon nanotubes (SWNTs) with strong optical broad visible near IR offer unique advantages therapy. A range wavelengths can be used SWNTs, whereas conventional therapeutic agent is designed absorb only one selected wavelength. The objective this study validate...
We present a multimodal in vivo skin imaging instrument that is capable of simultaneously acquiring multiphoton and reflectance confocal images at up to 27 frames per second with 256 × pixel resolution without the use exogenous contrast agents. A single femtosecond laser excitation source used for all channels ensuring perfect image registration between two-photon fluorescence (TPF), harmonic generation (SHG), microscopy (RCM) images. Images videos acquired system show three provide...
Abstract Melanoma is the most deadly form of skin cancer with a yearly global incidence over 232,000 patients. Individuals fair and red hair exhibit highest risk for developing melanoma, evidence suggesting red/blond pigment known as pheomelanin may elevate melanoma through both UV radiation-dependent -independent mechanisms. Although ability to identify, characterize, monitor within vital improving our understanding underlying biology these lesions, no tools exist real-time, in vivo...
Abstract We have developed a micro‐Raman spectrometer system for use to differentiate tumor lesions from normal skin using an in vivo animal model. A study of 494 Raman spectra 24 mice revealed different spectral patterns at depths and between tumor‐bearing sites. peak 899 cm −1 (possibly proline or fatty acids) one with higher intensity the 1325–1330 range (assigned nucleic were correlated presence tumors, which can potentially be used as biomarkers cancer detection. Spectral diagnosis...
We present a multiphoton microscopy instrument specially designed for in vivo dermatological use that is capable of imaging human skin at 27 frames per second with 256 pixels×256 pixels resolution without the exogenous contrast agents. Imaging fast frame rates critical to reducing image blurring due patient motion and providing practically short clinical measurement times. Second harmonic generation two-photon fluorescence images videos acquired optimized wavelengths are presented showing...
The movement from the subjects during in vivo confocal Raman spectral measurements could change measurement volume, leading to non-specific signals and inaccurate interpretation of acquired spectrum. Here we introduce a generally applicable method that includes (1) developing multimodal system achieve real-time monitoring every with reflectance microscopy (RCM) multiphoton (MPM) imaging; (2) performing region-of-interest by scanning an area tissue acquisition. developed has been validated...
Vasculature response is a hallmark for most inflammatory skin disorders. Tape stripping on human causes minor inflammation which leads to changes in microvasculature. In this study, optical microangiography (OMAG), noninvasive volumetric microvasculature vivo imaging method, has been used track the vascular responses after tape stripping. Vessel density quantified and correlate with degree of irritation. The proved capability OMAG technique visualizing network under inflamed condition can...
Abstract Three-dimensional in vitro tumor models are highly useful tools for studying growth and treatment response of malignancies such as ovarian cancer. Existing viability assessment assays, however, face shortcomings when applied to these large, complex heterogeneous culture systems. Optical coherence tomography (OCT) is a noninvasive, label-free, optical imaging technique that can visualize live cells tissues over time with subcellular resolution millimeters penetration depth. Here, we...
Background/purposes: Understanding the two‐photon excitation spectral characteristics and microscopic morphology of cutaneous collagen elastic tissue components is important for applying multiphoton microscopy (MPM) in basic skin biology research clinical diagnosis. Methods: We developed a system measurements at various wavelengths. The was studied using commercial microscope. Results: obtained fluorescence (TPEF) excitation–emission matrices (EEM), first time, purified elastin samples, as...
Characterization of the relationship between external pressure and blood flow is important in examination pressure-induced disturbance tissue microcirculation. Optical coherence tomography (OCT)-based microangiography a promising imaging technique, capable providing noninvasive extraction functional vessels within skin with capillary-scale resolution. Here, we present feasibility study OCT (OMAG) to evaluate changes perfusion response externally applied on human vivo. External force loaded...
Lipids in stratum corneum are largely responsible for skin barrier function. There have been numerous studies on repairing and moisturizing effects of products containing occlusives, emollients humectants. However, currently there few systematically evaluating effect moisturizers endogenous lipids properties. The objective this study was to the various barrier-related indicators. A total 89 subjects with dry were enrolled. To evaluate test function hydration after 28 days use face, combined...
Background and Objective Faster better wound healing is a longstanding goal. Blood flow, angiogenesis, tissue oxygenation are important parameters in evaluating the process. Optical microangiography (OMAG) allows 3D imaging of vasculature can provide quantitative blood flow information down to capillary level resolution. Dual wavelength laser speckle (DW‐LSI) measure status. Materials Methods Cutaneous mouse ear model using multimodal system that combines OMAG with DWLSI was studied. Results...
Summary Background Skin cancer is the most common type of in humans. Current techniques for identifying normal and neoplastic tissues are either destructive or not sensitive specific enough. Raman spectroscopy confocal imaging may obviate many limitations existing methods by providing noninvasive, high‐resolution, real‐time morphological biochemical analysis living cells. Methods We conducted micro‐ R aman studies on HaCaT cells, melanocytes ( MC ) their malignant counterparts squamous cell...
Optical microangiography (OMAG) has been extensively utilized to study three-dimensional tissue vasculature in vivo. However, with the limited image resolution (∼10 μm) of commonly used systems, some concerns were raised: (1) whether OMAG is capable providing imaging capillary vessels that are an average diameter ∼6 μm; (2) if yes, can provide meaningful quantification vascular density within scanned volume. Multiphoton microscopy (MPM) depth-resolved high-resolution (∼1 biological...
Remote photoplethysmography (RPPG) can detect heart rate from facial videos in a non-contact way. However, head movement often affects its performance the real world. In this paper, novel anti-motion interference method named T-SNE-based signal separation (TSS) is proposed to solve problem. TSS first decomposes observed color traces into pulse-related vectors and noise using T-SNE algorithm. Then, it selects vector with most significant spectral peak as pulse for measurement. The tested on...
There are increased interests on using multiphoton imaging and spectroscopy for skin tissue characterization diagnosis. However, most studies have been done with just a few excitation wavelengths. Our objective is to perform systematic study of the two-photon fluorescence (TPF) properties fluorophores, normal skin, diseased tissues. A nonlinear excitation-emission-matrix (EEM) system guidance was constructed. tunable femtosecond laser used vary wavelengths from 730 920 nm EEM data...
Summary Background Recent advances in biomedical optics have enabled dermal and epidermal components to be visualized at subcellular resolution assessed noninvasively. Multiphoton microscopy ( MPM ) reflectance confocal RCM are noninvasive imaging modalities that demonstrated promising results skin micromorphology, which provide complementary information regarding components. This study assesses whether combined / can visualize intracellular extracellular melanin granules the epidermis...
Abstract not Available.
Abstract One‐photon absorption based traditional laser treatment may not necessarily be selective at the microscopic level, thus could result in un‐intended tissue damage. Our objective is to test whether two‐photon (TPA) provide highly targeted alteration of specific region interest without damaging surrounding tissues. TPA treatments (785 nm, 140 fs pulse width, 90 MHz) were performed on ex vivo mouse skin using different average power levels and irradiation times. Reflectance confocal...
Evaluation of spatiotemporal hemodynamic and metabolic responses during neural activation is crucial in studying brain function. We explore the use a noninvasive multifunctional optical imaging system to measure these mouse upon electrically stimulated activation, with cranium left intact. The developed by integrating an microangiography (OMAG) dual-wavelength laser speckle (DW-LSI) system. DW-LSI, running at image acquisition speed ∼100 Hz, used extract large-scale two-dimensional map,...
The movement from the subjects during in vivo confocal Raman spectral measurements could change measurement volume, leading to non-specific signals and inaccurate interpretation of acquired spectrum. Here we introduce a generally applicable method that includes (1) developing multimodal system achieve real-time monitoring every with reflectance microscopy (RCM) multiphoton (MPM) imaging; (2) performing region-of-interest by scanning an area tissue acquisition. developed has been validated...