A5006126677- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- CAR-T cell therapy research
- Reproductive System and Pregnancy
- IL-33, ST2, and ILC Pathways
- Hematopoietic Stem Cell Transplantation
- Cannabis and Cannabinoid Research
- Neuroscience and Neuropharmacology Research
- Multiple Sclerosis Research Studies
- Autoimmune and Inflammatory Disorders Research
- Immunotherapy and Immune Responses
- Medical Imaging Techniques and Applications
- Advanced Radiotherapy Techniques
- Ultrasound and Hyperthermia Applications
- Chronic Myeloid Leukemia Treatments
- Polyomavirus and related diseases
- Neurotransmitter Receptor Influence on Behavior
- Immune cells in cancer
- Neuroinflammation and Neurodegeneration Mechanisms
- Peripheral Neuropathies and Disorders
- Endometriosis Research and Treatment
- Tryptophan and brain disorders
- Cancer Immunotherapy and Biomarkers
- Psoriasis: Treatment and Pathogenesis
- Effects of Radiation Exposure
IRCCS Ospedale San Raffaele
2025
Ospedale Policlinico San Martino
2014-2023
University of Genoa
2012-2023
Heartspring
2020
Heinrich Heine University Düsseldorf
2016
Düsseldorf University Hospital
2016
Post Graduate Institute of Medical Education and Research
2016
Tecnologie Avanzate (Italy)
1995-2015
Zero to Three
2004-2015
Fondazione Santa Lucia
2011-2013
A small percentage of human T lymphocytes, predominantly CD8+ cells, express receptors for HLA class 1 molecules natural killer type (NK-R) that are inhibitory T-cell antigen receptor (TCR)-mediated functions. In the present study, it is demonstrated various NK-R typically expressed by NK cells also on periheral blood lymphocytes. These CD3+ NK-R+ have a cell surface phenotype typical memory as indicated expression CD45RO and CD29 lack CD28 CD45RA. Furthermore, combined use anti-TCR V...
A fraction of human T lymphocytes, predominantly CD8 + , express receptors for HLA class I molecules typical natural killer cells (natural or NKRs) that inhibit cell receptor-mediated functions. Herein, we analyzed possible mechanism(s) leading to the expression NKRs by responding superantigens allogeneic in vitro . We show that, presence interleukin 15 (IL-15), (depleted NKR cells) toxic shock syndrome toxin 1 de novo CD94, a molecule is part heterodimeric with broad specificity different...
Natural killer (NK) cells express surface receptors for defined groups of HLA class I alleles. The specific interaction between these and molecules expressed on target results in inhibition NK-mediated cell lysis. In this report, we analyzed whether similar mechanisms were operating cytolytic T lymphocytes (CTLs) capable lysing NK-sensitive cells. clones screened their ability to lyse K562 selected either gamma delta or alpha beta TCR. majority failed the I+ R8/15375 line; however, upon...
The ability of cannabinoids to modulate both inflammatory and degenerative neuronal damage prompted investigations on the potential benefits such compounds in multiple sclerosis (MS) animal models this disorder. Here we measured endocannabinoid levels, metabolism binding, physiological activities 26 patients with MS (17 females, aged 19-43 years), 25 healthy controls mice experimental autoimmune encephalomyelitis (EAE), a preclinical model MS. Our results show that EAE are associated...
Natural killer (NK) cells are the main lymphoid population in maternal decidua during first trimester of pregnancy. Decidual NK (dNK) display a unique functional profile and play key role promoting tissue remodeling, neoangiogenesis, immune modulation. However, little information exists on their origin development. Here we discovered CD34(+) hematopoietic precursors human (dCD34(+)). We show that dCD34(+) differ from cord blood- or peripheral blood-derived precursors. The expression...
Different HLA class I-specific killer inhibitory receptors (KIR) are expressed in vivo by a fraction of activated T cells, predominantly CD8+ , which they may inhibit TCR-mediated cell functions. In an attempt to identify mechanisms leading KIR expression we analyzed the effect transforming growth factor-β (TGF-β) cells responding bacterial superantigens vitro. We show that TGF-β induces CD94/NKG2A toxic shock syndrome toxin 1 or other staphylococcal superantigens. Remarkably, maximal CD94...
Abstract Immature postnatal thymocytes were shown to contain precursors which, under suitable culture conditions, give rise phenotypically and functionally mature natural killer (NK) cells. Here, we analyzed the effect of different cytokines for their ability induce expression HLA class I‐specific inhibitory receptor(s) during process NK cell development from immature thymocytes. From thymocyte suspensions depleted CD2 + , CD3 CD4 CD8 CD56 CD16 cells, further removed cells expressing...
P75/AIRM1 is a recently identified surface molecule that belongs to the sialoadhesin family and displays homology with myeloid cell antigen CD33. In lymphoid cells, p75/AIRM1 confined natural killer cells mediates inhibition of their cytolytic activity. this study, we show also expressed by myelomonocytic lineage, in which it appears at later stage as compared vitro proliferation differentiation cord blood-derived CD34 + (induced stem factor granulocyte–macrophage colony-stimulating factor)...
p75/AIRM-1 is a recently identified inhibitory receptor expressed by natural killer and myeloid cells displaying high homology with CD33. Crosslinking of or CD33 has been shown to sharply inhibit the in vitro proliferation both normal chronic leukemias. In this study, we analyzed acute leukemic for expression p75/AIRM-1. marked M5 (11/12) M4 (2/2) but not M1, M2, M3 subtypes according French–American–British classification. Cell samples from 12 leukemias were cultured presence...
BACKGROUND AND PURPOSE Alterations of glutamate-mediated synaptic transmission occur early during neuroinflammatory insults, and lead to degenerative neuronal damage in multiple sclerosis (MS) also experimental autoimmune encephalomyelitis (EAE), which is a murine model MS. Fingolimod an effective orally active agent for the treatment MS, affecting lymphocyte invasion brain. However, it still unclear if fingolimod can be neuroprotective this disorder. EXPERIMENTAL APPROACH Using...
Abstract Corticosteroids are known to inhibit NK cell functions. However no information is available on whether such inhibition may affect the expression and/or function of receptors involved in activation. In an attempt analyze this point, we studied peripheral blood cells isolated from pediatric patients undergoing allogeneic BM transplantation. were analyzed before, during and after methylprednisolone administration treat acute graft‐versus‐host disease. freshly treatment, surface...
The role of brain-derived neurotrophic factor (BDNF) in emotional processes suggests an interaction with the endocannabinoid system. Here, we addressed functional interplay between BDNF and cannabinoid CB<sub>1</sub> receptors (CB<sub>1</sub>Rs) striatum, a brain area which both CB<sub>1</sub>s play consequences stress rewarding experiences. potently inhibited CB<sub>1</sub>R function through mechanism mediated by altered cholesterol metabolism membrane lipid raft function. effect was...
Background: Inflammation triggers secondary neurodegeneration in multiple sclerosis (MS). Objectives: It is unclear whether classical anti-inflammatory cytokines have the potential to interfere with synaptic transmission and neuronal survival MS. Methods: Correlation analyses between cerebrospinal fluid (CSF) contents of molecular, imaging, clinical, neurophysiological measures alterations were performed. Results: Our data suggest that interleukin-13 (IL-13) plays a neuroprotective role MS...
Glutamate transmission is dysregulated in both multiple sclerosis (MS) and experimental autoimmune encephalomyelitis (EAE), the animal model of MS. A characteristic EAE increased glutamate associated with up-regulation AMPA receptors. However, little known about role NMDA receptors synaptic modifications induced by EAE.The contribution to alterations disease severity mice was assessed means neurophysiological, morphological, Western blot, metabolic clinical score assessments.In our mice,...
Background and purpose Multiple sclerosis ( MS ) patients discontinuing natalizumab are at risk of rebound disease activity. Methods In the present multi‐center, open‐label, non‐randomized, prospective, pilot study, we tested whether treatment with glatiramer acetate GA is safe effective after in patients. The study was performed academic tertiary medical centers. Forty active relapsing–remitting who never failed therapy discontinued 12–18 months were enrolled. initiated 4 weeks last dose...