Increased risk of cardiovascular disease in patients with chronic renal failure (CRF) has been explained by accelerated atherosclerosis and impaired angiogenesis, which endothelial progenitor cells (EPCs) may play key roles. We hypothesized that altered EPC biology contribute to the pathophysiology CRF.EPCs were isolated from CRF on maintenance hemodialysis (n=44) a normal control group (n=30). showed markedly decreased numbers (44.6%) colonies (75.3%) when compared controls (P<0.001). These...

Endothelial progenitor cells (EPCs) act as endothelial precursors that promote new blood vessel formation and increase angiogenesis by secreting growth factors cytokines in ischemic tissues. These facts prompt the hypothesis EPC transplantation should accelerate wound-repair process facilitating neovascularization production of various molecules related to wound healing. In a murine dermal excisional model, accelerated re-epithelialization compared with mature (ECs) control mice. When wounds...

Transplantation of human cord blood-derived endothelial progenitor cells (EPCs) is reported to contribute neovascularization in various ischemic diseases. However, the possible beneficial role and underlying mechanisms diabetes-impaired wound healing have been less well characterized. In this study, EPC transplantation stimulated keratinocyte fibroblast proliferation substantially as early 3 days after injury, leading significantly accelerated closure streptozotocin-induced diabetic nude...

Generation of induced pluripotent stem (iPS) cells has been intensively studied by a variety reprogramming methods, but the molecular and functional properties differentiated from iPS have not well characterized.To address this issue, we generated human aortic vascular smooth muscle (HASMCs) using lentiviral transduction defined transcription factors these back into (SMCs).Established were shown to possess equivalent embryonic cells, in terms cell surface markers, global mRNA microRNA...

Human induced pluripotent stem cells (hiPSCs) are generated through the reprogramming of somatic into an embryonic cell-like state, such that vascular differentiated from hiPSCs might be a suitable autologous cell source for regeneration. The goal this study was to assess whether cotransplantation endothelial (ECs) and smooth muscle (SMCs) could promote neovascularization tissue repair in murine dermal wound model. were ECs SMCs; displayed cell-specific surface markers. Compared primary...

Loss of BMP (bone morphogenic protein) signaling induces a phenotype switch pulmonary arterial smooth muscle cells (PASMCs), which is the pathological basis vascular remodeling in hypertension (PAH). Here, we identified FGF12 (fibroblast growth factor 12) as novel regulator BMP-induced change PASMCs and elucidated its role during PAH development. Using murine models lung specimens patients with PAH, observed that expression was significantly reduced PASMCs. In human PASMCs, increased by...

Previous studies on the role of stem cell factor (SCF) in endothelial progenitor (EPC)-mediated neovascularization have focused EPC mobilization and homing process. However, direct effects SCF activity EPCs not been characterized. We sought to determine whether regulates ability by comparing its roles mature cells.In vitro vivo assays revealed that substantially increased human through c-Kit receptor. Notably, SCF-induced increase was greater than umbilical vein cells (HUVECs)....

Aberrant neovascularization is a leading cause of blindness in several eye diseases, including age-related macular degeneration and proliferative diabetic retinopathy. The identification key regulators pathological ocular has been subject extensive research great therapeutic interest. Here, we explored the previously unrecognized role cKIT its ligand, SCF (stem cell factor), process. Approach Results: Compared with normoxia, hypoxia, crucial driver neovascularization, caused to be highly...

In hypoxia, endothelial cells (ECs) proliferate, migrate, and form new vasculature in a process called angiogenesis. Recent studies have suggested that ECs rely on glycolysis to meet metabolic needs for angiogenesis ischaemic tissues, several investigated the molecular mechanisms integrating metabolism. Here, we role of stem cell factor (SCF) its receptor, cKIT, regulating during hypoxia-driven

Objective—Increased risk of cardiovascular disease in patients with chronic renal failure (CRF) has been explained by accelerated atherosclerosis and impaired angiogenesis, which endothelial progenitor cells (EPCs) may play key roles. We hypothesized that altered EPC biology contribute to the pathophysiology CRF. Methods Results—EPCs were isolated from CRF on maintenance hemodialysis (n44) a normal control group (n30). showed markedly decreased numbers (44.6%) colonies (75.3%) when compared...

Purpose: Vascular endothelial growth factor (VEGF) signaling via VEGF receptor 2 (VEGFR2) plays a crucial role in pathologic ocular neovascularization. In this study, we investigated the antiangiogenic effect of apatinib, pharmacologic inhibitor VEGFR2 tyrosine kinase, against oxygen-induced retinopathy (OIR) and laser-induced choroidal neovascularization (CNV) mice. Methods: Western blotting vitro angiogenesis assays were performed using human retinal microvascular cells (HRMECs). OIR was...

Pathological angiogenesis is one of the major symptoms severe ocular diseases, including corneal neovascularization. The blockade vascular endothelial growth factor (VEGF) action has been recognized as an efficient strategy for treating In this study, we aimed to investigate whether nanoparticle-based delivery apatinib, a novel and selective inhibitor VEGF receptor 2, inhibits VEGF-mediated suppresses experimental Water-insoluble apatinib was encapsulated in nanoparticles composed human...

An angiogenesis assay based on gene transfer would be extremely useful for angiogenic therapy. A simple, reproducible, and quantitative to test genes provide more accurate predictions than conventional peptide-based assays. Here, we have developed a semiquantitative utilizing into skeletal muscle, which is target tissue ischemic limb diseases. To facilitate quick clean analysis, naked plasmid DNA vector combined with an electroporation procedure was used transfer. When the encoding vascular...

Pulmonary hypertension (PH) is characterized by structural and functional changes in the lung including proliferation of vascular smooth muscle cells (VSMCs) excessive collagen synthesis. Although connective tissue growth factor (CTGF) known to promote cell proliferation, migration, adhesion, extracellular matrix production various tissues, studies on role CTGF pulmonary have been limited. Here, we examined expression tissues male Sprague Dawley rats treated with monocrotaline (MCT, 60...

The endothelium has emerged recently as a therapeutic target in the treatment of hypertension because endothelial dysfunction and subsequent vascular rarefaction cause organ damage further elevate blood pressure (BP). It led us to hypothesize that one survival factors, potent derivative angiopoietin-1 (cartilage oligomeric matrix protein, COMP-Ang-1), could be novel class antihypertensive agents maintain integrity function, thereby preventing development damage. To study role COMP-Ang-1...

Our goal was to investigate the correlation between dysregulation of transforming growth factor-β1 (TGF-β1) and cystic medial degeneration in aortic aneurysmal tissues Marfan syndrome (MFS) patients. Although aneurysm animal models MFS is related TGF-β, it has yet be determined whether TGF-β correlates with pathogenic characteristics patients.Compared tissue from normal individuals, layers patients exhibited profound cellular apoptosis. These histopathologic changes positively correlated...

The effect of aldosterone on connective tissue growth factor (CTGF) was examined in rat embryonic ventricular myocytes. Upon treatment, CTGF expression significantly increased a dose and time-dependent manner. To explore the molecular mechanism for this upregulation, we role mineralocorticoid receptor. Pre-treatment an antagonist (spironolactone) at 5-fold excess blocked induction by aldosterone, suggesting that upregulation mediated Aldosterone treatment resulted activation ERK1/2, p38...

To evaluate the efficacy of curcumin-coating stent (CCS) on inhibition restenosis in a rabbit iliac artery model.Curcumin, pigment naturally acquired from rhizome plant curcuma longa, is known to have antiproliferative, antimigratory, and anti-inflammatory effects. However, it still unclear that curcumin can inhibit neointimal proliferation injured vessel.Dose-dependent cell growth was observed over dose range 10 nM microM. CCS prepared by dip-coating method (high-dose: HD, low-dose: LD)....

Diabetic retinopathy is the leading cause of blindness which associated with excessive angiogenesis. Using structure wondonin marine natural products, we previously created a scaffold to develop novel type antiangiogenesis agent that possesses minimized cytotoxicity. To overcome its poor pharmaceutical properties, further modified structure. A new was derived in stereogenic carbon changed nitrogen and 1,2,3-triazole ring replaced by an alkyl chain. By comparing bioactivity versus...

To test the hypothesis that overexpression of early growth response factor-1 (Egr-1) contributes to revascularization ischemic limbs, a constitutively active form Egr-1 (Egr-1*) was made and evaluated in vitro vivo. Analyses transduced myocytes revealed significant upregulation bFGF, PDGF-A, PDGF-B, IGF-II, TGF-β1. A coculture assay paracrine effects indicated Ad-Egr-1* promoted proliferation migration endothelial cells. When injected into tibialis anterior muscle mice, followed by explant...